SmartAmp method can rapidly detect SARS-CoV-2 in dead bodies.
Autopsy
Rapid test
SARS-CoV-2
SmartAmp method
Swab test
Journal
Forensic science international
ISSN: 1872-6283
Titre abrégé: Forensic Sci Int
Pays: Ireland
ID NLM: 7902034
Informations de publication
Date de publication:
Feb 2022
Feb 2022
Historique:
received:
19
10
2021
accepted:
21
12
2021
pubmed:
10
1
2022
medline:
28
1
2022
entrez:
9
1
2022
Statut:
ppublish
Résumé
Rapid and accurate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in dead bodies is essential to prevent infection among those working with dead bodies. This study focused on the Smart Amplification (SmartAmp) method, which has a short examination time (approximately an hour), is simple to perform, and demonstrates high specificity and sensitivity. This method has already been used for clinical specimens; however, its effectiveness in dead bodies has not been reported. This study examined the SmartAmp method using 11 autopsies or postmortem needle biopsies performed from January to May, 2021 (of these, five cases tested positive for SARS-CoV-2 by quantitative real-time polymerase chain reaction (qRT-PCR) and six cases tested negative). Swab samples were collected from the nasopharynx, oropharynx, or anus and the SmartAmp and qRT-PCR results were compared. For the nasopharynx and oropharynx samples, the same results were obtained for both methods in all cases; however, for the anal swabs, there was one case that was positive according to qRT-PCR but negative according to the SmartAmp method. The SmartAmp method may therefore be less sensitive than qRT-PCR and results may differ in specimens with a low viral load, such as anal swabs. However, in the nasopharynx and oropharynx specimens, which are normally used for testing, the results were the same using each method, suggesting that the SmartAmp method is useful in dead bodies. In the future, the SmartAmp method may be applied not only during autopsies, but also in various situations where dead bodies are handled.
Identifiants
pubmed: 34999365
pii: S0379-0738(21)00488-6
doi: 10.1016/j.forsciint.2021.111168
pmc: PMC8701773
pii:
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
111168Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Conflict of interests The authors declare no conflicts of interest associated with this manuscript.
Références
Nat Methods. 2007 Mar;4(3):257-62
pubmed: 17322893
Trends Biotechnol. 2011 May;29(5):240-50
pubmed: 21377748
Nucleosides Nucleotides Nucleic Acids. 2008 Mar;27(3):224-43
pubmed: 18260008
Angew Chem Int Ed Engl. 2009;48(35):6480-4
pubmed: 19637175
JMA J. 2021 Apr 15;4(2):67-75
pubmed: 33997438
PLoS One. 2012;7(1):e30236
pubmed: 22295077
Methods Mol Biol. 2009;578:437-51
pubmed: 19768611
Pharmacogenomics. 2009 Jul;10(7):1187-97
pubmed: 19604094
Emerg Infect Dis. 2021 Jan;27(1):
pubmed: 33327991
J Infect Chemother. 2022 Jan;28(1):120-123
pubmed: 34580006
Hum Mutat. 2010 Feb;31(2):208-17
pubmed: 20052755
Cell Mol Life Sci. 2009 Oct;66(20):3325-36
pubmed: 19629390