Translesion DNA synthesis-driven mutagenesis in very early embryogenesis of fast cleaving embryos.
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
25 01 2022
25 01 2022
Historique:
accepted:
22
12
2021
revised:
22
10
2021
received:
17
06
2021
pubmed:
24
12
2021
medline:
22
2
2022
entrez:
23
12
2021
Statut:
ppublish
Résumé
In early embryogenesis of fast cleaving embryos, DNA synthesis is short and surveillance mechanisms preserving genome integrity are inefficient, implying the possible generation of mutations. We have analyzed mutagenesis in Xenopus laevis and Drosophila melanogaster early embryos. We report the occurrence of a high mutation rate in Xenopus and show that it is dependent upon the translesion DNA synthesis (TLS) master regulator Rad18. Unexpectedly, we observed a homology-directed repair contribution of Rad18 in reducing the mutation load. Genetic invalidation of TLS in the pre-blastoderm Drosophila embryo resulted in reduction of both the hatching rate and single-nucleotide variations on pericentromeric heterochromatin in adult flies. Altogether, these findings indicate that during very early Xenopus and Drosophila embryos TLS strongly contributes to the high mutation rate. This may constitute a previously unforeseen source of genetic diversity contributing to the polymorphisms of each individual with implications for genome evolution and species adaptation.
Identifiants
pubmed: 34939656
pii: 6481200
doi: 10.1093/nar/gkab1223
pmc: PMC8789082
doi:
Substances chimiques
Heterochromatin
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
885-898Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.
Références
J Cell Sci Suppl. 1989;12:149-60
pubmed: 2517560
EMBO J. 2004 Oct 1;23(19):3886-96
pubmed: 15359278
Fly (Austin). 2020 Mar - Dec;14(1-4):49-61
pubmed: 31933406
PLoS Genet. 2015 Mar 27;11(3):e1005092
pubmed: 25816335
Science. 1994 Mar 18;263(5153):1625-9
pubmed: 8128251
Biology (Basel). 2012 Dec 05;1(3):766-77
pubmed: 24832518
Nucleic Acids Res. 2019 Jan 8;47(D1):D1005-D1012
pubmed: 30445434
Dev Cell. 2004 Aug;7(2):275-81
pubmed: 15296723
J Biol Chem. 2016 Feb 19;291(8):3747-56
pubmed: 26740629
BMC Genomics. 2019 Aug 30;20(1):685
pubmed: 31470794
Nucleic Acids Res. 2019 Jan 8;47(D1):D941-D947
pubmed: 30371878
J Cell Biol. 2010 Apr 19;189(2):233-46
pubmed: 20385778
Nature. 1997 Jul 3;388(6637):93-7
pubmed: 9214509
Cell Cycle. 2013 Dec 15;12(24):3749-58
pubmed: 24107634
Genome Res. 2014 Jun;24(6):954-62
pubmed: 24614976
EMBO J. 2003 Dec 15;22(24):6621-30
pubmed: 14657033
Crit Rev Biochem Mol Biol. 2017 Jun;52(3):274-303
pubmed: 28279077
Nat Cell Biol. 2004 Jul;6(7):648-55
pubmed: 15220931
Nature. 2000 Apr 27;404(6781):1011-3
pubmed: 10801132
Mol Cell. 2008 May 23;30(4):519-29
pubmed: 18498753
J Exp Med. 2007 Aug 6;204(8):1989-98
pubmed: 17664295
DNA Repair (Amst). 2019 Oct;82:102688
pubmed: 31450086
Nat Rev Mol Cell Biol. 2017 Oct;18(10):622-636
pubmed: 28811666
Semin Cell Dev Biol. 2021 May;113:132-147
pubmed: 33500205
Cell Struct Funct. 2006;31(1):29-37
pubmed: 16565574
Mol Cell. 2015 Sep 17;59(6):998-1010
pubmed: 26365379
Mol Biol Cell. 1997 Jul;8(7):1195-206
pubmed: 9243501
Nat Cell Biol. 2009 May;11(5):592-603
pubmed: 19396164
Nat Commun. 2016 Mar 29;7:11165
pubmed: 27021558
Genetics. 1999 Apr;151(4):1531-45
pubmed: 10101175
Acta Biochim Pol. 2012;59(2):275-8
pubmed: 22693686
Genes Cells. 1999 Nov;4(11):607-18
pubmed: 10620008
Cell. 1984 Aug;38(1):55-64
pubmed: 6380762
Annu Rev Genet. 2014;48:269-94
pubmed: 25195504
BMC Evol Biol. 2014 Nov 25;14:233
pubmed: 25424548
J Biol Chem. 2001 May 4;276(18):15155-63
pubmed: 11297519
Nat Genet. 2016 Feb;48(2):126-133
pubmed: 26656846
Genes Dev. 2019 Jan 1;33(1-2):75-89
pubmed: 30567999
Nature. 2017 Mar 30;543(7647):714-718
pubmed: 28329761
Mol Cell Biol. 2001 Feb;21(3):940-51
pubmed: 11154280
Cell. 1980 Oct;21(3):761-71
pubmed: 6254667
Mol Cell Biol. 1993 Nov;13(11):6897-906
pubmed: 8413282
Sci Rep. 2018 Nov 1;8(1):16143
pubmed: 30385770
Nucleic Acids Res. 2017 Mar 17;45(5):2600-2614
pubmed: 27994034
Development. 2014 Mar;141(6):1332-41
pubmed: 24553286
Mol Biol Evol. 1998 Mar;15(3):293-302
pubmed: 9501496
Nucleic Acids Res. 2009 Jan;37(1):1-13
pubmed: 19033363
Genome Med. 2018 Apr 25;10(1):33
pubmed: 29695279
Curr Biol. 2014 Oct 6;24(19):2281-7
pubmed: 25242033
Mech Dev. 1997 Dec;69(1-2):183-95
pubmed: 9486540
G3 (Bethesda). 2019 May 7;9(5):1581-1595
pubmed: 30948422
Mol Cell. 2004 May 21;14(4):491-500
pubmed: 15149598
Nucleic Acids Res. 2009 Apr;37(7):2176-93
pubmed: 19228710
PLoS Genet. 2012 Jun;8(6):e1002800
pubmed: 22761594
Development. 2009 Feb;136(3):449-59
pubmed: 19141674
Trends Genet. 2017 Feb;33(2):86-100
pubmed: 28104289
Chromosoma. 2013 Mar;122(1-2):55-66
pubmed: 23149855
Nucleic Acids Res. 2012 Apr;40(8):3431-42
pubmed: 22187152
Cell. 2015 Mar 12;160(6):1169-81
pubmed: 25748651
Mol Carcinog. 2009 Apr;48(4):369-78
pubmed: 19117014
Nature. 2016 Oct 19;538(7625):336-343
pubmed: 27762356
Science. 2015 May 1;348(6234):1253671
pubmed: 25931565
Development. 2009 Mar;136(6):923-32
pubmed: 19234062
Genes (Basel). 2019 May 24;10(5):
pubmed: 31137726
Dev Cell. 2015 Aug 10;34(3):364-72
pubmed: 26212134
Oncogene. 2015 Aug 13;34(33):4403-11
pubmed: 25417706
J Cell Biol. 2013 Apr 29;201(3):395-408
pubmed: 23609533
J Cell Biol. 2006 Mar 27;172(7):999-1008
pubmed: 16549501
Curr Biol. 1999 Mar 25;9(6):302-12
pubmed: 10209095
Nucleic Acids Res. 2014;42(17):11071-82
pubmed: 25170086
Proc Natl Acad Sci U S A. 2000 Jul 5;97(14):7927-32
pubmed: 10884424