Phenotype, Allele and Genotype Frequency of ABO and Rhesus D Blood Groups of Blood Donors at the North Gondar District Blood Bank, Northwest Ethiopia.
ABO blood group
Ethiopia
RhD
blood donor
Journal
Journal of blood medicine
ISSN: 1179-2736
Titre abrégé: J Blood Med
Pays: New Zealand
ID NLM: 101550884
Informations de publication
Date de publication:
2022
2022
Historique:
received:
14
11
2021
accepted:
23
12
2021
entrez:
13
1
2022
pubmed:
14
1
2022
medline:
14
1
2022
Statut:
epublish
Résumé
Knowledge of the ABO and RhD group distribution is essential for blood banks inventory and assuring quality blood transfusion services. The objective of this study was to determine the frequency of ABO and RhD phenotype, allele, and genotype among blood donors at North Gondar District Blood Bank from 2010 to 2012, Northwest Ethiopia. The data of the current study were obtained from registration logbooks of blood donors registered. The ABO and RhD grouping was done by using commercially available monoclonal antibodies (anti-A, anti-B and anti-D) by slide methods. Results with no agglutination by anti-D antibody were confirmed using anti-human globulin test. Descriptive statistics were analyzed using SPSS version 20. The allele and genotype frequency of the donors was determined by Hardy-Weinberg equilibrium assumption. The difference between the observed and expected frequency was tested by online Chi-square calculator. P-value of <0.05 was considered statistically significant. Among 6471 blood donors, 82.1%, 94.1% and 55.4% were males, replacement donors and in the age group of 21-30 years, respectively. Blood group O (47.04%) and blood group AB (4.81%) were the dominant and least common, respectively. The distribution of the RhD negative blood group was 5.76%. The distribution of A, B and O alleles was 0.1714, 0.1433 and 0.6859, respectively. Moreover, the genotype frequency of AA, AO, BB, BO, AB and OO was 0.0294, 0.2350, 0.0205, 0.1966, 0.0491 and 0.4704, respectively. The genotype frequency of DD, Dd and dd was 0.5774, 0.3649 and 0.0576, respectively. The result showed that there was no statistically significant difference between observed and expected allele and genotype frequency (P-value >0.05). Blood group O and AB were the most and least prevalent, respectively. The allele and genotype frequency of the population was fulfilled the Hardy-Weinberg equilibrium assumption. This finding might be useful for blood transfusion services.
Sections du résumé
BACKGROUND
BACKGROUND
Knowledge of the ABO and RhD group distribution is essential for blood banks inventory and assuring quality blood transfusion services. The objective of this study was to determine the frequency of ABO and RhD phenotype, allele, and genotype among blood donors at North Gondar District Blood Bank from 2010 to 2012, Northwest Ethiopia.
METHODS
METHODS
The data of the current study were obtained from registration logbooks of blood donors registered. The ABO and RhD grouping was done by using commercially available monoclonal antibodies (anti-A, anti-B and anti-D) by slide methods. Results with no agglutination by anti-D antibody were confirmed using anti-human globulin test. Descriptive statistics were analyzed using SPSS version 20. The allele and genotype frequency of the donors was determined by Hardy-Weinberg equilibrium assumption. The difference between the observed and expected frequency was tested by online Chi-square calculator. P-value of <0.05 was considered statistically significant.
RESULTS
RESULTS
Among 6471 blood donors, 82.1%, 94.1% and 55.4% were males, replacement donors and in the age group of 21-30 years, respectively. Blood group O (47.04%) and blood group AB (4.81%) were the dominant and least common, respectively. The distribution of the RhD negative blood group was 5.76%. The distribution of A, B and O alleles was 0.1714, 0.1433 and 0.6859, respectively. Moreover, the genotype frequency of AA, AO, BB, BO, AB and OO was 0.0294, 0.2350, 0.0205, 0.1966, 0.0491 and 0.4704, respectively. The genotype frequency of DD, Dd and dd was 0.5774, 0.3649 and 0.0576, respectively. The result showed that there was no statistically significant difference between observed and expected allele and genotype frequency (P-value >0.05).
CONCLUSION
CONCLUSIONS
Blood group O and AB were the most and least prevalent, respectively. The allele and genotype frequency of the population was fulfilled the Hardy-Weinberg equilibrium assumption. This finding might be useful for blood transfusion services.
Identifiants
pubmed: 35023982
doi: 10.2147/JBM.S346904
pii: 346904
pmc: PMC8747761
doi:
Types de publication
Journal Article
Langues
eng
Pagination
11-19Informations de copyright
© 2022 Woldu et al.
Déclaration de conflit d'intérêts
The authors declare that there have no conflicts of interest.
Références
Ann Trop Med Parasitol. 1985 Feb;79(1):83-8
pubmed: 3920983
J Clin Diagn Res. 2014 Dec;8(12):FC16-9
pubmed: 25653957
Biomed Res Int. 2018 Apr 23;2018:1925619
pubmed: 29850485
Transfus Clin Biol. 2007 Nov;14(5):435-9
pubmed: 18296094
Int J Immunogenet. 2012 Dec;39(6):477-9
pubmed: 22613080
Asian J Transfus Sci. 2014 Jul;8(2):121-5
pubmed: 25161353
Blood Transfus. 2018 Feb;16(2):178-183
pubmed: 27893352
J Matern Fetal Med. 1997 Nov-Dec;6(6):320-3
pubmed: 9438213
J Blood Med. 2020 Oct 13;11:357-361
pubmed: 33117021
J Blood Med. 2020 Jul 14;11:243-249
pubmed: 32765148
J Blood Med. 2021 Jan 28;12:43-51
pubmed: 33536805
Asian J Transfus Sci. 2010 Jul;4(2):116-22
pubmed: 20859512
BMC Res Notes. 2017 Dec 16;10(1):738
pubmed: 29246245
J Family Med Prim Care. 2019 Jul;8(7):2322-2327
pubmed: 31463250
Niger J Med. 2004 Jul-Sep;13(3):263-6
pubmed: 15532229
Blood. 2000 Jan 15;95(2):375-87
pubmed: 10627438
BMC Res Notes. 2016 Dec 21;9(1):513
pubmed: 28003029
Afr J Med Med Sci. 2000 Mar;29(1):23-6
pubmed: 11379462
Matern Health Neonatol Perinatol. 2021 Feb 2;7(1):8
pubmed: 33531050
Transfus Med Hemother. 2017 Aug;44(4):210-216
pubmed: 28924425
BMC Res Notes. 2017 Jul 26;10(1):330
pubmed: 28747227
J Blood Med. 2010;1:143-6
pubmed: 22282694
J Blood Transfus. 2013;2013:797830
pubmed: 24222890
Transfusion. 2002 Oct;42(10):1261-7
pubmed: 12423508
Gene Geogr. 1989 Apr;3(1):7-10
pubmed: 2518630
PLoS One. 2018 Oct 25;13(10):e0206487
pubmed: 30359434
J Blood Transfus. 2016;2016:7949862
pubmed: 27516920
BMC Hematol. 2019 May 15;19:10
pubmed: 31123590
BMC Res Notes. 2019 Nov 6;12(1):729
pubmed: 31694710
Indian J Pediatr. 2003 Dec;70(12):989-92
pubmed: 14719789
Asian J Transfus Sci. 2008 Jan;2(1):3-5
pubmed: 20041069
Int J Immunogenet. 2011 Feb;38(1):1-5
pubmed: 20670333
J Blood Med. 2020 Sep 28;11:327-334
pubmed: 33061730
Folia Med Cracov. 1992;33(1-4):85-92
pubmed: 1343005
Transfusion. 2000 Apr;40(4):477-89
pubmed: 10773062
Blood Transfus. 2014 Apr;12(2):159-65
pubmed: 23245714
Ethiop J Health Sci. 2018 Sep;28(5):571-582
pubmed: 30607072
BMC Health Serv Res. 2019 Jul 22;19(1):510
pubmed: 31331326
Int J Immunogenet. 2014 Jun;41(3):206-10
pubmed: 24628906
East Mediterr Health J. 2000 Jan;6(1):156-8
pubmed: 11370328