Continuous organelle separation in an insulator-based dielectrophoretic device.
continuous separation
insulator-based dielectrophoresis
mitochondria
ratchet
size-based separation
Journal
Electrophoresis
ISSN: 1522-2683
Titre abrégé: Electrophoresis
Pays: Germany
ID NLM: 8204476
Informations de publication
Date de publication:
06 2022
06 2022
Historique:
revised:
30
11
2021
received:
04
10
2021
accepted:
13
12
2021
pubmed:
30
12
2021
medline:
18
6
2022
entrez:
29
12
2021
Statut:
ppublish
Résumé
Heterogeneity in organelle size has been associated with devastating human maladies such as neurodegenerative diseases or cancer. Therefore, assessing the size-based subpopulation of organelles is imperative to understand the biomolecular foundations of these diseases. Here, we demonstrated a ratchet migration mechanism using insulator-based dielectrophoresis in conjunction with a continuous flow component that allows the size-based separation of submicrometer particles. The ratchet mechanism was realized in a microfluidic device exhibiting an array of insulating posts, tailoring electrokinetic and dielectrophoretic transport. A numerical model was developed to elucidate the particle migration and the size-based separation in various conditions. Experimentally, the size-based separation of a mixture of polystyrene beads (0.28 and 0.87
Identifiants
pubmed: 34964147
doi: 10.1002/elps.202100326
doi:
Substances chimiques
Polystyrenes
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1283-1296Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM127562
Pays : United States
Informations de copyright
© 2022 Wiley-VCH GmbH.
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