Studying macrophage activation in immune-privileged lens through CSF-1 protein intravitreal injection in mouse model.
Immunology
Microscopy
Model Organisms
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
18 03 2022
18 03 2022
Historique:
entrez:
10
1
2022
pubmed:
11
1
2022
medline:
9
4
2022
Statut:
epublish
Résumé
Macrophage (MΦ) activation and promotion of fibrosis are critical processes in lens capsule healing after injury. Here, we detail a protocol that induces MΦ2 formation within the vitreous body of the eye. Our procedure combines the use of an intravitreal injection of a growth factor (CSF-1) and immunofluorescence to confirm the presence of MΦ2 and fibrotic tissue formation. This protocol allows assessment of the distribution of macrophages and quantification of fibrotic tissue formation/sealing within the vitreous body of mouse eyes. For complete details on the use and execution of this profile, please refer to Li et al. (2021), Gerhardt et al. (2003), Kubota et al. (2009).
Identifiants
pubmed: 35005642
doi: 10.1016/j.xpro.2021.101060
pii: S2666-1667(21)00766-8
pmc: PMC8715327
doi:
Substances chimiques
Macrophage Colony-Stimulating Factor
81627-83-0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101060Subventions
Organisme : NEI NIH HHS
ID : R01 EY012085
Pays : United States
Organisme : NCI NIH HHS
ID : T32 CA148724
Pays : United States
Informations de copyright
© 2021 The Author(s).
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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