Characterization of geometric variance in the epithelial nerve net of the ctenophore Pleurobrachia pileus.
Pleurobrachia pileus
ctenophore
image analysis
model organism
nerve net
neuroanatomy
Journal
The Journal of comparative neurology
ISSN: 1096-9861
Titre abrégé: J Comp Neurol
Pays: United States
ID NLM: 0406041
Informations de publication
Date de publication:
06 2022
06 2022
Historique:
revised:
23
11
2021
received:
14
04
2021
accepted:
15
12
2021
pubmed:
22
12
2021
medline:
16
4
2022
entrez:
21
12
2021
Statut:
ppublish
Résumé
Neuroscience lacks a diverse repertoire of model organisms, resulting in an incomplete understanding of the general principles of neural function. Ctenophores display many neurobiological and experimental features which make them a promising candidate to fill this gap. They possess a nerve net distributed across their body surface in the epithelial layer. There is a long-held assumption that nerve nets are "simple" and lack distinct organizational principles. We want to challenge this assumption and determine how stereotyped the structure of this network is. We estimated body surface area in Pleurobrachia pileus using custom optical projection tomography and light sheet morphometry imaging systems. Using an antibody against tyrosinated α-tubulin, we visualized the nerve net in situ and quantified the geometric properties using an automated segmentation approach. We characterized organizational rules of the epithelial nerve net in animals of different sizes and at different regions of the body. We found that specific morphological features within the nerve net are largely unchanged during growth. These properties must be essential to the functionality of the nervous system and therefore are maintained during a change in body size. We have also established the principles of organization of the network and showed that some of the geometric properties are variable across different parts of the body. This suggests that there may be different functions occurring in regions with different structural characteristics. This is the most comprehensive structural description of a ctenophore nerve net to date and demonstrates the amenability of P. pileus for whole organism network analysis.
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1438-1458Subventions
Organisme : School of Medicine, University College Dublin, Irish Research Council Government of Ireland Postgraduate Scholarship (GOIPG/2020/1459) and European Research Council (ERC) under the European Union's Horizon 2020 Research and Innovation Programme
ID : ERC-2014-CoG-646923-DBSModel
Informations de copyright
© 2021 Wiley Periodicals LLC.
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