Phototoxicity induced in living HeLa cells by focused femtosecond laser pulses: a data-driven approach.
Journal
Biomedical optics express
ISSN: 2156-7085
Titre abrégé: Biomed Opt Express
Pays: United States
ID NLM: 101540630
Informations de publication
Date de publication:
01 Dec 2021
01 Dec 2021
Historique:
received:
23
08
2021
revised:
18
11
2021
accepted:
19
11
2021
entrez:
10
1
2022
pubmed:
11
1
2022
medline:
11
1
2022
Statut:
epublish
Résumé
Nonlinear optical microscopy is a powerful label-free imaging technology, providing biochemical and structural information in living cells and tissues. A possible drawback is photodamage induced by high-power ultrashort laser pulses. Here we present an experimental study on thousands of HeLa cells, to characterize the damage induced by focused femtosecond near-infrared laser pulses as a function of laser power, scanning speed and exposure time, in both wide-field and point-scanning illumination configurations. Our data-driven approach offers an interpretation of the underlying damage mechanisms and provides a predictive model that estimates its probability and extension and a safety limit for the working conditions in nonlinear optical microscopy. In particular, we demonstrate that cells can withstand high temperatures for a short amount of time, while they die if exposed for longer times to mild temperatures. It is thus better to illuminate the samples with high irradiances: thanks to the nonlinear imaging mechanism, much stronger signals will be generated, enabling fast imaging and thus avoiding sample photodamage.
Identifiants
pubmed: 35003873
doi: 10.1364/BOE.441225
pii: 441225
pmc: PMC8713694
doi:
Types de publication
Journal Article
Langues
eng
Pagination
7886-7905Informations de copyright
© 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.
Déclaration de conflit d'intérêts
The authors declare no conflicts of interest.
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