CREG1 improves diet-induced obesity via uncoupling protein 1-dependent manner in mice.
CREG1
UCP1
adipogenesis
obesity
thermoneutrality
Journal
Genes to cells : devoted to molecular & cellular mechanisms
ISSN: 1365-2443
Titre abrégé: Genes Cells
Pays: England
ID NLM: 9607379
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
revised:
06
01
2022
received:
28
10
2021
accepted:
07
01
2022
pubmed:
11
1
2022
medline:
12
3
2022
entrez:
10
1
2022
Statut:
ppublish
Résumé
Thermogenic brown and beige adipocytes express uncoupling protein 1 (UCP1) and stimulate energy metabolism, protecting against obesity and metabolic diseases such as type 2 diabetes and hyperlipidemia. Cellular repressor of E1A-stimulated genes 1 (CREG1) can stimulate thermogenic fat formation, induce UCP1, and reduce diet-induced obesity (DIO) in mice at normal room temperature. In this study, we investigated the effect of CREG1 administration and the importance of UCP1 in DIO inhibition under thermoneutral conditions at 30°C, which attenuate thermogenic fat formation. Interestingly, subcutaneous administration of recombinant CREG1 protein via an osmotic pump in C57BL/6J mice for four weeks increased UCP1 expression in interscapular brown adipose tissue (IBAT), inhibited visceral white fat hypertrophy with partial browning, and reduced DIO compared to that in PBS-treated mice. The mRNA expression of energy metabolism-related genes was significantly increased in the IBAT of CREG1-treated mice compared to that in PBS-treated mice. In contrast, adipocyte-specific overexpression of CREG1 failed to improve DIO in UCP1-knockout mice at thermoneutrality. Our results indicate the therapeutic potential of CREG1 administration for obesity under thermogenic fat-attenuating conditions and highlight the indispensable role of UCP1 in the DIO-inhibitory effect of CREG1.
Substances chimiques
Uncoupling Protein 1
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
202-213Subventions
Organisme : the MEXT-Supported program for Strategic Research Foundation at private universities
ID : S1201007
Organisme : Grant-in-Aid for Scientific Research (KAKENHI) from the Japan Society for the Promotion of Science
ID : 15K07846
Organisme : Grant-in-Aid for Scientific Research (KAKENHI) from the Japan Society for the Promotion of Science
ID : 20K06450
Organisme : Grant from DAIICHI SANKYO HEALTHCARE CO., LTD
ID : TaNeDS2014
Informations de copyright
© 2022 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.
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