Characterization of the O-Glycoproteome of Porphyromonas gingivalis.
O-glycosylation
Porphyromonas gingivalis
biosynthesis
glycans
glycoproteins
mass spectrometry
proteome
trifluoromethanesulfonic acid
Journal
Microbiology spectrum
ISSN: 2165-0497
Titre abrégé: Microbiol Spectr
Pays: United States
ID NLM: 101634614
Informations de publication
Date de publication:
23 02 2022
23 02 2022
Historique:
pubmed:
6
1
2022
medline:
17
3
2022
entrez:
5
1
2022
Statut:
ppublish
Résumé
Porphyromonas gingivalis is an important human pathogen and also a model organism for the Bacteroidetes phylum. O-glycosylation has been reported in this phylum with findings that include the O-glycosylation motif, the structure of the O-glycans in a few species, and an extensive O-glycoproteome analysis in Tannerella forsythia. However, O-glycosylation has not yet been confirmed in P. gingivalis. We therefore used glycoproteomics approaches including partial deglycosylation with trifluoromethanesulfonic acid as well as both HILIC and FAIMS based glycopeptide enrichment strategies leading to the identification of 257 putative glycosylation sites in 145 glycoproteins. The sequence of the major O-glycan was elucidated to be HexNAc-HexNAc(P-Gro-[Ac]
Identifiants
pubmed: 34985300
doi: 10.1128/spectrum.01502-21
pmc: PMC8729774
doi:
Substances chimiques
Bacterial Proteins
0
Glycoproteins
0
Polysaccharides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0150221Références
Periodontol 2000. 2010 Oct;54(1):15-44
pubmed: 20712631
J Proteome Res. 2021 Jan 1;20(1):599-612
pubmed: 33125241
J Med Microbiol. 2007 Nov;56(Pt 11):1536-1548
pubmed: 17965357
J Clin Periodontol. 2000 Sep;27(9):648-57
pubmed: 10983598
BMC Microbiol. 2010 May 25;10:152
pubmed: 20500879
Sci Adv. 2019 Jan 23;5(1):eaau3333
pubmed: 30746447
Periodontol 2000. 2020 Jun;83(1):175-188
pubmed: 32385886
Eur J Biochem. 2001 Sep;268(17):4748-57
pubmed: 11532011
J Biol Chem. 2011 Nov 4;286(44):38714-38724
pubmed: 21911490
Microb Pathog. 2016 May;94:35-41
pubmed: 26456570
mSphere. 2021 Oct 27;6(5):e0064921
pubmed: 34523981
Mol Microbiol. 2011 Mar;79(5):1380-401
pubmed: 21244528
Glycobiology. 2013 May;23(5):568-77
pubmed: 23258847
Cell. 2016 Apr 21;165(3):690-703
pubmed: 27062925
Microbiol Mol Biol Rev. 2020 Jan 2;84(1):
pubmed: 31896547
Periodontol 2000. 2020 Jun;83(1):107-124
pubmed: 32385887
Biochem Biophys Res Commun. 2008 Jan 25;365(4):784-9
pubmed: 18029265
J Proteome Res. 2018 Jul 6;17(7):2377-2389
pubmed: 29766714
Glycobiology. 2019 Aug 20;29(9):620-624
pubmed: 31184695
J Clin Periodontol. 1998 Feb;25(2):134-44
pubmed: 9495612
Infect Immun. 2014 Nov;82(11):4563-71
pubmed: 25135681
Infect Immun. 1999 Aug;67(8):3816-23
pubmed: 10417143
J Bacteriol. 2010 Aug;192(16):4103-10
pubmed: 20562309
Front Microbiol. 2018 Aug 28;9:2008
pubmed: 30210478
J Biol Chem. 1995 Jun 2;270(22):13197-203
pubmed: 7768917
J Periodontol. 2013 Apr;84(4 Suppl):S8-S19
pubmed: 23631586
Microbiologyopen. 2013 Jun;2(3):383-401
pubmed: 23509024
J Proteome Res. 2014 May 2;13(5):2420-32
pubmed: 24620993
Sci Rep. 2020 Apr 30;10(1):7384
pubmed: 32355178
Mol Cell Proteomics. 2007 Nov;6(11):1942-51
pubmed: 17673454
PLoS Pathog. 2015 Sep 04;11(9):e1005152
pubmed: 26340749
J Periodontol. 2000 Oct;71(10):1554-60
pubmed: 11063387
Microbiologyopen. 2017 Feb;6(1):
pubmed: 27595778
Mol Oral Microbiol. 2018 Feb;33(1):68-80
pubmed: 28972686
PLoS One. 2011;6(6):e21372
pubmed: 21731719
Mol Oral Microbiol. 2021 Feb;36(1):25-36
pubmed: 33124778
Nat Microbiol. 2020 Aug;5(8):1016-1025
pubmed: 32393857
Appl Environ Microbiol. 2015 Jan;81(2):783-93
pubmed: 25398868
Mol Oral Microbiol. 2012 Dec;27(6):458-70
pubmed: 23134611
Science. 1988 Jan 1;239(4835):55-7
pubmed: 3336774
Mol Microbiol. 2013 May;88(4):772-83
pubmed: 23551589
J Biol Chem. 2012 Jul 13;287(29):24605-17
pubmed: 22593568
Methods Mol Biol. 2015;1295:355-68
pubmed: 25820733
Sci Rep. 2014 May 23;4:5056
pubmed: 24852504
Biochem J. 2002 Apr 1;363(Pt 1):105-15
pubmed: 11903053
Microbiol Immunol. 2019 Jan;63(1):11-20
pubmed: 30599082
Annu Rev Microbiol. 2017 Sep 8;71:539-556
pubmed: 28886680
J Biol Chem. 2011 Feb 4;286(5):3219-26
pubmed: 21115495
Cell Host Microbe. 2011 Nov 17;10(5):497-506
pubmed: 22036469
DNA Res. 2008 Aug;15(4):215-25
pubmed: 18524787
J Proteome Res. 2013 Oct 4;12(10):4449-61
pubmed: 24007199
Cell. 2009 Apr 17;137(2):321-31
pubmed: 19379697
Mol Cell Proteomics. 2021;20:100011
pubmed: 33578083
mBio. 2020 Sep 1;11(5):
pubmed: 32873758
Interface Focus. 2019 Apr 6;9(2):20180064
pubmed: 30842870