A Method for in Vivo Quantification Of Cytokine IL-1β In The Rat Intrathecal Space.

IL-1β cytokine sensing longitudinal experiments rat intrathecal space spinal cord

Journal

ACS applied bio materials
ISSN: 2576-6422
Titre abrégé: ACS Appl Bio Mater
Pays: United States
ID NLM: 101729147

Informations de publication

Date de publication:
21 Jan 2020
Historique:
entrez: 12 1 2022
pubmed: 21 1 2020
medline: 21 1 2020
Statut: ppublish

Résumé

IL-1β is a potent pro-inflammatory cytokine critical to multiple pathologies in the central nervous system (CNS). Quantification of IL-1β in vivo is challenging due to pM range of IL-1β released in the spinal cord and also the terminal nature of cerebrospinal fluid (CSF) sampling in rodents. Herein we developed a robust in vivo device on stainless steel suitable for detection of IL-1β in the spinal cord of rats. This approach offers high sensitivity (3.2 pg mL

Identifiants

DOI: 10.1021/acsabm.9b00958 PMID: 35019397
pubmed: 35019397
doi: 10.1021/acsabm.9b00958
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

539-546

Auteurs

Fei Deng (F)

ARC Centre of Excellence in Nanoscale Biophotonics, University of New South Wales, Sydney, New South Wales 2052, Australia.
Australian Centre for NanoMedicine, University of New South Wales, Sydney, New South Wales 2052, Australia.
ORCID: 0000-0002-5606-0278

Azim Arman (A)

ARC Centre of Excellence for Nanoscale Biophotonics, The University of Adelaide, Adelaide, South Australia 5005, Australia.
Institute for Photonics and Advanced Sensing (IPAS) and Adelaide Medical School, The University of Adelaide, Adelaide, South Australia 5005, Australia.

Ewa M Goldys (EM)

ARC Centre of Excellence in Nanoscale Biophotonics, University of New South Wales, Sydney, New South Wales 2052, Australia.
Australian Centre for NanoMedicine, University of New South Wales, Sydney, New South Wales 2052, Australia.

Mark R Hutchinson (MR)

ARC Centre of Excellence for Nanoscale Biophotonics, The University of Adelaide, Adelaide, South Australia 5005, Australia.
Institute for Photonics and Advanced Sensing (IPAS) and Adelaide Medical School, The University of Adelaide, Adelaide, South Australia 5005, Australia.

Guozhen Liu (G)

ARC Centre of Excellence in Nanoscale Biophotonics, University of New South Wales, Sydney, New South Wales 2052, Australia.
Australian Centre for NanoMedicine, University of New South Wales, Sydney, New South Wales 2052, Australia.
ORCID: 0000-0002-0556-6404

Classifications MeSH