The immune-suppressive landscape in lepromatous leprosy revealed by single-cell RNA sequencing.
Journal
Cell discovery
ISSN: 2056-5968
Titre abrégé: Cell Discov
Pays: England
ID NLM: 101661034
Informations de publication
Date de publication:
11 Jan 2022
11 Jan 2022
Historique:
received:
12
03
2021
accepted:
07
11
2021
entrez:
11
1
2022
pubmed:
12
1
2022
medline:
12
1
2022
Statut:
epublish
Résumé
Lepromatous leprosy (L-LEP), caused by the massive proliferation of Mycobacterium leprae primarily in macrophages, is an ideal disease model for investigating the molecular mechanism of intracellular bacteria evading or modulating host immune response. Here, we performed single-cell RNA sequencing of both skin biopsies and peripheral blood mononuclear cells (PBMCs) of L-LEP patients and healthy controls. In L-LEP lesions, we revealed remarkable upregulation of APOE expression that showed a negative correlation with the major histocompatibility complex II gene HLA-DQB2 and MIF, which encodes a pro-inflammatory and anti-microbial cytokine, in the subset of macrophages exhibiting a high expression level of LIPA. The exhaustion of CD8
Identifiants
pubmed: 35013182
doi: 10.1038/s41421-021-00353-3
pii: 10.1038/s41421-021-00353-3
pmc: PMC8748782
doi:
Types de publication
Journal Article
Langues
eng
Pagination
2Subventions
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 81874244
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 81822038
Informations de copyright
© 2021. The Author(s).
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