Chromatin-Independent Interplay of NFATc1 and EZH2 in Pancreatic Cancer.
Animals
Carcinoma, Pancreatic Ductal
/ genetics
Cell Proliferation
/ genetics
Chromatin
/ genetics
Disease Models, Animal
Enhancer of Zeste Homolog 2 Protein
/ genetics
Gene Expression Regulation, Neoplastic
/ genetics
Homeodomain Proteins
/ genetics
Humans
Mice
NFATC Transcription Factors
/ genetics
Pancreas
/ metabolism
Pancreatic Neoplasms
/ genetics
Protein Processing, Post-Translational
/ genetics
Proto-Oncogene Proteins p21(ras)
/ genetics
RNA, Small Interfering
/ genetics
Trans-Activators
/ genetics
EZH2
NFATc1
chromatin
pancreatic cancer
posttranslational EZH2 modification
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
08 12 2021
08 12 2021
Historique:
received:
31
10
2021
revised:
02
12
2021
accepted:
03
12
2021
entrez:
24
12
2021
pubmed:
25
12
2021
medline:
18
1
2022
Statut:
epublish
Résumé
The Nuclear Factor of Activated T-cells 1 (NFATc1) transcription factor and the methyltransferase Enhancer of Zeste Homolog 2 (EZH2) significantly contribute to the aggressive phenotype of pancreatic ductal adenocarcinoma (PDAC). Herein, we aimed at dissecting the mechanistic background of their interplay in PDAC progression. NFATc1 and EZH2 mRNA and protein expression and complex formation were determined in transgenic PDAC models and human PDAC specimens. NFATc1 binding on the NFATc1 targets the Our findings disclose a previously unknown NFATc1-EZH2 axis operational in the pancreas and provide mechanistic insights into the conditions fostering NFATc1:EZH2 complex formation in PDAC.
Sections du résumé
BACKGROUND
The Nuclear Factor of Activated T-cells 1 (NFATc1) transcription factor and the methyltransferase Enhancer of Zeste Homolog 2 (EZH2) significantly contribute to the aggressive phenotype of pancreatic ductal adenocarcinoma (PDAC). Herein, we aimed at dissecting the mechanistic background of their interplay in PDAC progression.
METHODS
NFATc1 and EZH2 mRNA and protein expression and complex formation were determined in transgenic PDAC models and human PDAC specimens. NFATc1 binding on the
RESULTS
NFATc1 targets the
CONCLUSION
Our findings disclose a previously unknown NFATc1-EZH2 axis operational in the pancreas and provide mechanistic insights into the conditions fostering NFATc1:EZH2 complex formation in PDAC.
Identifiants
pubmed: 34943970
pii: cells10123463
doi: 10.3390/cells10123463
pmc: PMC8700089
pii:
doi:
Substances chimiques
Chromatin
0
Homeodomain Proteins
0
NFATC Transcription Factors
0
Nfatc1 protein, mouse
0
RNA, Small Interfering
0
Trans-Activators
0
pancreatic and duodenal homeobox 1 protein
0
Enhancer of Zeste Homolog 2 Protein
EC 2.1.1.43
Ezh2 protein, mouse
EC 2.1.1.43
Hras protein, mouse
EC 3.6.5.2
Proto-Oncogene Proteins p21(ras)
EC 3.6.5.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : KFO5002
Organisme : German Cancer Aid
ID : 70112108
Organisme : German Cancer Aid
ID : 70112505
Organisme : German Cancer Aid
ID : 70113213
Organisme : German Cancer Aid
ID : 70112999
Organisme : Wilhelm-Sander-Stiftung
ID : 2017.107.1
Organisme : Volkswagen-Stiftung/Ministry for Culture and Science in Lower Saxony
ID : 11-76251-12-3/16
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