Chicken Immune Cell Assay to Model Adaptive Immune Responses In Vitro.
PBMCs
chicken
immunomodulating compounds
primary cell culture
Journal
Animals : an open access journal from MDPI
ISSN: 2076-2615
Titre abrégé: Animals (Basel)
Pays: Switzerland
ID NLM: 101635614
Informations de publication
Date de publication:
19 Dec 2021
19 Dec 2021
Historique:
received:
17
10
2021
revised:
14
12
2021
accepted:
15
12
2021
entrez:
24
12
2021
pubmed:
25
12
2021
medline:
25
12
2021
Statut:
epublish
Résumé
Knowledge about the modes of action of immunomodulating compounds such as pathogens, drugs, or feed additives, e.g., probiotics, gained through controlled but animal-related in vitro systems using primary cultured peripheral blood mononuclear cells (PBMCs) will allow the development of targeted nutrition strategies. Moreover, it could contribute to the prevention of infectious diseases and the usage of antimicrobials, and further promote the health of the animals. However, to our knowledge, a protocol for the isolation of PBMCs with reduced thrombocyte count from chicken blood and subsequent cell culture over several days to assess the effects of immunomodulating compounds is not available. Therefore, we established an optimized protocol for blood sampling and immune cell isolation, culture, and phenotyping for chicken PBMCs. For blood sampling commercial Na-citrate tubes revealed the highest count of vital cells compared to commercial Li-heparin (
Identifiants
pubmed: 34944374
pii: ani11123600
doi: 10.3390/ani11123600
pmc: PMC8697874
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Federal Ministry of Education and Research
ID : FKZ 031B0743
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