Intrinsic factor autoantibodies by luminescent immuno-precipitation system in patients with corpus atrophic gastritis.
Atrophic gastritis
Autoimmune gastritis
CAG, corpus atrophic gastritis
Gastric autoantibodies
Hp, Helicobacter pylori
IFA, intrinsic factor autoantibodies
Intrinsic factor antibodies
LIPS
LIPS, luminescent immuno-precipitation system
Luciferase immunoprecipitation system
PA, pernicious anemia
PCA, antibodies against gastric parietal cells
Parietal cell antibodies
Pernicious anemia
Journal
Journal of translational autoimmunity
ISSN: 2589-9090
Titre abrégé: J Transl Autoimmun
Pays: Netherlands
ID NLM: 101759413
Informations de publication
Date de publication:
2021
2021
Historique:
received:
06
08
2021
revised:
28
10
2021
accepted:
29
10
2021
entrez:
10
1
2022
pubmed:
11
1
2022
medline:
11
1
2022
Statut:
epublish
Résumé
Corpus atrophic gastritis (CAG) may lead to intrinsic factor (IF) deficiency and vitamin B Recombinant nanoluciferase-tagged IF secreted from transfected Expi293F cells was used as antigen in an IFA-LIPS assay. IFA IgG were measured in sera from subjects undergoing gastroscopy and biopsy (updated Sydney system) mainly for anemia (57%) or dyspepsia (34%). This cohort comprised 105 patients with histologically-proven-CAG (cases: median age 64 years, 68% females) and 110 subjects with suspected CAG that were histologically negative (controls: median age 67 years, 54% females). Cut-off values were selected by Q-Q-plot analysis (negative: <2.5 arbitrary units). IFA levels were higher in cases than in controls (Mann-Whitney:p < 10 The IFA-LIPS assay discriminated between CAG patients and controls showing a good specificity (95%) at the cost of sensitivity (32%). IFA-positivity occurred independently from anemia and vitamin B
Sections du résumé
BACKGROUND
BACKGROUND
Corpus atrophic gastritis (CAG) may lead to intrinsic factor (IF) deficiency and vitamin B
METHODS
METHODS
Recombinant nanoluciferase-tagged IF secreted from transfected Expi293F cells was used as antigen in an IFA-LIPS assay. IFA IgG were measured in sera from subjects undergoing gastroscopy and biopsy (updated Sydney system) mainly for anemia (57%) or dyspepsia (34%). This cohort comprised 105 patients with histologically-proven-CAG (cases: median age 64 years, 68% females) and 110 subjects with suspected CAG that were histologically negative (controls: median age 67 years, 54% females). Cut-off values were selected by Q-Q-plot analysis (negative: <2.5 arbitrary units).
RESULTS
RESULTS
IFA levels were higher in cases than in controls (Mann-Whitney:p < 10
CONCLUSIONS
CONCLUSIONS
The IFA-LIPS assay discriminated between CAG patients and controls showing a good specificity (95%) at the cost of sensitivity (32%). IFA-positivity occurred independently from anemia and vitamin B
Identifiants
pubmed: 35005595
doi: 10.1016/j.jtauto.2021.100131
pii: S2589-9090(21)00051-4
pmc: PMC8716657
doi:
Types de publication
Journal Article
Langues
eng
Pagination
100131Informations de copyright
© 2021 The Authors.
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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