Graphene Enhances Actin Filament Assembly Kinetics and Modulates NIH-3T3 Fibroblast Cell Spreading.
actin cytoskeleton
assembly kinetics
cell spreading
excluded area
graphene
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
03 Jan 2022
03 Jan 2022
Historique:
received:
18
11
2021
revised:
28
12
2021
accepted:
30
12
2021
entrez:
11
1
2022
pubmed:
12
1
2022
medline:
15
2
2022
Statut:
epublish
Résumé
Actin plays critical roles in various cellular functions, including cell morphogenesis, differentiation, and movement. The assembly of actin monomers into double-helical filaments is regulated in surrounding microenvironments. Graphene is an attractive nanomaterial that has been used in various biomaterial applications, such as drug delivery cargo and scaffold for cells, due to its unique physical and chemical properties. Although several studies have shown the potential effects of graphene on actin at the cellular level, the direct influence of graphene on actin filament dynamics has not been studied. Here, we investigate the effects of graphene on actin assembly kinetics using spectroscopy and total internal reflection fluorescence microscopy. We demonstrate that graphene enhances the rates of actin filament growth in a concentration-dependent manner. Furthermore, cell morphology and spreading are modulated in mouse embryo fibroblast NIH-3T3 cultured on a graphene surface without significantly affecting cell viability. Taken together, these results suggest that graphene may have a direct impact on actin cytoskeleton remodeling.
Identifiants
pubmed: 35008935
pii: ijms23010509
doi: 10.3390/ijms23010509
pmc: PMC8745492
pii:
doi:
Substances chimiques
Graphite
7782-42-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIAID NIH HHS
ID : R01 AI139242
Pays : United States
Organisme : National Science Foundation
ID : 1943266
Organisme : NIH HHS
ID : R01AI139242
Pays : United States
Organisme : National Science Foundation
ID : ECCS-1845331
Références
Nanoscale. 2011 Jun;3(6):2461-4
pubmed: 21562671
J Biol Chem. 1971 Aug 10;246(15):4866-71
pubmed: 4254541
Physiol Rev. 2014 Jan;94(1):235-63
pubmed: 24382887
ACS Nano. 2010 Nov 23;4(11):6587-98
pubmed: 20979372
Annu Rev Biophys Biomol Struct. 2000;29:545-76
pubmed: 10940259
J Chem Phys. 2019 May 14;150(18):184906
pubmed: 31091902
Biochem Biophys Res Commun. 2008 May 2;369(2):303-7
pubmed: 18267105
Science. 2009 Nov 27;326(5957):1208-12
pubmed: 19965462
Sci Rep. 2017 Feb 20;7:42767
pubmed: 28218295
Chem Res Toxicol. 2012 Jan 13;25(1):15-34
pubmed: 21954945
Biophys J. 2005 Feb;88(2):1387-402
pubmed: 15556992
Curr Drug Metab. 2015;16(5):397-409
pubmed: 25429670
Int J Pharm. 2017 Feb 25;518(1-2):185-192
pubmed: 28057464
J Biomater Appl. 2011 Sep;26(3):327-47
pubmed: 20566655
Sci Rep. 2016 Dec 07;6:38675
pubmed: 27924860
Adv Mater. 2013 Jan 11;25(2):168-86
pubmed: 23161646
Biomaterials. 2014 Feb;35(5):1597-607
pubmed: 24290441
Biomaterials. 2009 Oct;30(29):5433-44
pubmed: 19595452
Biosens Bioelectron. 2020 Oct 15;166:112471
pubmed: 32777726
Research (Wash D C). 2018 Sep 5;2018:9712832
pubmed: 31549040
Curr Opin Struct Biol. 2000 Feb;10(1):34-9
pubmed: 10679465
Annu Rev Biophys. 2011;40:169-86
pubmed: 21314430
PLoS One. 2014 Apr 16;9(4):e94766
pubmed: 24740323
Int J Biol Macromol. 2016 May;86:546-55
pubmed: 26851208
J Phys Chem B. 2019 Apr 4;123(13):2770-2779
pubmed: 30817154
J Mol Biol. 2008 May 2;378(3):540-50
pubmed: 18374941
Cold Spring Harb Perspect Biol. 2016 Aug 01;8(8):
pubmed: 26988969
Biomaterials. 2011 May;32(15):3700-11
pubmed: 21396708
Micromachines (Basel). 2020 Jan 03;11(1):
pubmed: 31947894
Toxicol In Vitro. 2018 Apr;48:276-285
pubmed: 29409908
Nano Lett. 2010 Nov 10;10(11):4285-94
pubmed: 20879723
Sci Rep. 2021 Aug 10;11(1):16216
pubmed: 34376720
Proc Natl Acad Sci U S A. 2014 Dec 16;111(50):17821-6
pubmed: 25468977
Nature. 2010 Jan 28;463(7280):485-92
pubmed: 20110992
Biotechnol Prog. 2012 Jul;28(4):1069-78
pubmed: 22619183
Biomaterials. 2012 Oct;33(29):6933-42
pubmed: 22796167
ACS Appl Mater Interfaces. 2011 Jul;3(7):2607-15
pubmed: 21650218
J Biol Chem. 2001 Apr 6;276(14):10577-80
pubmed: 11279227
Langmuir. 2018 Dec 18;34(50):15109-15125
pubmed: 30032622
Chemphyschem. 2015 May 18;16(7):1379-85
pubmed: 25704394
Small. 2017 Jan;13(3):
pubmed: 27762498
Colloids Surf B Biointerfaces. 2016 Oct 1;146:785-93
pubmed: 27451366
ACS Nano. 2010 Aug 24;4(8):4872-8
pubmed: 20669976
Materials (Basel). 2021 Jan 30;14(3):
pubmed: 33573304
Methods Mol Biol. 2016;1407:287-306
pubmed: 27271910
Science. 1991 Nov 29;254(5036):1312-9
pubmed: 1962191
J Nanosci Nanotechnol. 2016 Mar;16(3):2408-17
pubmed: 27455649
Dev Cell. 2016 Jan 25;36(2):201-14
pubmed: 26812019
Proc Natl Acad Sci U S A. 2012 Oct 16;109(42):16923-7
pubmed: 23027950
J Phys Chem B. 2018 Apr 12;122(14):3826-3835
pubmed: 29608304
Curr Biol. 2007 May 15;17(10):R357-8
pubmed: 17502086
Nanoscale. 2016 Dec 1;8(47):19491-19509
pubmed: 27878179
Sci Rep. 2016 Sep 22;6:33835
pubmed: 27652886
J Funct Biomater. 2012 May 23;3(2):398-417
pubmed: 24955540
Science. 2004 Apr 30;304(5671):743-6
pubmed: 15118165
Int J Mol Sci. 2018 Nov 12;19(11):
pubmed: 30424535
Science. 1987 Oct 30;238(4827):638-44
pubmed: 3672117
Adv Mater. 2020 Aug;32(34):e2000608
pubmed: 32672882
Biochem Biophys Res Commun. 2020 Nov 19;532(4):548-554
pubmed: 32900483
Adv Mater. 2010 Sep 15;22(35):3906-24
pubmed: 20706983
Int J Mol Sci. 2014 Dec 12;15(12):23090-140
pubmed: 25514413
Proc Natl Acad Sci U S A. 2018 Oct 9;115(41):10345-10350
pubmed: 30254171
ACS Nano. 2012 Apr 24;6(4):3224-9
pubmed: 22390298
Phys Biol. 2015 Apr 30;12(3):034001
pubmed: 25927668
Biochem Biophys Res Commun. 2014 Sep 12;452(1):174-80
pubmed: 25152405