Characterization of Genetic Heterogeneity in Recurrent Metastases of Renal Cell Carcinoma.

metastasis next-generation sequencing personalized therapy pharmacogenomics renal cell carcinoma

Journal

Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829

Informations de publication

Date de publication:
10 Dec 2021
Historique:
received: 03 11 2021
revised: 02 12 2021
accepted: 04 12 2021
entrez: 24 12 2021
pubmed: 25 12 2021
medline: 25 12 2021
Statut: epublish

Résumé

Metastatic renal cell carcinoma (RCC) exhibits poor prognosis. Better knowledge of distant metastases is crucial to foster personalized treatment strategies. Here, we aimed to investigate the genetic landscape of metastases, including synchronous and/or recurrent metastases to elucidate potential drug target genes and clinically relevant mutations in a real-world setting of patients. We assessed 81 metastases from 56 RCC patients, including synchronous and/or recurrent metastases of 19 patients. Samples were analysed through next-generation sequencing with a high coverage (~1000× mean coverage). We therefore established a novel sequencing panel comprising 32 genes with impact on RCC development. We observed a high frequency of mutations in known RCC driver genes (e.g., >40% carriers of

Identifiants

pubmed: 34944839
pii: cancers13246221
doi: 10.3390/cancers13246221
pmc: PMC8699544
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : Robert Bosch Stiftung (Stuttgart, Germany)
ID : NA
Organisme : Horizon 2020-PHC-2015
ID : U-PGx 668353
Organisme : Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence Strategy
ID : EXC 2180 - 390900677
Organisme : ICEPHA Graduate School Tuebingen-Stuttgart
ID : NA
Organisme : IZKF Program of the Medical Faculty Tuebingen
ID : NA

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Auteurs

Carolin Sauter-Meyerhoff (C)

Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, 70376 Stuttgart, Germany.

Regina Bohnert (R)

Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, 70376 Stuttgart, Germany.

Pascale Mazzola (P)

Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, 70376 Stuttgart, Germany.

Viktoria Stühler (V)

Department of Urology, University Hospital Tuebingen, 72076 Tuebingen, Germany.

Siarhei Kandabarau (S)

Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, 70376 Stuttgart, Germany.

Florian A Büttner (FA)

Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, 70376 Stuttgart, Germany.

Stefan Winter (S)

Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, 70376 Stuttgart, Germany.

Lisa Herrmann (L)

Department of Urology, University Hospital Tuebingen, 72076 Tuebingen, Germany.

Steffen Rausch (S)

Department of Urology, University Hospital Tuebingen, 72076 Tuebingen, Germany.

Jörg Hennenlotter (J)

Department of Urology, University Hospital Tuebingen, 72076 Tuebingen, Germany.

Falko Fend (F)

Institute of Pathology and Neuropathology, University Hospital Tuebingen, 72076 Tuebingen, Germany.

Marcus Scharpf (M)

Institute of Pathology and Neuropathology, University Hospital Tuebingen, 72076 Tuebingen, Germany.

Arnulf Stenzl (A)

Department of Urology, University Hospital Tuebingen, 72076 Tuebingen, Germany.

Stephan Ossowski (S)

Institute of Medical Genetics and Applied Genomics, University of Tuebingen, 72076 Tuebingen, Germany.

Jens Bedke (J)

Department of Urology, University Hospital Tuebingen, 72076 Tuebingen, Germany.
German Cancer Consortium (DKTK), Partner Site Tuebingen, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany.

Matthias Schwab (M)

Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, 70376 Stuttgart, Germany.
German Cancer Consortium (DKTK), Partner Site Tuebingen, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany.
Departments of Clinical Pharmacology, Pharmacy and Biochemistry, University of Tuebingen, 72076 Tuebingen, Germany.
Cluster of Excellence iFIT (EXC2180) "Image-Guided and Functionally Instructed Tumor Therapies", University of Tuebingen, 72076 Tuebingen, Germany.

Elke Schaeffeler (E)

Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, 70376 Stuttgart, Germany.
Cluster of Excellence iFIT (EXC2180) "Image-Guided and Functionally Instructed Tumor Therapies", University of Tuebingen, 72076 Tuebingen, Germany.

Classifications MeSH