Cell-surface glycans act as attachment factors for porcine hemagglutinating encephalomyelitis virus.

Animals Betacoronavirus 1 Cell Line Cell Membrane Polysaccharides Swine Virus Attachment
Attachment factor Coronavirus Heparan sulfate Porcine hemagglutinating encephalomyelitis virus Sialic acid

Journal

Veterinary microbiology
ISSN: 1873-2542
Titre abrégé: Vet Microbiol
Pays: Netherlands
ID NLM: 7705469

Informations de publication

Date de publication:
Feb 2022
Historique:
received: 06 10 2021
revised: 10 12 2021
accepted: 19 12 2021
pubmed: 1 1 2022
medline: 28 1 2022
entrez: 31 12 2021
Statut: ppublish

Résumé

Porcine hemagglutinating encephalomyelitis virus (PHEV) is a neurotropic coronavirus and highly pathogenic in veterinary clinic. Spike (S) protein of PHEV interplays with host components to cross the plasma membrane of target cells, but characterization of its functional receptors is limited. Here, we discovered that cell-surface glycans, i.e., sialic acid (SA) and heparan sulfate (HS), act as critical interacting factors of PHEV, involving in viral attachment. As shown in glycans depletion assay, removing SA or HS from N2a cells inhibits PHEV infection. Soluble sugar monomers were utilized for competitive binding tests, and we found that both SA and HS could specifically bind to PHEV and affect the viral infectivity. Furthermore, the expression of heparan sulfate proteoglycans (HSPGs), including syndecans and glypicans, and endoglycosidase heparinase which cleaves HS were regulated by PHEV RNA replication. Together, we newly identified specificity recognition of cellular glycans and PHEV during infection, providing novel cellular targets for antiviral therapies and better understanding of pathogenesis.

Identifiants

DOI: 10.1016/j.vetmic.2021.109315 PMID: 34972029
pubmed: 34972029
pii: S0378-1135(21)00338-2
doi: 10.1016/j.vetmic.2021.109315
pii:
doi:

Substances chimiques

Polysaccharides 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

109315

Informations de copyright

Copyright © 2021 Elsevier B.V. All rights reserved.

Auteurs

Shiyu Hu (S)

Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China.

Kui Zhao (K)

Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China.

Yungang Lan (Y)

Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China.

Junchao Shi (J)

Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China.

Jiyu Guan (J)

Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China.

Huijun Lu (H)

Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, China.

Feng Gao (F)

Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China.

Haihua Feng (H)

Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China.

Wenqi He (W)

Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China.

Zi Li (Z)

Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China; Key Lab of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, China. Electronic address: lizi1227@jlu.edu.cn.

Articles similaires

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Sudhir Prem Srivastava, Vishwajyoti Pascual Srivastava, Avinesh Singh et al.
1.00
Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Use of organic material provided by an automatic enrichment device by weaner pigs and its influence on tail lesions.

Philipp Heseker, Jeanette Probst, Stefanie Ammer et al.
1.00
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Cell-surface glycans act as attachment factors...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN à brin positif › Nidovirales › Coronaviridae › Coronavirus › Betacoronavirus › Betacoronavirus-1 › Cell-surface glycans act as attachment factors...
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › Cell-surface glycans act as attachment factors...
questionsmedicales.fr › Cellules › Structures cellulaires › Membrane cellulaire › Cell-surface glycans act as attachment factors...
questionsmedicales.fr › Glucides › Polyosides › Cell-surface glycans act as attachment factors...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Suidae › Cell-surface glycans act as attachment factors...
questionsmedicales.fr › Phénomènes microbiologiques › Phénomènes physiologiques viraux › Attachement viral › Cell-surface glycans act as attachment factors...