Blood Bacterial DNA Load and Profiling Differ in Colorectal Cancer Patients Compared to Tumor-Free Controls.
bacterial 16S rRNA gene
bacterial translocation
case-control study
colon cancer diagnoses
microbiome
Journal
Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829
Informations de publication
Date de publication:
18 Dec 2021
18 Dec 2021
Historique:
received:
07
11
2021
revised:
14
12
2021
accepted:
15
12
2021
entrez:
24
12
2021
pubmed:
25
12
2021
medline:
25
12
2021
Statut:
epublish
Résumé
Inflammation and immunity are linked to intestinal adenoma (IA) and colorectal cancer (CRC) development. The gut microbiota is associated with CRC risk. Epithelial barrier dysfunction can occur, possibly leading to increased intestinal permeability in CRC patients. We conducted a case-control study including 100 incident histologically confirmed CRC cases, and 100 IA and 100 healthy subjects, matched to cases by center, sex and age. We performed 16S rRNA gene analysis of blood and applied conditional logistic regression. Further analyses were based on negative binomial distribution normalization and Random Forest algorithm. We found an overrepresentation of blood 16S rRNA gene copies in colon cancer as compared to tumor-free controls. For high levels of gene copies, community diversity was higher in colon cancer cases than controls. Bacterial taxa and operational taxonomic unit abundances were different between groups and were able to predict CRC with an accuracy of 0.70. Our data support the hypothesis of a higher passage of bacteria from gastrointestinal tract to bloodstream in colon cancer. This result can be applied on non-invasive diagnostic tests for colon cancer control.
Identifiants
pubmed: 34944982
pii: cancers13246363
doi: 10.3390/cancers13246363
pmc: PMC8699505
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Italian Association for Cancer Research
ID : 17070
Références
Front Cell Infect Microbiol. 2020 May 28;10:248
pubmed: 32547962
Int J Cancer. 2004 Jan 20;108(3):433-42
pubmed: 14648711
Front Oncol. 2021 Apr 15;11:626349
pubmed: 33937029
Microbiology (Reading). 2002 Jan;148(Pt 1):257-266
pubmed: 11782518
Lancet Infect Dis. 2013 Aug;13(8):719-24
pubmed: 23831427
Cancer Epidemiol Biomarkers Prev. 2003 Aug;12(8):755-62
pubmed: 12917207
Cancer Cell. 2021 May 10;39(5):708-724.e11
pubmed: 33798472
Gut. 2018 May;67(5):882-891
pubmed: 28341746
Sci Rep. 2016 Sep 28;6:33440
pubmed: 27678333
Bioinformatics. 2018 Apr 15;34(8):1287-1294
pubmed: 29228191
Br J Nutr. 2021 Jul 14;126(1):22-27
pubmed: 32962776
Microbiome. 2016 Jun 29;4(1):33
pubmed: 27357127
Nat Med. 2019 Apr;25(4):679-689
pubmed: 30936547
Cell Res. 2020 Jun;30(6):492-506
pubmed: 32433595
Transfusion. 2016 May;56(5):1138-47
pubmed: 26865079
J Biomed Sci. 2018 Nov 9;25(1):79
pubmed: 30413188
Gastroenterol Hepatol (N Y). 2017 Aug;13(8):476-483
pubmed: 28867979
Nat Med. 2019 Apr;25(4):667-678
pubmed: 30936548
Diabetes Care. 2014 Aug;37(8):2343-50
pubmed: 24824547
Sci Rep. 2017 Aug 17;7(1):8527
pubmed: 28819121
J Oncol. 2013;2013:703854
pubmed: 23577027
Biomed Res Int. 2020 Mar 19;2020:2948282
pubmed: 32280686
Clin J Am Soc Nephrol. 2019 May 7;14(5):692-701
pubmed: 30962186
Science. 2013 Mar 29;339(6127):1546-58
pubmed: 23539594
Ann Oncol. 2020 May;31(5):650-658
pubmed: 32321669
PLoS One. 2015 Nov 06;10(11):e0142334
pubmed: 26544955
Science. 2020 Oct 23;370(6515):467-472
pubmed: 33093110
CA Cancer J Clin. 2021 May;71(3):209-249
pubmed: 33538338
BMJ. 2015 Oct 06;351:h4970
pubmed: 26442928
Carcinogenesis. 2019 Jul 6;40(6):765-770
pubmed: 30753331
Front Cell Infect Microbiol. 2019 May 08;9:148
pubmed: 31139578
Hepatology. 2016 Dec;64(6):2015-2027
pubmed: 27639192
Gut. 2017 Jan;66(1):70-78
pubmed: 26408641
Gastroenterology. 2012 Aug;143(2):382-9
pubmed: 22609382
PLoS One. 2011;6(7):e22448
pubmed: 21829462
J Natl Cancer Inst. 2013 Dec 18;105(24):1907-11
pubmed: 24316595
Cancers (Basel). 2020 Apr 24;12(4):
pubmed: 32344707
Sci Rep. 2020 Jul 31;10(1):12932
pubmed: 32737354
J Natl Cancer Inst. 2021 Feb 1;113(2):109-111
pubmed: 32761203
PLoS One. 2019 Sep 11;14(9):e0206484
pubmed: 31509535
Cancer Treat Rev. 2015 Sep;41(8):671-9
pubmed: 26145760
Exp Mol Med. 2018 Aug 16;50(8):1-9
pubmed: 30115904
Gastroenterology. 2020 Jan;158(2):322-340
pubmed: 31586566
Nature. 2020 Mar;579(7800):567-574
pubmed: 32214244
Appl Environ Microbiol. 2013 Feb;79(4):1221-31
pubmed: 23220964
Gastroenterology. 2010 Jun;138(6):2101-2114.e5
pubmed: 20420949
Int J Mol Sci. 2018 Aug 30;19(9):
pubmed: 30200215
Eur J Surg Oncol. 2015 Mar;41(3):300-8
pubmed: 25468456
PLoS One. 2018 Nov 9;13(11):e0207002
pubmed: 30412600
Gut. 2019 Nov;68(11):1971-1978
pubmed: 31427405
Sci Rep. 2021 May 26;11(1):11054
pubmed: 34040086
Front Physiol. 2019 Jan 23;9:1942
pubmed: 30728783
mSystems. 2017 Dec 5;2(6):
pubmed: 29238752
Gut. 2018 Aug;67(8):1454-1463
pubmed: 28988196
Immunity. 2014 Jan 16;40(1):128-39
pubmed: 24412617
Cancer Lett. 2021 Feb 28;499:201-208
pubmed: 33249197
World J Gastroenterol. 2017 Mar 14;23(10):1909-1919
pubmed: 28348498