Correlation between metabolomic profile constituents and feline pancreatic lipase immunoreactivity.
cerebrosides
fPLI
feline pancreatitis
sphingolipids
sphingomyelins
untargeted metabolomics
Journal
Journal of veterinary internal medicine
ISSN: 1939-1676
Titre abrégé: J Vet Intern Med
Pays: United States
ID NLM: 8708660
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
revised:
16
12
2021
received:
09
09
2021
accepted:
22
12
2021
pubmed:
14
1
2022
medline:
1
4
2022
entrez:
13
1
2022
Statut:
ppublish
Résumé
Feline pancreatic lipase immunoreactivity (fPLI) is commonly used to diagnose pancreatitis in cats (FP). Untargeted metabolomics has been extensively applied in human and veterinary medicine, but no metabolomic studies regarding FP have been conducted. To identify metabolites significantly associated with increased fPLI. Forty-nine client-owned cats: 11 clinically healthy and 38 with various clinical conditions. Analytical cross-sectional study with convenience sampling. A panel of 630 metabolites belonging to 26 biochemical classes was quantified in plasma using a commercial metabolomic assay. The correlation between plasma metabolite concentrations and serum fPLI was evaluated using Spearman's rank correlation coefficient (R Four hundred and seven of 630 metabolites (64.6%) were quantified in all cats. When controlled for potential confounders only 3 sphingolipids were significantly positively correlated with fPLI: 2 cerebrosides: HexCer(d18:1/24:0); (R Selected sphingolipids are moderately positively correlated with fPLI and appear to have fair to moderate diagnostic accuracy in discriminating between cats with normal and increased fPLI.
Sections du résumé
BACKGROUND
BACKGROUND
Feline pancreatic lipase immunoreactivity (fPLI) is commonly used to diagnose pancreatitis in cats (FP). Untargeted metabolomics has been extensively applied in human and veterinary medicine, but no metabolomic studies regarding FP have been conducted.
OBJECTIVES
OBJECTIVE
To identify metabolites significantly associated with increased fPLI.
ANIMALS
METHODS
Forty-nine client-owned cats: 11 clinically healthy and 38 with various clinical conditions.
METHODS
METHODS
Analytical cross-sectional study with convenience sampling. A panel of 630 metabolites belonging to 26 biochemical classes was quantified in plasma using a commercial metabolomic assay. The correlation between plasma metabolite concentrations and serum fPLI was evaluated using Spearman's rank correlation coefficient (R
RESULTS
RESULTS
Four hundred and seven of 630 metabolites (64.6%) were quantified in all cats. When controlled for potential confounders only 3 sphingolipids were significantly positively correlated with fPLI: 2 cerebrosides: HexCer(d18:1/24:0); (R
CONCLUSIONS AND CLINICAL IMPORTANCE
CONCLUSIONS
Selected sphingolipids are moderately positively correlated with fPLI and appear to have fair to moderate diagnostic accuracy in discriminating between cats with normal and increased fPLI.
Identifiants
pubmed: 35023223
doi: 10.1111/jvim.16349
pmc: PMC8965226
doi:
Substances chimiques
Lipase
EC 3.1.1.3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
473-481Subventions
Organisme : Narodowe Centrum Nauki
ID : 2019/03/X/NZ5/00164
Informations de copyright
© 2022 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.
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