Immobilization of fibrinolytic protease from Mucor subtilissimus UCP 1262 in magnetic nanoparticles.

Chromatography, Ion Exchange Enzyme Stability Enzymes, Immobilized / chemistry Fibrinogen / chemistry Fibrinolytic Agents / chemistry Fungal Proteins / chemistry Humans Hydrogen-Ion Concentration Magnetite Nanoparticles / chemistry Mucor / chemistry Peptide Hydrolases / chemistry Temperature
Fibrinolytic enzyme Magnetic nanoparticles Toxicity

Journal

Protein expression and purification
ISSN: 1096-0279
Titre abrégé: Protein Expr Purif
Pays: United States
ID NLM: 9101496

Informations de publication

Date de publication:
04 2022
Historique:
received: 08 02 2021
revised: 15 11 2021
accepted: 03 01 2022
pubmed: 10 1 2022
medline: 19 2 2022
entrez: 9 1 2022
Statut: ppublish

Résumé

This work reports the immobilization of a fibrinolytic protease (FP) from Mucor subtilissimus UCP 1262 on Fe

Identifiants

DOI: 10.1016/j.pep.2022.106044 PMID: 34998976
pubmed: 34998976
pii: S1046-5928(22)00001-8
doi: 10.1016/j.pep.2022.106044
pii:
doi:

Substances chimiques

Enzymes, Immobilized 0
Fibrinolytic Agents 0
Fungal Proteins 0
Magnetite Nanoparticles 0
Fibrinogen 9001-32-5
Peptide Hydrolases EC 3.4.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

106044

Informations de copyright

Copyright © 2022 Elsevier Inc. All rights reserved.

Auteurs

Marllyn Marques da Silva (M)

Laboratory of Nanotechnology, Biotechnology and Cell Culture, Academic Center of Vitória, Federal University of Pernambuco, 55608-680, Vitória de Santo Antão, Pernambuco, Brazil.

José Manoel Wanderley Duarte Neto (JM)

Laboratory of Bioactive Technology, Department of Morphology and Animal Physiology, Federal Rural University of Pernambuco, Rua Dom Manoel de Medeiros, s/n, Dois Irmãos, 52171-900, Recife, Pernambuco, Brazil.

Bruno Vinícius Barros Regueira (BV)

Laboratory of Research in Biotechnology and Hemoderivatives, Department of Pharmaceutical Sciences, Federal University of Pernambuco, 50670-420, Recife, Pernambuco, Brazil.

Milena Tereza Torres do Couto (MT)

Laboratory of Research in Biotechnology and Hemoderivatives, Department of Pharmaceutical Sciences, Federal University of Pernambuco, 50670-420, Recife, Pernambuco, Brazil.

Renata Vitória da Silva Sobral (R)

Laboratory of Research in Biotechnology and Hemoderivatives, Department of Pharmaceutical Sciences, Federal University of Pernambuco, 50670-420, Recife, Pernambuco, Brazil.

Amanda Emmanuelle Sales Conniff (AE)

Department of Molecular Medicine- College of Medicine, University of South Florida, Bruce B. Downs Blvd, MDC 3518, 12901, Tampa, FL, United States.

Romero Marcos Pedrosa Brandão Costa (RM)

Laboratory of Advances in Protein Biotechnology (LABIOPROT), Institute of Biological Sciences, University of Pernambuco, Rua Arnóbio Marquês, 310 - Santo Amaro, Recife - PE, 50100-130, Recife, Pernambuco, Brazil.

Mariane Cajubá de Britto Lira Nogueira (M)

Laboratory of Nanotechnology, Biotechnology and Cell Culture, Academic Center of Vitória, Federal University of Pernambuco, 55608-680, Vitória de Santo Antão, Pernambuco, Brazil.

Noemia Pereira da Silva Santos (N)

Laboratory of Nanotechnology, Biotechnology and Cell Culture, Academic Center of Vitória, Federal University of Pernambuco, 55608-680, Vitória de Santo Antão, Pernambuco, Brazil.

Lorenzo Pastrana (L)

International Iberian Nanotechnology Laboratory, Av. Mestre José Veiga, Braga, 4715-330, Portugal.

Ana Cristina Lima Leite (AC)

Laboratory of Research in Biotechnology and Hemoderivatives, Department of Pharmaceutical Sciences, Federal University of Pernambuco, 50670-420, Recife, Pernambuco, Brazil.

Attilio Converti (A)

Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, University of Genoa, Via Opera Pia 15, I-16145 Genoa, Italy.

Thiago Pajeú Nascimento (TP)

Laboratory of Bioactive Technology, Department of Morphology and Animal Physiology, Federal Rural University of Pernambuco, Rua Dom Manoel de Medeiros, s/n, Dois Irmãos, 52171-900, Recife, Pernambuco, Brazil.

Ana Lúcia Figueiredo Porto (AL)

Laboratory of Bioactive Technology, Department of Morphology and Animal Physiology, Federal Rural University of Pernambuco, Rua Dom Manoel de Medeiros, s/n, Dois Irmãos, 52171-900, Recife, Pernambuco, Brazil. Electronic address: analuporto@yahoo.com.br.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie d'échange d'ions › Immobilization of fibrinolytic protease from...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Stabilité protéique › Stabilité enzymatique › Immobilization of fibrinolytic protease from...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines immobilisées › Enzymes immobilisées › Immobilization of fibrinolytic protease from...
questionsmedicales.fr › Facteurs biologiques › Facteurs de la coagulation sanguine › Fibrinogène › Immobilization of fibrinolytic protease from...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Agents hématologiques › Fibrinolytiques › Immobilization of fibrinolytic protease from...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines fongiques › Immobilization of fibrinolytic protease from...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Immobilization of fibrinolytic protease from...
questionsmedicales.fr › Phénomènes chimiques › Concentration en ions d'hydrogène › Immobilization of fibrinolytic protease from...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Nanostructures › Nanoparticules › Nanoparticules métalliques › Nanoparticules magnétiques d'oxyde de fer › Nanoparticules de magnétite › Immobilization of fibrinolytic protease from...
questionsmedicales.fr › Eucaryotes › Champignons › Champignons non classés › Mucorales › Mucor › Immobilization of fibrinolytic protease from...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Peptide hydrolases › Immobilization of fibrinolytic protease from...
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › Immobilization of fibrinolytic protease from...