High Mycobacterium tuberculosis Bacillary Loads Detected by Tuberculosis Molecular Bacterial Load Assay in Patient Stool: a Potential Alternative for Nonsputum Diagnosis and Treatment Response Monitoring of Tuberculosis.
Mycobacterium tuberculosis
molecular bacterial load assay
molecular diagnostics
Journal
Microbiology spectrum
ISSN: 2165-0497
Titre abrégé: Microbiol Spectr
Pays: United States
ID NLM: 101634614
Informations de publication
Date de publication:
23 02 2022
23 02 2022
Historique:
pubmed:
13
1
2022
medline:
15
3
2022
entrez:
12
1
2022
Statut:
ppublish
Résumé
Not all patients produce sputum, yet most available TB tests use sputum. We investigated the utility of a novel RNA-based quantitative test, the tuberculosis molecular bacterial load assay (TB-MBLA), for the detection and quantification of Mycobacterium tuberculosis in stool. Stools from 100 adult individuals were treated with OMNIgene-sputum reagent and tested using Xpert MTB/RIF ultra (Xpert ultra), auramine O smear microscopy (smear), mycobacterial growth indicator tube (MGIT), and Lowenstein-Jensen (LJ) cultures. The remaining portions were frozen at -20°C and later tested by TB-MBLA. MGIT sputum culture was used as a TB confirmatory test and reference for stool tests. Sixty-one of 100 participants were already confirmed TB positive by MGIT sputum culture, 20 (33%) of whom were HIV coinfected. TB-MBLA detected M. tuberculosis in 57/100 stool samples, including 49 already confirmed for TB. The mean bacterial load measured by stool TB-MBLA was 5.67 ± 1.7 log
Identifiants
pubmed: 35019686
doi: 10.1128/spectrum.02100-21
pmc: PMC8754106
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0210021Subventions
Organisme : NHLBI NIH HHS
ID : K24 HL087713
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL128156
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL143998
Pays : United States
Organisme : NHLBI NIH HHS
ID : U01 HL098964
Pays : United States
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