Host glyceraldehyde-3-phosphate dehydrogenase-mediated iron acquisition is hijacked by intraphagosomal Mycobacterium tuberculosis.
Animals
Biological Transport
/ physiology
Cell Line, Tumor
Glyceraldehyde-3-Phosphate Dehydrogenases
/ metabolism
Humans
Iron
/ metabolism
L Cells
Lactoferrin
/ metabolism
Mice
Mice, Inbred C57BL
Mycobacterium tuberculosis
/ metabolism
Phagosomes
/ metabolism
THP-1 Cells
Transferrin
/ metabolism
Tuberculosis
/ pathology
GAPDH
Iron
Lactoferrin
Lipoarabinomannan (LAM)
M.tb
Targeted delivery
Transferrin
Journal
Cellular and molecular life sciences : CMLS
ISSN: 1420-9071
Titre abrégé: Cell Mol Life Sci
Pays: Switzerland
ID NLM: 9705402
Informations de publication
Date de publication:
09 Jan 2022
09 Jan 2022
Historique:
received:
16
09
2021
accepted:
20
12
2021
revised:
08
12
2021
entrez:
10
1
2022
pubmed:
11
1
2022
medline:
19
1
2022
Statut:
epublish
Résumé
Availability of iron is a key factor in the survival and multiplication of Mycobacterium tuberculosis (M.tb) within host macrophage phagosomes. Despite host cell iron regulatory machineries attempts to deny supply of this essential micronutrient, intraphagosomal M.tb continues to access extracellular iron. In the current study, we report that intracellular M.tb exploits mammalian secreted Glyceraldehyde 3-phosphate dehydrogenase (sGAPDH) for the delivery of host iron carrier proteins lactoferrin (Lf) and transferrin (Tf). Studying the trafficking of iron carriers in infected cells we observed that sGAPDH along with the iron carrier proteins are preferentially internalized into infected cells and trafficked to M.tb containing phagosomes where they are internalized by resident mycobacteria resulting in iron delivery. Collectively our findings provide a new mechanism of iron acquisition by M.tb involving the hijack of host sGAPDH. This may contribute to its successful pathogenesis and provide an option for targeted therapeutic intervention.
Identifiants
pubmed: 35001155
doi: 10.1007/s00018-021-04110-3
pii: 10.1007/s00018-021-04110-3
doi:
Substances chimiques
Transferrin
0
Iron
E1UOL152H7
Glyceraldehyde-3-Phosphate Dehydrogenases
EC 1.2.1.-
Lactoferrin
EC 3.4.21.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
62Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Nature Switzerland AG.
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