Hybridoma Screening by Antigen Capture: Reverse Dot Blot.

Animals Antibodies, Monoclonal Clone Cells Collodion Enzyme-Linked Immunosorbent Assay Horseradish Peroxidase Hybridomas Immunoblotting Mice Rabbits

Journal

Cold Spring Harbor protocols
ISSN: 1559-6095
Titre abrégé: Cold Spring Harb Protoc
Pays: United States
ID NLM: 101524530

Informations de publication

Date de publication:
04 01 2022
Historique:
entrez: 5 1 2022
pubmed: 6 1 2022
medline: 23 4 2022
Statut: epublish

Résumé

A dot blot is widely used to determine the productivity of a given hybridoma. This assay can also be used to screen a fusion or subclone plate for productive hybridoma clones. First, a nitrocellulose membrane is coated with an affinity-purified goat or rabbit anti-mouse immunoglobulin and then incubated with hybridoma tissue culture supernatant. Monoclonal antibodies in the supernatant are then "captured" on the coated nitrocellulose membrane surface and detected by screening with horseradish peroxidase (HRP).

Identifiants

DOI: 10.1101/pdb.prot103135 PMID: 34983859
pubmed: 34983859
pii: 2022/1/pdb.prot103135
doi: 10.1101/pdb.prot103135
doi:

Substances chimiques

Antibodies, Monoclonal 0
Collodion 9004-70-0
Horseradish Peroxidase EC 1.11.1.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2022 Cold Spring Harbor Laboratory Press.

Auteurs

Edward A Greenfield (EA)

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Hybridoma Screening by Antigen Capture:...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps monoclonaux › Hybridoma Screening by Antigen Capture:...
questionsmedicales.fr › Cellules › Cellules cultivées › Clones cellulaires › Hybridoma Screening by Antigen Capture:...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Biopolymères › Cellulose › Collodion › Hybridoma Screening by Antigen Capture:...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Techniques d'immunoadsorption › Test ELISA › Hybridoma Screening by Antigen Capture:...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Peroxidases › Horseradish peroxidase › Hybridoma Screening by Antigen Capture:...
questionsmedicales.fr › Cellules › Cellules cultivées › Cellules hybrides › Hybridomes › Hybridoma Screening by Antigen Capture:...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Immunotransfert › Hybridoma Screening by Antigen Capture:...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Hybridoma Screening by Antigen Capture:...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Lagomorpha › Lapins › Hybridoma Screening by Antigen Capture:...