A modular platform for automated cryo-FIB workflows.

Animals Chlamydomonas reinhardtii Cryoelectron Microscopy / methods Drosophila melanogaster Electron Microscope Tomography / methods Haptophyta HeLa Cells Humans Saccharomyces cerevisiae Software Specimen Handling / methods
S. cerevisiae automation chlamydomonas reinhardtii correlative light and electron microscopy cryo-FIB-SEM cryo-electron tomography cryo-lift-out drosophila melanogaster electron microscopy emiliania huxleyi human in situ structural biology molecular biophysics structural biology

Journal

eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614

Informations de publication

Date de publication:
24 12 2021
Historique:
received: 18 05 2021
accepted: 23 12 2021
pubmed: 25 12 2021
medline: 21 1 2022
entrez: 24 12 2021
Statut: epublish

Résumé

Lamella micromachining by focused ion beam milling at cryogenic temperature (cryo-FIB) has matured into a preparation method widely used for cellular cryo-electron tomography. Due to the limited ablation rates of low Ga

Identifiants

DOI: 10.7554/eLife.70506 PMID: 34951584 PMC: PMC8769651
pubmed: 34951584
doi: 10.7554/eLife.70506
pii: 70506
pmc: PMC8769651
doi:
pii:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : European Research Council
ID : 760067
Pays : International

Informations de copyright

© 2021, Klumpe et al.

Déclaration de conflit d'intérêts

SK, HF, SG, IZ, BH, XZ, PE, JB, CM, MB, JM No competing interests declared, JP holds a position on the advisory board of Thermo Fisher Scientific

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Auteurs

Sven Klumpe (S)

Department Molecular Structural Biology, Max Planck Institute of Biochemistry, Martinsried, Germany.
ORCID: 0000-0002-8350-6503

Herman Kh Fung (HK)

Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
ORCID: 0000-0001-9568-1782

Sara K Goetz (SK)

Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
Collaboration for joint PhD degree between EMBL and Heidelberg University, Faculty of Biosciences, Heidelberg, Germany.
ORCID: 0000-0002-9903-3667

Ievgeniia Zagoriy (I)

Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.

Bernhard Hampoelz (B)

Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.

Xiaojie Zhang (X)

Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.

Philipp S Erdmann (PS)

Department Molecular Structural Biology, Max Planck Institute of Biochemistry, Martinsried, Germany.

Janina Baumbach (J)

Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.

Christoph W Müller (CW)

Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
ORCID: 0000-0003-2176-8337

Martin Beck (M)

Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
ORCID: 0000-0002-7397-1321

Jürgen M Plitzko (JM)

Department Molecular Structural Biology, Max Planck Institute of Biochemistry, Martinsried, Germany.
ORCID: 0000-0002-6402-8315

Julia Mahamid (J)

Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
ORCID: 0000-0001-6968-041X

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › A modular platform for automated cryo-FIB workflows.
questionsmedicales.fr › Eucaryotes › Viridiplantae › Chlorophyta › Chlamydomonas › Chlamydomonas reinhardtii › A modular platform for automated cryo-FIB workflows.
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Cryomicroscopie électronique › A modular platform for automated cryo-FIB workflows.
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Insectes › Pterygota › Neoptera › Holometabola › Diptera › Drosophilidae › Drosophila › Drosophila melanogaster › A modular platform for automated cryo-FIB workflows.
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Microscopie électronique à transmission › Tomographie en microscopie électronique › A modular platform for automated cryo-FIB workflows.
questionsmedicales.fr › Eucaryotes › Haptophyta › A modular platform for automated cryo-FIB workflows.
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HeLa › A modular platform for automated cryo-FIB workflows.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › A modular platform for automated cryo-FIB workflows.
questionsmedicales.fr › Eucaryotes › Champignons › Levures › Saccharomyces › Saccharomyces cerevisiae › A modular platform for automated cryo-FIB workflows.
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Logiciel › A modular platform for automated cryo-FIB workflows.
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Manipulation d'échantillons › A modular platform for automated cryo-FIB workflows.