A fast, aqueous, reversible three-day tissue clearing method for adult and embryonic mouse brain and whole body.
Journal
Cell reports methods
ISSN: 2667-2375
Titre abrégé: Cell Rep Methods
Pays: United States
ID NLM: 9918227360606676
Informations de publication
Date de publication:
22 11 2021
22 11 2021
Historique:
entrez:
30
12
2021
pubmed:
31
12
2021
medline:
31
12
2021
Statut:
ppublish
Résumé
Optical clearing methods serve as powerful tools to study intact organs and neuronal circuits. We developed an aqueous clearing protocol, Fast 3D Clear, that relies on tetrahydrofuran for tissue delipidation and iohexol for clearing, such that tissues can be imaged under immersion oil in light-sheet imaging systems. Fast 3D Clear requires 3 days to achieve high transparency of adult and embryonic mouse tissues while maintaining their anatomical integrity and preserving a vast array of transgenic and viral/dye fluorophores. A unique advantage of Fast 3D Clear is its complete reversibility and thus compatibility with tissue sectioning and immunohistochemistry. Fast 3D Clear can be easily and quickly applied to a wide range of biomedical studies, facilitating the acquisition of high-resolution two- and three-dimensional images.
Identifiants
pubmed: 34966901
doi: 10.1016/j.crmeth.2021.100090
pmc: PMC8713566
mid: NIHMS1761974
doi:
Substances chimiques
Fluorescent Dyes
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
100090Subventions
Organisme : Howard Hughes Medical Institute
Pays : United States
Déclaration de conflit d'intérêts
DECLARATION OF INTERESTS The authors declare no competing interests. A patent has been filed by S.K., A.N., A.L., and E.R.K., and the Zuckerman Institute.
Références
Nat Protoc. 2012 Nov;7(11):1983-95
pubmed: 23060243
Cell Res. 2019 Oct;29(10):870-872
pubmed: 31444467
Eur J Histochem. 2014 Dec 12;58(4):2461
pubmed: 25578980
Development. 1991 Aug;112(4):1053-61
pubmed: 1682128
J Biol Chem. 2012 Sep 28;287(40):33512-22
pubmed: 22893709
Am J Clin Pathol. 1959 Apr;31(4):357-61
pubmed: 13637049
Cell. 2014 Nov 6;159(4):911-24
pubmed: 25417165
J Comp Neurol. 2018 Feb 15;526(3):373-396
pubmed: 29063602
Cell Res. 2018 Aug;28(8):803-818
pubmed: 29844583
Cell Rep. 2018 Oct 23;25(4):959-973.e6
pubmed: 30355501
J Neurosci. 2012 Feb 29;32(9):3131-41
pubmed: 22378886
Cell. 2016 Jun 16;165(7):1789-1802
pubmed: 27238021
Neuron. 2017 Jan 4;93(1):33-47
pubmed: 27989459
Int J Biochem Cell Biol. 2017 Mar;84:35-39
pubmed: 28082099
Sci China Life Sci. 2014 Mar;57(3):269-274
pubmed: 24390420
Sci Adv. 2019 Jan 11;5(1):eaau8355
pubmed: 30746463
J Anat. 2021 Feb;238(2):489-507
pubmed: 32939792
PLoS One. 2015 May 20;10(5):e0124650
pubmed: 25993380
Nat Methods. 2016 Oct;13(10):859-67
pubmed: 27548807
Ann Anat. 2001 Jan;183(1):91-5
pubmed: 11206989
Development. 2014 Apr;141(8):1726-36
pubmed: 24715462
Cell. 1996 Dec 27;87(7):1317-26
pubmed: 8980237
J Biophotonics. 2019 Aug;12(8):e201800368
pubmed: 30932329
Cell. 2014 Nov 6;159(4):896-910
pubmed: 25417164
Nat Methods. 2012 Jul;9(7):671-5
pubmed: 22930834
Int J Neuropsychopharmacol. 2014 Oct 31;18(1):
pubmed: 25522378
Cell. 2014 Aug 14;158(4):945-958
pubmed: 25088144
Nat Methods. 2013 Jun;10(6):508-13
pubmed: 23722210
Front Syst Neurosci. 2017 Jun 28;11:46
pubmed: 28701931
Neuron. 2013 Jun 5;78(5):773-84
pubmed: 23764283
Sci Rep. 2018 Jan 31;8(1):1964
pubmed: 29386656