Detection of SARS-CoV-2 RNA in Urine by RT-LAMP: A Very Rare Finding.
COVID-19
RT-LAMP
RT-qPCR
SARS-CoV-2
molecular diagnostics
urine
Journal
Journal of clinical medicine
ISSN: 2077-0383
Titre abrégé: J Clin Med
Pays: Switzerland
ID NLM: 101606588
Informations de publication
Date de publication:
29 Dec 2021
29 Dec 2021
Historique:
received:
17
12
2021
accepted:
27
12
2021
entrez:
11
1
2022
pubmed:
12
1
2022
medline:
12
1
2022
Statut:
epublish
Résumé
Detection of SARS-CoV-2 is routinely performed in naso/oropharyngeal swabs samples from patients via RT-qPCR. The RT-LAMP technology has also been used for viral RNA detection in respiratory specimens with both high sensitivity and specificity. Recently, we developed a novel RT-LAMP test for SARS-CoV-2 RNA detection in nasopharyngeal swab specimens (named, N15-RT-LAMP) that can be performed as a single-tube colorimetric method, in a real-time platform, and as dry-LAMP. To date, there has been very little success in detecting SARS-CoV-2 RNA in urine by RT-qPCR, and the information regarding urine viral excretion is still scarce and not comprehensive. Here, we tested our N15-RT-LAMP on the urine of 300 patients admitted to the Hospital of Salamanca, Spain with clinical suspicion of COVID-19, who had a nasopharyngeal swab RT-qPCR-positive (
Identifiants
pubmed: 35011899
pii: jcm11010158
doi: 10.3390/jcm11010158
pmc: PMC8745354
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Consejería de Educación de La Junta de Castilla y León.European Union cofinancing by FEDER (Fondo Europeo de Desarrollo Regional) 'Una manera de hacer Europa'.Predoctoral Fellowship Program of University of Salamanca and cofinancing by Santander Bank.
ID : COV20EDU/00657
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