Tandem Use of OvMANE1 and Ov-16 ELISA Tests Increases the Sensitivity for the Diagnosis of Human Onchocerciasis.

ELISA Onchocerca volvulus Ov-16 OvMANE1 antibodies diagnostic sensitivity

Journal

Life (Basel, Switzerland)
ISSN: 2075-1729
Titre abrégé: Life (Basel)
Pays: Switzerland
ID NLM: 101580444

Informations de publication

Date de publication:
23 Nov 2021
Historique:
received: 18 10 2021
revised: 15 11 2021
accepted: 20 11 2021
entrez: 24 12 2021
pubmed: 25 12 2021
medline: 25 12 2021
Statut: epublish

Résumé

The current serological test for human onchocerciasis relies on IgG4 reactivity against the parasite Ov-16 antigen, with reported sensitivities of only 60-80%. As control programs move from control to elimination, it is imperative to identify novel molecules that could improve the serodiagnosis reliability of this disease. In this study we compared the sensitivity of total IgG against OvMANE1-a chimeric antigen previously identified as a potential biomarker of human onchocerciasis-with that of an Ov-16 antibody test to detect an

Identifiants

pubmed: 34947815
pii: life11121284
doi: 10.3390/life11121284
pmc: PMC8703281
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : European Research Council
ID : 671055
Pays : International
Organisme : VLIR-UOS
ID : Joint PhD Shintouo
Organisme : Académie de Recherche et d'Enseignement Supérieur
ID : ARES-PRD2013&2020Cameroun
Organisme : USAID and UK FCDO
ID : SGPIV/ 0121.070

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Auteurs

Cabirou Mounchili Shintouo (CM)

Department of Gerontology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium.
Frailty in Ageing Research Group, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium.
Department of Biochemistry and Molecular Biology, Faculty of Science, University of Buea, Buea P.O Box 63, Cameroon.

Stephen Mbigha Ghogomu (SM)

Department of Biochemistry and Molecular Biology, Faculty of Science, University of Buea, Buea P.O Box 63, Cameroon.

Robert Adamu Shey (RA)

Department of Biochemistry and Molecular Biology, Faculty of Science, University of Buea, Buea P.O Box 63, Cameroon.

An Hotterbeekx (A)

Global Health Institute, University of Antwerp, 2610 Antwerp, Belgium.

Emel Yagmur (E)

Department of Molecular Biology, Institute of Biology and Molecular Medicine, IBMM, Gosselies Campus, Université Libre de Bruxelles, 126040 Gosselies, Belgium.

Tony Mets (T)

Department of Geriatric Medicine, Universitair Ziekenhuis Brussel, Laarbeeklaan 101, 1090 Brussels, Belgium.

Luc Vanhamme (L)

Department of Molecular Biology, Institute of Biology and Molecular Medicine, IBMM, Gosselies Campus, Université Libre de Bruxelles, 126040 Gosselies, Belgium.

Robert Colebunders (R)

Global Health Institute, University of Antwerp, 2610 Antwerp, Belgium.

Jacob Souopgui (J)

Department of Molecular Biology, Institute of Biology and Molecular Medicine, IBMM, Gosselies Campus, Université Libre de Bruxelles, 126040 Gosselies, Belgium.

Rose Njemini (R)

Department of Gerontology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium.
Frailty in Ageing Research Group, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium.

Classifications MeSH