DMBT1 expression and neutrophil-to-lymphocyte ratio during necrotizing enterocolitis are influenced by impaired perfusion due to cardiac anomalies.
Congenital heart disease
DMBT1
Deleted in malignant brain tumors 1
Monocyte-to-lymphocyte ratio
Necrotizing enterocolitis
Neutrophil-to-lymphocyte ratio
Journal
Molecular and cellular pediatrics
ISSN: 2194-7791
Titre abrégé: Mol Cell Pediatr
Pays: Germany
ID NLM: 101660689
Informations de publication
Date de publication:
06 Jan 2022
06 Jan 2022
Historique:
received:
08
06
2021
accepted:
15
12
2021
entrez:
6
1
2022
pubmed:
7
1
2022
medline:
7
1
2022
Statut:
epublish
Résumé
Deleted in malignant brain tumors 1 (DMBT1) is involved in innate immunity and epithelial differentiation. It has been proven to play a role in various states of inflammation or hypoxia of fetal gastrointestinal and pulmonary diseases. Discrimination of pathogenesis in necrotizing enterocolitis (NEC) based on cardiac status improves the understanding of NEC in different patient subgroups. We aimed at examining DMBT1 expressions regarding their association with cardiac status leading to impaired intestinal perfusion, intraoperative bacteria proof, and a fulminant course of NEC. Twenty-eight patients with NEC were treated surgically between 2010 and 2019 at our institution. DMBT1 expression was examined in intestinal sections using immunohistochemistry to detect DMBT1 protein. Associations of clinical parameters and DMBT1 expression were analyzed. We examined DMBT1 levels in 10 patients without cardiac defects and 18 patients with persisting ductus arteriosus (PDA) and congenital heart defects (CHD). Compared to patients without cardiac malformations, DMBT1 levels tended to score higher in patients with PDA/CHD (p = 0.2113) and were negatively correlated with C-reactive protein in these infants (p = 0.0172; r = - 0.5533). The number of DMBT1-expressing macrophages was elevated in the PDA/CHD-subgroup (p = 0.0399). Ratios of neutrophils and monocytes to lymphocytes were significantly higher in infants with PDA/CHD (p = 0.0319 and 0.0493). DMBT1 expression was significantly associated with positive bacterial culture of intraoperative swabs (p = 0.0252) and DMBT1 expression of the serosa was associated with a fulminant course of NEC (p = 0.0239). This study demonstrates that DMBT1 expression may be influenced by cardiac anomalies with an impaired intestinal perfusion in the neonatal intestine. NEC in PDA/CHD infants is associated with more DMBT1-positive macrophages and a significantly elevated neutrophil-to-lymphocyte ratio.
Sections du résumé
BACKGROUND
BACKGROUND
Deleted in malignant brain tumors 1 (DMBT1) is involved in innate immunity and epithelial differentiation. It has been proven to play a role in various states of inflammation or hypoxia of fetal gastrointestinal and pulmonary diseases. Discrimination of pathogenesis in necrotizing enterocolitis (NEC) based on cardiac status improves the understanding of NEC in different patient subgroups. We aimed at examining DMBT1 expressions regarding their association with cardiac status leading to impaired intestinal perfusion, intraoperative bacteria proof, and a fulminant course of NEC.
METHODS
METHODS
Twenty-eight patients with NEC were treated surgically between 2010 and 2019 at our institution. DMBT1 expression was examined in intestinal sections using immunohistochemistry to detect DMBT1 protein. Associations of clinical parameters and DMBT1 expression were analyzed.
RESULTS
RESULTS
We examined DMBT1 levels in 10 patients without cardiac defects and 18 patients with persisting ductus arteriosus (PDA) and congenital heart defects (CHD). Compared to patients without cardiac malformations, DMBT1 levels tended to score higher in patients with PDA/CHD (p = 0.2113) and were negatively correlated with C-reactive protein in these infants (p = 0.0172; r = - 0.5533). The number of DMBT1-expressing macrophages was elevated in the PDA/CHD-subgroup (p = 0.0399). Ratios of neutrophils and monocytes to lymphocytes were significantly higher in infants with PDA/CHD (p = 0.0319 and 0.0493). DMBT1 expression was significantly associated with positive bacterial culture of intraoperative swabs (p = 0.0252) and DMBT1 expression of the serosa was associated with a fulminant course of NEC (p = 0.0239).
CONCLUSIONS
CONCLUSIONS
This study demonstrates that DMBT1 expression may be influenced by cardiac anomalies with an impaired intestinal perfusion in the neonatal intestine. NEC in PDA/CHD infants is associated with more DMBT1-positive macrophages and a significantly elevated neutrophil-to-lymphocyte ratio.
Identifiants
pubmed: 34989914
doi: 10.1186/s40348-021-00133-9
pii: 10.1186/s40348-021-00133-9
pmc: PMC8739415
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1Informations de copyright
© 2022. The Author(s).
Références
Front Pediatr. 2017 Mar 03;5:40
pubmed: 28316967
Am J Physiol Gastrointest Liver Physiol. 2012 Jul;303(1):G93-102
pubmed: 22538401
Clin Exp Immunol. 2008 Jan;151(1):123-9
pubmed: 17991292
J Matern Fetal Neonatal Med. 2019 Sep;32(18):3026-3033
pubmed: 29557695
Int J Clin Lab Res. 1992;22(1):1-4
pubmed: 1633313
Front Pediatr. 2020 Jun 05;8:257
pubmed: 32582588
Clin Perinatol. 2015 Sep;42(3):567-85
pubmed: 26250918
J Pediatr Gastroenterol Nutr. 2006 Sep;43(3):284-90
pubmed: 16954948
Eur J Immunol. 2003 Aug;33(8):2327-36
pubmed: 12884308
Histopathology. 1996 Jul;29(1):89-92
pubmed: 8818703
Ann Surg. 1978 Jan;187(1):1-7
pubmed: 413500
Mediators Inflamm. 2018 Jul 15;2018:3758068
pubmed: 30116146
BMC Res Notes. 2017 Jan 3;10(1):12
pubmed: 28057051
Pediatr Pulmonol. 2020 Nov;55(11):2964-2969
pubmed: 32770804
Innate Immun. 2010 Jun;16(3):160-7
pubmed: 20418254
Front Pediatr. 2018 Oct 23;6:312
pubmed: 30406064
Cancer Res. 2000 Mar 15;60(6):1704-10
pubmed: 10749143
Semin Perinatol. 2017 Feb;41(1):15-28
pubmed: 27940091
Histochem Cell Biol. 2016 Feb;145(2):227-37
pubmed: 26542257
Histochem Cell Biol. 2017 Mar;147(3):389-397
pubmed: 27628193
Gastroenterology. 2007 Nov;133(5):1499-509
pubmed: 17983803
J Pediatr Surg. 2017 Oct 12;:
pubmed: 29111080
Neonatology. 2020;117(2):240-244
pubmed: 32155645
BMC Pulm Med. 2015 Apr 08;15:32
pubmed: 25885541
Respir Res. 2007 Oct 01;8:69
pubmed: 17908325
J Pediatr Surg. 2009 Jun;44(6):1072-5; discussion 1075-6
pubmed: 19524719
J Pediatr Surg. 2019 Sep;54(9):1755-1760
pubmed: 30635129
Life Sci. 2008 Feb 13;82(7-8):341-7
pubmed: 18187159
Expert Rev Mol Med. 2016 Jun 24;18:e12
pubmed: 27341512
J Biol Chem. 2004 Nov 12;279(46):47699-703
pubmed: 15355985
Pediatr Res. 1992 Aug;32(2):145-9
pubmed: 1508603
J Leukoc Biol. 2006 Sep;80(3):492-9
pubmed: 16793909
Eur J Pediatr Surg. 2019 Feb;29(1):14-22
pubmed: 30112744
Am J Physiol Gastrointest Liver Physiol. 2009 Jul;297(1):G1-G10
pubmed: 19443732
Front Pediatr. 2021 Jan 06;8:593926
pubmed: 33490000