Comparative Diagnostic Performance of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Kit for the Rapid Detection of SARS-CoV-2.
COVID-19
RT-LAMP
RT-PCR
SARS-CoV-2
diagnostic accuracy
Journal
Pathogens (Basel, Switzerland)
ISSN: 2076-0817
Titre abrégé: Pathogens
Pays: Switzerland
ID NLM: 101596317
Informations de publication
Date de publication:
15 Dec 2021
15 Dec 2021
Historique:
received:
26
11
2021
revised:
09
12
2021
accepted:
14
12
2021
entrez:
28
12
2021
pubmed:
29
12
2021
medline:
29
12
2021
Statut:
epublish
Résumé
Although the reverse transcription-polymerase chain reaction (RT-PCR) is considered a standard-of-care assay for the laboratory diagnosis of SARS-CoV-2, several limitations of this method have been described. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is an alternative molecular assay and is potentially able to overcome some intrinsic shortcomings of RT-PCR. In this study, we evaluated the diagnostic performance of the novel HG COVID-19 RT-LAMP assay. In this retrospective analysis, a total of 400 routinely collected leftover nasopharyngeal samples with a known RT-PCR result were tested by means of the HG COVID-19 RT-LAMP assay. The overall sensitivity and specificity values of HG COVID-19 RT-LAMP versus RT-PCR were 97.0% (95% CI: 93.6-98.9%) and 98.5% (95% CI: 95.7-99.7%), respectively. Inter-assay agreement was almost perfect (
Identifiants
pubmed: 34959584
pii: pathogens10121629
doi: 10.3390/pathogens10121629
pmc: PMC8706056
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : European Commission
ID : 101003713
Références
Nucleic Acids Res. 2000 Jun 15;28(12):E63
pubmed: 10871386
Int J Infect Dis. 2021 Jun;107:215-218
pubmed: 33930540
Sci Rep. 2020 Dec 18;10(1):22349
pubmed: 33339871
J Infect. 2021 Mar;82(3):e35-e37
pubmed: 33248218
Euro Surveill. 2021 Mar;26(10):
pubmed: 33706862
Arch Virol. 2021 Oct;166(10):2825-2828
pubmed: 34302551
Clin Microbiol Infect. 2020 Sep;26(9):1178-1182
pubmed: 32593741
PLoS One. 2020 Dec 10;15(12):e0242958
pubmed: 33301459
JAMA. 2021 May 25;325(20):2115-2116
pubmed: 33830171
Biosens Bioelectron. 2020 Oct 1;165:112454
pubmed: 32729549
Int J Infect Dis. 2021 Mar;104:303-305
pubmed: 33434671
Infect Dis Ther. 2020 Sep;9(3):573-586
pubmed: 32725536
Cochrane Database Syst Rev. 2021 Mar 24;3:CD013705
pubmed: 33760236
Biology (Basel). 2020 Jul 22;9(8):
pubmed: 32707972
BMJ. 2020 May 12;369:m1808
pubmed: 32398230
BMJ Open Respir Res. 2021 Feb;8(1):
pubmed: 33627333
Am J Infect Control. 2021 Jan;49(1):21-29
pubmed: 32659413
Infect Drug Resist. 2020 Aug 03;13:2657-2665
pubmed: 32801804
BMJ Open. 2016 Nov 14;6(11):e012799
pubmed: 28137831
Expert Rev Mol Diagn. 2021 Jan;21(1):43-61
pubmed: 33474990
Microbiol Spectr. 2021 Dec 22;9(3):e0149421
pubmed: 34787486
J Infect Chemother. 2009 Apr;15(2):62-9
pubmed: 19396514
J Clin Pathol. 2020 Jul;73(7):370-377
pubmed: 32404473
Med Hypotheses. 2020 Aug;141:109786
pubmed: 32361529
Euro Surveill. 2020 Aug;25(32):
pubmed: 32794447