The Non-Erythropoietic EPO Analogue Cibinetide Inhibits Osteoclastogenesis In Vitro and Increases Bone Mineral Density in Mice.
CD131
EPOR
bone marrow derived macrophages (BMDM)
cibinetide
erythropoietin
osteoclasts
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
21 Dec 2021
21 Dec 2021
Historique:
received:
22
11
2021
revised:
17
12
2021
accepted:
18
12
2021
entrez:
11
1
2022
pubmed:
12
1
2022
medline:
29
1
2022
Statut:
epublish
Résumé
The two erythropoietin (EPO) receptor forms mediate different cellular responses to erythropoietin. While hematopoiesis is mediated via the homodimeric EPO receptor (EPOR), tissue protection is conferred via a heteromer composed of EPOR and CD131. In the skeletal system, EPO stimulates osteoclast precursors and induces bone loss. However, the underlying molecular mechanisms are still elusive. Here, we evaluated the role of the heteromeric complex in bone metabolism in vivo and in vitro by using Cibinetide (CIB), a non-erythropoietic EPO analogue that exclusively binds the heteromeric receptor. CIB is administered either alone or in combination with EPO. One month of CIB treatment significantly increased the cortical (~5.8%) and trabecular (~5.2%) bone mineral density in C57BL/6J WT female mice. Similarly, administration of CIB for five consecutive days to female mice that concurrently received EPO on days one and four, reduced the number of osteoclast progenitors, defined by flow cytometry as Lin
Identifiants
pubmed: 35008482
pii: ijms23010055
doi: 10.3390/ijms23010055
pmc: PMC8744753
pii:
doi:
Substances chimiques
Epo protein, mouse
0
Oligopeptides
0
Erythropoietin
11096-26-7
cibinetide
9W5677JKDA
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Israel Science Foundation
ID : 1086/17
Organisme : Israel Science Foundation
ID : 1188/21
Organisme : Dotan Hemato-oncology Fund
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