Preparation of Platelet-Rich Plasma: National IADVL PRP Taskforce Recommendations.
Centrifuge
PRP kits
RCF
RPM
guidelines
platelet-rich plasma
preparation
recommendations
regenerative medicine
standardization
Journal
Indian dermatology online journal
ISSN: 2229-5178
Titre abrégé: Indian Dermatol Online J
Pays: India
ID NLM: 101586880
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
received:
29
04
2021
revised:
06
06
2021
accepted:
08
06
2021
entrez:
3
1
2022
pubmed:
4
1
2022
medline:
4
1
2022
Statut:
epublish
Résumé
The goal of these recommendations is to provide a framework to practitioners for implementing useful, evidence-based recommendations for the preparation of platelet-rich plasma (PRP) in various dermatological indications. The Indian Association of Dermatologists, Venereologists and Leprologists (IADVL) assigned the task of preparing these recommendations to its task force on PRP. A comprehensive literature search was done in the English language on the preparation of PRP across multiple databases. The grade of evidence and strength of recommendation was evaluated on the GRADE framework (Grading of Recommendation, Assessment, Development and Evaluation). A draft of clinical recommendations was developed on the best available evidence which was also scrutinized and critically evaluated by the IADVL Academy of Dermatology. Based on the inputs received, this final consensus statement was prepared. A total of 45 articles (meta-analyses, prospective and retrospective studies, reviews [including chapters in books], and case series) were critically evaluated and the evidence thus gathered was used in the preparation of these recommendations. This expert group recommends the use of double-spin manual method for the preparation of PRP. The recommended parameters for centrifuge are 100-300 g for 5-10 min for the first spin and 400-700 g for 10-17 min for the second spin. The recommended platelet concentration in PRP for the treatment of various dermatological conditions is 1-1.5 million platelets/μL. The activation of PRP is not required when it is injected into soft tissues.
Identifiants
pubmed: 34976877
doi: 10.4103/idoj.idoj_269_21
pii: IDOJ-12-12
pmc: PMC8664176
doi:
Types de publication
Journal Article
Review
Langues
eng
Pagination
S12-S23Informations de copyright
Copyright: © 2021 Indian Dermatology Online Journal.
Déclaration de conflit d'intérêts
There are no conflicts of interest.
Références
GMS Interdiscip Plast Reconstr Surg DGPW. 2014 Nov 24;3:Doc11
pubmed: 26504722
Platelets. 2004 Aug;15(5):315-8
pubmed: 15370102
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 Mar;101(3):e56-60
pubmed: 16504852
Implant Dent. 2001;10(4):225-8
pubmed: 11813662
Sci Rep. 2017 Apr 25;7(1):1125
pubmed: 28442778
Clin Oral Investig. 2019 May;23(5):2179-2185
pubmed: 30280327
Anesthesiology. 1998 Jun;88(6):1579-85
pubmed: 9637652
J Clin Invest. 1969 Jun;48(6):1073-82
pubmed: 5771188
Indian Dermatol Online J. 2017 Nov-Dec;8(6):460-464
pubmed: 29204389
J Cutan Aesthet Surg. 2014 Oct-Dec;7(4):213-9
pubmed: 25722600
BMJ Open Sport Exerc Med. 2016 Feb 4;2(1):e000060
pubmed: 27900152
Skin Appendage Disord. 2018 Jan;4(1):1-11
pubmed: 29457005
Biores Open Access. 2012 Jun;1(3):115-23
pubmed: 23516671
Indian Dermatol Online J. 2020 Jul 13;11(4):575-579
pubmed: 32832445
J Oral Maxillofac Surg. 2007 Jun;65(6):1084-93
pubmed: 17517290
Stem Cell Res Ther. 2013 Jun 07;4(3):67
pubmed: 23759113
Blood Coagul Fibrinolysis. 2006 Oct;17(7):557-61
pubmed: 16988551
Biomed Res Int. 2016;2016:6591717
pubmed: 27672658
Skin Appendage Disord. 2018 Jan;4(1):18-24
pubmed: 29457008
Transfusion. 2009 Apr;49(4):771-8
pubmed: 19170984
Platelets. 2020 Jul 3;31(5):661-666
pubmed: 31498027
Wounds. 2014 Aug;26(8):232-8
pubmed: 25860639
Clin Chem. 2004 Nov;50(11):2215-6
pubmed: 15502103
Lab Invest. 2001 Apr;81(4):581-92
pubmed: 11304578
Eur Spine J. 2004 Oct;13 Suppl 1:S102-6
pubmed: 15221571
J Cosmet Dermatol. 2018 Oct;17(5):666-671
pubmed: 29790267
Int J Mol Sci. 2018 Jan 10;19(1):
pubmed: 29320421
Plast Reconstr Surg Glob Open. 2014 Jul 09;2(6):e162
pubmed: 25289355
Clinics (Sao Paulo). 2019;74:e1132
pubmed: 31433042
Tissue Eng Part C Methods. 2012 Mar;18(3):176-85
pubmed: 21951067
J Oral Implantol. 2013 Oct;39(5):525-32
pubmed: 21480780
Clin Chem. 2010 Sep;56(9):1483-91
pubmed: 20663962
Am J Sports Med. 2011 Oct;39(10):2135-40
pubmed: 21846925
Blood. 1968 Aug;32(2):324-35
pubmed: 5672853
BMJ. 2004 Jun 19;328(7454):1490
pubmed: 15205295
Br J Haematol. 1999 Jan;104(1):64-8
pubmed: 10027713
BMC Microbiol. 2013 Feb 25;13:47
pubmed: 23442413
HSS J. 2017 Feb;13(1):75-80
pubmed: 28167878
J Oral Maxillofac Surg. 2000 Mar;58(3):297-300; discussion 300-1
pubmed: 10716112
PLoS One. 2017 Dec 8;12(12):e0188921
pubmed: 29220362
Tissue Eng Part A. 2019 Nov;25(21-22):1550-1563
pubmed: 30896295
ISRN Hematol. 2014 Mar 25;2014:176060
pubmed: 25006472
BMJ. 2008 May 10;336(7652):1049-51
pubmed: 18467413
J Vis Exp. 2013 Apr 09;(74):
pubmed: 23609458
Stem Cells Int. 2016;2016:7414036
pubmed: 27340410
Clin Biochem. 2009 Sep;42(13-14):1452-60
pubmed: 19560449
Vox Sang. 2009 Aug;97(2):110-8
pubmed: 19392780
J Cutan Aesthet Surg. 2014 Oct-Dec;7(4):189-97
pubmed: 25722595
Clin Oral Investig. 2017 Nov;21(8):2619-2627
pubmed: 28154995
Bone. 2004 Apr;34(4):665-71
pubmed: 15050897
Int J Mol Sci. 2017 Feb 14;18(2):
pubmed: 28216604