Cross Talk between α7 and α3β4 Nicotinic Receptors Prevents Their Desensitization in Human Chromaffin Cells.
chromaffin cell
human
nicotinic receptor
patch-clamp
α3β4
α7
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
ISSN: 1529-2401
Titre abrégé: J Neurosci
Pays: United States
ID NLM: 8102140
Informations de publication
Date de publication:
16 02 2022
16 02 2022
Historique:
received:
24
05
2021
revised:
24
11
2021
accepted:
05
12
2021
pubmed:
31
12
2021
medline:
26
2
2022
entrez:
30
12
2021
Statut:
ppublish
Résumé
The physical interaction and functional cross talk among the different subtypes of neuronal nicotinic acetylcholine receptors (nAChRs) expressed in the various tissues is unknown. Here, we have investigated this issue between the only two nAChRs subtypes expressed, the α7 and α3β4 subtypes, in a human native neuroendocrine cell (the chromaffin cell) using electrophysiological patch-clamp, fluorescence, and Förster resonance energy transfer (FRET) techniques. Our data show that α7 and α3β4 receptor subtypes require their mutual and maximal efficacy of activation to increase their expression, to avoid their desensitization, and therefore, to increase their activity. In this way, after repetitive stimulation with acetylcholine (ACh), α7 and α3β4 receptor subtypes do not desensitize, but they do with choline. The nicotinic current increase associated with the α3β4 subtype is dependent on Ca
Identifiants
pubmed: 34965978
pii: JNEUROSCI.1115-21.2021
doi: 10.1523/JNEUROSCI.1115-21.2021
pmc: PMC8883849
doi:
Substances chimiques
Receptors, Nicotinic
0
alpha7 Nicotinic Acetylcholine Receptor
0
nicotinic receptor alpha3beta4
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1173-1183Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM103801
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM136430
Pays : United States
Informations de copyright
Copyright © 2022 the authors.
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