Severe gastroenteropathy associated with Clostridium perfringens isolation in starving juvenile sturgeons.
C. perfringens
dysmicrobism
gastroenteropathy
starvation
sturgeon
toxin
Journal
Journal of fish diseases
ISSN: 1365-2761
Titre abrégé: J Fish Dis
Pays: England
ID NLM: 9881188
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
revised:
16
12
2021
received:
26
10
2021
accepted:
20
12
2021
pubmed:
11
1
2022
medline:
11
2
2022
entrez:
10
1
2022
Statut:
ppublish
Résumé
In November 2020 a mortality episode (30%) in juvenile Siberian and Russian sturgeons (Acipenser baerii, Brandt, and A. gueldenstaedtii, Brandt & Ratzeburg) and GUBA hybrid sturgeons (A. gueldenstaedtii × A. baerii) occurred in a hatchery in Northern Italy, associated with severe coelomic distension and abnormal reverse surface swimming. The fish were reared in concrete tanks supplied by well water, fed at 0.4% of body weight (b.w.) per day. Thirty sturgeon specimens were collected for necropsy, histological, bacteriological and virological examination. Macroscopic findings included diffuse and severe bloating of gastrointestinal tracts due to foamy contents with thinning and stretching of the gastrointestinal walls. Histological analysis revealed variable degrees of sloughing and necrosis of the intestinal epithelium, and the presence of bacterial aggregates. Anaerobic Gram-positive bacteria were investigated, and Clostridium perfringens was isolated from the gut. Specific PCRs identified the toxinotype A and the β2 toxin gene. The daily feed administration was increased to 1.5% b.w. and after 5 days, the mortality ceased. A new animal cohort from the same groups was examined after 12 weeks, showing neither gut alterations nor isolation of C. perfringens. The imbalance of intestinal microbiota, presumably caused by underfeeding, favoured C. perfringens overgrowth and severe gas formation. The diet increase possibly restored the normal microbiota.
Types de publication
Case Reports
Langues
eng
Sous-ensembles de citation
IM
Pagination
471-477Informations de copyright
© 2022 John Wiley & Sons Ltd.
Références
Allaart, J. G., van Asten, A. J., & Gröne, A. (2013). Predisposing factors and prevention of Clostridium perfringens-associated enteritis. Comparative Immunology, Microbiology & Infectious Diseases, 36, 449-464. https://doi.org/10.1016/j.cimid.2013.05.001
Baums, C. G., Schotte, U., Amtsberg, G., & Goethe, R. (2004). Diagnostic multiplex PCR for toxin genotyping of Clostridium perfringens isolates. Veterinary Microbiology, 100, 11-16. https://doi.org/10.1016/S0378-1135(03)00126-3
Bigarré, L., Lesne, M., Lautraite, A., Chesneau, V., Leroux, A., Jamin, M., Boitard, P. M., Toffan, A., Prearo, M., Labrut, S., & Daniel, P. (2017). Molecular identification of iridoviruses infecting various sturgeon species in Europe. Journal of Fish Diseases, 40, 105-118. https://doi.org/10.1111/jfd.12498
Bronzi, P., Chebanov, M., Michaels, J. T., Wei, Q. W., Rosenthal, H., & Gessner, J. (2019). Sturgeon meat and caviar production: Global update 2017. Journal of Applied Ichthyology, 35, 257-266. https://doi.org/10.1111/jai.13870
Chen, M. H., Hung, S. W., Shyu, C. L., Lin, C. C., Liu, P. C., Chang, C. H., Shia, W. Y., Cheng, C. F., Lin, S. L., Tu, C. Y., Lin, Y. H., & Wang, W. S. (2012). Lactococcus lactis subsp. Lactis infection in Bester sturgeon, a cultured hybrid of Huso huso × Acipenser ruthenus, in Taiwan. Research in Veterinary Science, 93, 581-588. https://doi.org/10.1016/j.rvsc.2011.10.007
Ciulli, S., Volpe, E., Sirri, R., Tura, G., Errani, F., Zamperin, G., Toffan, A., Silvi, M., Renzi, A., Abbadi, M., Biasini, L., Pretto, T., Emmanuele, P., Casalini, A., Sarli, G., Serratore, P., Mordenti, O., & Mandrioli, L. (2020). Multifactorial causes of chronic mortality in juvenile sturgeon (Huso huso). Animals (Basel), 10, 1866. https://doi.org/10.3390/ani10101866
Clouthier, S., Anderson, E., Kurath, G., & Breyta, R. (2018). Molecular systematics of sturgeon nucleocytoplasmic large DNA viruses. Molecular Phylogenetics and Evolution, 128, 26-37. https://doi.org/10.1016/j.ympev.2018.07.019
Di, J., Zhang, S. H., Huang, J., Du, H., Zhou, Y., Zhou, Q., & Wei, Q. W. (2018). Isolation and identification of pathogens causing haemorrhagic septicaemia in cultured Chinese sturgeon (Acipenser sinensis). Aquaculture Research, 49, 3624-3633. https://doi.org/10.1111/are.13830
Doszpoly, A., Kalabekov, I. M., Breyta, R., & Shchelkunov, I. S. (2017). Isolation and characterization of an atypical Siberian sturgeon herpesvirus strain in Russia: Novel North American Acipenserid herpesvirus 2 strain in Europe? Journal of Fish Diseases, 40, 1363-1372. https://doi.org/10.1111/jfd.12611
Drigo, I., Agnoletti, F., Bacchin, C., Bettini, F., Cocchi, M., Ferro, T., Marcon, B., & Bano, L. (2008). Toxin genotyping of Clostridium perfringens field strains isolated from healthy and diseased chickens. Italian Journal of Animal Science, 7, 397-400. https://doi.org/10.4081/ijas.2008.397
Garmory, H. S., Chanter, N., French, N. P., Bueschel, D., Songer, J. G., & Titball, R. W. (2000). Occurrence of Clostridium perfringens beta2-toxin amongst animals, determined using genotyping and subtyping PCR assays. Epidemiology & Infection, 124, 61-67. https://doi.org/10.1017/s0950268899003295. Erratum. In: Epidemiology & Infection (2000) Aug; 125(1):227. PMID: 10722131; PMCID: PMC2810884.
Gholamhosseini, A., Taghadosi, V., Shiry, N., Akhlaghi, M., Sharifiyazdi, H., Soltanian, S., & Ahmadi, N. (2018). First isolation and identification of Aeromonas veronii and Chryseobacterium joostei from reared sturgeons in Fars province, Iran. Veterinary Research Forum, 9, 113-119. https://doi.org/10.30466/VRF.2018.30826
Gisbert, E., & Doroshov, S. I. (2003). Histology of the developing digestive system and the effect of food deprivation in larval green sturgeon (Acipenser medirostris). Aquatic Living Resources, 16, 77-89. https://doi.org/10.1016/S0990-7440(03)00029-9
Gómez, G. D., & Balcázar, J. L. (2008). A review on the interactions between gut microbiota and innate immunity of fish. FEMS Immunology and Medical Microbiology, 52, 145-154. https://doi.org/10.1111/j.1574-695X.2007.00343.x
Huang, X., Zhong, L., Fan, W., Feng, Y., Xiong, G., Liu, S., Wang, K., Geng, Y., Ouyang, P., Chen, D., Yang, S., Yin, L., & Ji, L. (2020). Enteritis in hybrid sturgeon (Acipenser schrenckii♂ × Acipenser baeri♀) caused by intestinal microbiota disorder. Aquaculture Reports, 18, https://doi.org/10.1016/j.aqrep.2020.100456
Józefiak, A., Nogales-Mérida, S., Rawski, M., Kierończyk, B., & Mazurkiewicz, J. (2019). Effects of insect diets on the gastrointestinal tract health and growth performance of Siberian sturgeon (Acipenser baerii Brandt, 1869). BMC Veterinary Research, 15, 348. https://doi.org/10.1186/s12917-019-2070-y
Keyburn, A. L., Boyce, J. D., Vaz, P., Bannam, T. L., Ford, M. E., Parker, D., Di Rubbo, A., Rood, J. I., & Moore, R. J. (2008). NetB, a new toxin that is associated with avian necrotic enteritis caused by Clostridium perfringens. PLoS Path, 4, e26. https://doi.org/10.1371/journal.ppat.0040026
Ma, Z. H., Yang, H., Li, T. L., Luo, L., & Gao, J. L. (2009). Isolation and identification of pathogenic Aeromonas veronii isolated from infected Siberian sturgeon (Acipenser baerii). Acta Microbiologica Sinica, 49, 1289-1294. https://doi.org/10.1016/S1003-6326(09)60084-4
Pérez, T., Alba, C., Aparicio, M., de Andrés, J., Ruiz Santa Quiteria, J. A., Rodríguez, J. M., & Gibello, A. (2019). Abundant bacteria in the proximal and distal intestine of healthy Siberian sturgeons (Acipenser baerii). Aquaculture, 506, 325-336. https://doi.org/10.1016/j.aquaculture.2019.03.055
Radosavljević, V., Milićević, V., Maksimović-Zorić, J., Veljović, L., Nešić, K., Pavlović, M., Ljubojević-Pelić, D., & Marković, Z. (2019). Sturgeon diseases in aquaculture. Archives of Veterinary Medicine, 12, 5-20. https://doi.org/10.46784/e-avm.v12i1.34
Ramos, C. P., Santana, J. A., Morcatti Coura, F., Xavier, R. G. C., Leal, C. A. G., Oliveira Junior, C. A., Heinemann, M. B., Lage, A. P., Lobato, F. C. F., & Silva, R. O. S. (2019). Identification and characterization of Escherichia coli, Salmonella Spp., Clostridium perfringens, and C. difficile isolates from reptiles in Brazil. BioMed Research International, 2019, 9530732. https://doi.org/10.1155/2019/9530732
Rood, J. I., Adams, V., Lacey, J., Lyras, D., McClane, B. A., Melville, S. B., Moore, R. J., Popoff, M. R., Sarker, M. R., Songer, J. G., Uzal, F. A., & Van Immerseel, F. (2018). Expansion of the Clostridium perfringens toxin-based typing scheme. Anaerobe, 53, 5-10. https://doi.org/10.1016/j.anaerobe.2018.04.011
Sabry M., Abd El-Moein K., Hamza E., Abdel Kader F. (2016). Occurrence of Clostridium perfringens Types A, E, and C in Fresh Fish and Its Public Health Significance. Journal of Food Protection, 79(6), 994-1000. http://doi.org/10.4315/0362-028x.jfp-15-569
Santi, M., Pastorino, P., Foglini, C., Righetti, M., Pedron, C., & Prearo, M. (2019). A survey of bacterial infections in sturgeon farming in Italy. Journal of Applied Ichthyology, 35, 275-282. https://doi.org/10.1111/jai.13802
Stachnik, M., Matras, M., Borzym, E., Maj-Paluch, J., & Reichert, M. (2021). Emerging viral pathogens in sturgeon aquaculture in Poland: Focus on Herpesviruses and Mimivirus detection. Viruses, 13, 1496. https://doi.org/10.3390/v13081496
The European Market Observatory for Fisheries and Aquaculture Products - EUMOFA. (2021). The caviar market: production, trade, and consumption in and outside the EU. Retrieved October 12 2021. https://www.eumofa.eu/documents/20178/84590/The+caviar+market_EU.pdf
Uzal, F. A., Vidal, J. E., McClane, B. A., & Gurjar, A. A. (2010). Clostridium perfringens toxins involved in mammalian veterinary diseases. The Open Toxinology Journal, 2, 24-42.
Wang, R., Pan, X., & Xu, Y. (2020). Altered intestinal microbiota composition associated with enteritis in Yellow Seahorses Hippocampus kuda (Bleeker, 1852). Current Microbiology, 77, 730-737. https://doi.org/10.1007/s00284-019-01859-6
Weese, J. S., & Staempfli, H. R. (2000). Diarrhea associated with enterotoxigenic Clostridium perfringens in a red-footed tortoise (Geochelone carbonaria). Journal of Zoo and Wildlife Medicine, 31, 265-266. https://doi.org/10.1638/1042-7260(2000)031[0265:DAWECP]2.0.CO;2
Wu, X., Teame, T., Hao, Q., Ding, Q., Liu, H., Ran, C., Yang, Y., Zhang, Y., Zhou, Z., Duan, M., & Zhang, Z. (2020). Use of a paraprobiotic and postbiotic feed supplement (HWF™) improves the growth performance, composition and function of gut microbiota in hybrid sturgeon (Acipenser baerii x Acipenser schrenckii). Fish and Shellfish Immunology, 104, 36-45. https://doi.org/10.1016/j.fsi.2020.05.054
Zarantoniello, M., Randazzo, B., Nozzi, V., Truzzi, C., Giorgini, E., Cardinaletti, G., Freddi, L., Ratti, S., Girolametti, F., Osimani, A., Notarstefano, V., Milanović, V., Riolo, P., Isidoro, N., Tulli, F., Gioacchini, G., & Olivotto, I. (2021). Physiological responses of Siberian sturgeon (Acipenser baerii) juveniles fed on full-fat insect-based diet in an aquaponic system. Scientific Reports, 11, 1057. https://doi.org/10.1038/s41598-020-80379-x
Zheng, L. P., Geng, Y., Lei, X. P., Yu, Z. H., Huang, X. L., Chen, D. F., Ouyang, P., Cao, S. Q., & Han, R. (2018). Isolation and identification of Streptococcus iniae from sturgeon and its pathological lesions of infection. Acta Agriculturae Zhejiangensis, 30, 203-210. https://doi.org/10.3969/j.issn.1004-1524.2018.02.04