The Nuts and Bolts of SARS-CoV-2 Spike Receptor-Binding Domain Heterologous Expression.
COVID-19
SARS-CoV-2
heterologous expression
protein production
receptor-binding domain
spike protein
Journal
Biomolecules
ISSN: 2218-273X
Titre abrégé: Biomolecules
Pays: Switzerland
ID NLM: 101596414
Informations de publication
Date de publication:
02 12 2021
02 12 2021
Historique:
received:
28
10
2021
revised:
27
11
2021
accepted:
30
11
2021
entrez:
24
12
2021
pubmed:
25
12
2021
medline:
6
1
2022
Statut:
epublish
Résumé
COVID-19 is a highly infectious disease caused by a newly emerged coronavirus (SARS-CoV-2) that has rapidly progressed into a pandemic. This unprecedent emergency has stressed the significance of developing effective therapeutics to fight the current and future outbreaks. The receptor-binding domain (RBD) of the SARS-CoV-2 surface Spike protein is the main target for vaccines and represents a helpful "tool" to produce neutralizing antibodies or diagnostic kits. In this work, we provide a detailed characterization of the native RBD produced in three major model systems:
Identifiants
pubmed: 34944456
pii: biom11121812
doi: 10.3390/biom11121812
pmc: PMC8699011
pii:
doi:
Substances chimiques
Recombinant Proteins
0
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Regione Lazio - Lazio Innova
ID : POR FESR 2014-2020, Avviso Pubblico "EMERGENZA CORONAVIRUS E OLTRE", CUP F84E21000030006
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