Glucose but Not Fructose Alters the Intestinal Paracellular Permeability in Association With Gut Inflammation and Dysbiosis in Mice.
Animals
Caco-2 Cells
Cecum
/ metabolism
Cytokines
/ blood
DNA, Bacterial
/ genetics
Dysbiosis
/ metabolism
Fructose
/ pharmacology
Gastrointestinal Microbiome
/ drug effects
Glucose
/ pharmacology
Humans
Inflammation
/ blood
Intestinal Mucosa
/ drug effects
Male
Mice, Inbred C57BL
Myosin-Light-Chain Kinase
/ metabolism
Permeability
/ drug effects
Caco-2
Desulfovibrio
fructose
glucose
paracellular permeability
pro-inflammatory cytokines
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2021
2021
Historique:
received:
16
07
2021
accepted:
11
10
2021
entrez:
13
1
2022
pubmed:
14
1
2022
medline:
17
2
2022
Statut:
epublish
Résumé
A causal correlation between the metabolic disorders associated with sugar intake and disruption of the gastrointestinal (GI) homeostasis has been suggested, but the underlying mechanisms remain unclear. To unravel these mechanisms, we investigated the effect of physiological amounts of fructose and glucose on barrier functions and inflammatory status in various regions of the GI tract and on the cecal microbiota composition. C57BL/6 mice were fed chow diet and given 15% glucose or 15% fructose in drinking water for 9 weeks. We monitored caloric intake, body weight, glucose intolerance, and adiposity. The intestinal paracellular permeability, cytokine, and tight junction protein expression were assessed in the jejunum, cecum, and colon. In the cecum, the microbiota composition was determined. Glucose-fed mice developed a marked increase in total adiposity, glucose intolerance, and paracellular permeability in the jejunum and cecum while fructose absorption did not affect any of these parameters. Fructose-fed mice displayed increased circulation levels of IL6. In the cecum, both glucose and fructose intake were associated with an increase in
Identifiants
pubmed: 35024040
doi: 10.3389/fimmu.2021.742584
pmc: PMC8744209
doi:
Substances chimiques
Cytokines
0
DNA, Bacterial
0
Fructose
30237-26-4
Myosin-Light-Chain Kinase
EC 2.7.11.18
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
742584Informations de copyright
Copyright © 2021 Zhang, Monnoye, Mariadassou, Beguet-Crespel, Lapaque, Heberden and Douard.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Proc Natl Acad Sci U S A. 2012 Mar 13;109(11):4320-5
pubmed: 22371574
Gut. 2006 May;55(5):630-7
pubmed: 16236773
Physiol Rev. 2010 Jan;90(1):23-46
pubmed: 20086073
Glia. 2015 Feb;63(2):229-41
pubmed: 25161129
Cell Metab. 2018 Feb 6;27(2):351-361.e3
pubmed: 29414685
Proc Natl Acad Sci U S A. 2001 Nov 6;98(23):13306-11
pubmed: 11687633
Appl Environ Microbiol. 2013 Sep;79(17):5112-20
pubmed: 23793624
Pediatr Res. 1995 Jun;37(6):777-82
pubmed: 7651763
J Clin Invest. 2018 Jun 1;128(6):2226-2238
pubmed: 29533924
Gastroenterology. 2016 Oct;151(4):733-746.e12
pubmed: 27342212
Bioinformatics. 2009 Nov 1;25(21):2855-6
pubmed: 19706745
J Cell Biol. 1997 Jun 16;137(6):1393-401
pubmed: 9182670
PeerJ. 2018 Apr 2;6:e4600
pubmed: 29629248
Carcinogenesis. 1997 Jun;18(6):1265-70
pubmed: 9214612
Am J Pathol. 2005 Feb;166(2):409-19
pubmed: 15681825
Can J Physiol Pharmacol. 1993 Oct-Nov;71(10-11):835-9
pubmed: 7511482
Nutrients. 2018 Jun 13;10(6):
pubmed: 29899272
Microbiol Mol Biol Rev. 2008 Dec;72(4):728-64, Table of Contents
pubmed: 19052326
Diabetol Metab Syndr. 2012 Jun 20;4(1):28
pubmed: 22716219
J Nutr. 2017 May;147(5):770-780
pubmed: 28356436
J Clin Invest. 1989 Feb;83(2):724-7
pubmed: 2492310
Am J Physiol Gastrointest Liver Physiol. 2005 Mar;288(3):G422-30
pubmed: 15701621
J Biol Chem. 2006 Sep 8;281(36):26205-15
pubmed: 16835238
Clin Nutr. 2021 Apr;40(4):1691-1698
pubmed: 33413911
Obesity (Silver Spring). 2018 May;26(5):792-800
pubmed: 29687645
Genome Biol. 2014;15(12):550
pubmed: 25516281
Mol Metab. 2016 Nov 04;6(1):61-72
pubmed: 28123938
FEMS Microbiol Ecol. 2002 May 1;40(2):107-12
pubmed: 19709217
Semin Cell Dev Biol. 2014 Dec;36:204-12
pubmed: 25263012
Obesity (Silver Spring). 2015 Jul;23(7):1429-39
pubmed: 26053244
BMJ. 2012 Jan 15;346:e7492
pubmed: 23321486
Science. 2021 Mar 19;371(6535):
pubmed: 33737460
Physiol Behav. 2021 Jan 1;228:113187
pubmed: 32987042
Mol Nutr Food Res. 2016 Apr;60(4):897-908
pubmed: 26679432
Gastroenterology. 2007 Feb;132(2):562-75
pubmed: 17258729
Am J Physiol Endocrinol Metab. 2017 Feb 1;312(2):E109-E116
pubmed: 28028036
Obes Res. 2005 Jul;13(7):1146-56
pubmed: 16076983
Gut. 2004 Feb;53(2):222-8
pubmed: 14724154
Br J Nutr. 2015 Oct 14;114(7):999-1012
pubmed: 26228057
BMC Gastroenterol. 2011 Jan 14;11:3
pubmed: 21235736
Adv Drug Deliv Rev. 2000 Jun 30;41(3):303-13
pubmed: 10854688
Gut. 2003 Jun;52(6):827-33
pubmed: 12740338
Gastroenterology. 2002 Jul;123(1):163-72
pubmed: 12105845
J Cell Biol. 2010 Apr 5;189(1):111-26
pubmed: 20351069
Gastroenterology. 2005 Aug;129(2):550-64
pubmed: 16083712
FEBS Lett. 2015 Jan 2;589(1):165-71
pubmed: 25479086
Am J Clin Nutr. 2014 Jul;100(1):218-32
pubmed: 24787496
Am J Clin Nutr. 1993 Nov;58(5 Suppl):754S-765S
pubmed: 8213607
Proc Natl Acad Sci U S A. 2009 Jan 6;106(1):61-6
pubmed: 19114660
Front Immunol. 2021 Jan 11;11:594150
pubmed: 33505393
Immunity. 2020 Aug 18;53(2):398-416.e8
pubmed: 32814028
J Am Soc Nephrol. 2014 Nov;25(11):2526-38
pubmed: 24876114
Int J Mol Sci. 2019 Dec 03;20(23):
pubmed: 31816903
Mol Nutr Food Res. 2017 Oct;61(10):
pubmed: 28512779
PLoS One. 2013;8(1):e54335
pubmed: 23372709
PLoS Comput Biol. 2017 Nov 3;13(11):e1005752
pubmed: 29099853
Am J Public Health. 2013 Nov;103(11):2071-7
pubmed: 23488503
Gastroenterology. 2014 Dec;147(6):1230-7
pubmed: 25305504
Cell Biol Toxicol. 2005 Jan;21(1):1-26
pubmed: 15868485
Lab Invest. 2012 Jul;92(7):1020-32
pubmed: 22525431
Front Pharmacol. 2021 Feb 22;12:632569
pubmed: 33692694
Am J Physiol Endocrinol Metab. 2008 Aug;295(2):E227-37
pubmed: 18398011
Am J Physiol Cell Physiol. 2000 Dec;279(6):C1918-24
pubmed: 11078707
Am J Physiol Gastrointest Liver Physiol. 2006 Mar;290(3):G496-504
pubmed: 16474009
Lab Invest. 2005 Sep;85(9):1139-62
pubmed: 16007110
Cardiovasc Res. 2010 Jul 15;87(2):272-80
pubmed: 20479130
Eur J Clin Nutr. 2012 Feb;66(2):201-8
pubmed: 21952692
Nat Rev Gastroenterol Hepatol. 2013 Feb;10(2):90-100
pubmed: 23165236
Dig Dis Sci. 2000 Nov;45(11):2122-6
pubmed: 11215725
Obesity (Silver Spring). 2019 Jun;27(6):879-887
pubmed: 31054268
Nat Commun. 2021 Jan 11;12(1):271
pubmed: 33431867
Am J Physiol Gastrointest Liver Physiol. 2008 Apr;294(4):G1060-9
pubmed: 18292183
Am J Physiol Gastrointest Liver Physiol. 2010 Mar;298(3):G335-44
pubmed: 19713474
J Biol Chem. 2010 Apr 16;285(16):12037-46
pubmed: 20177070
FEMS Microbiol Ecol. 2009 Jun;68(3):351-62
pubmed: 19302550
Life Sci. 2017 Nov 15;189:29-38
pubmed: 28912045
Nat Methods. 2013 Jan;10(1):57-9
pubmed: 23202435
Biotechnol Bioeng. 1990 Mar 5;35(5):533-9
pubmed: 18592547
Nature. 2013 Aug 29;500(7464):541-6
pubmed: 23985870
Am J Physiol. 1990 Nov;259(5 Pt 1):G822-37
pubmed: 2240224
Nutrients. 2019 Aug 12;11(8):
pubmed: 31408965
Gastroenterology. 2006 Oct;131(4):1153-63
pubmed: 17030185
Gut. 2004 Apr;53(4):523-9
pubmed: 15016746
Diabetes Care. 2014 Apr;37(4):950-6
pubmed: 24652725
J Nutr Biochem. 2015 Nov;26(11):1183-92
pubmed: 26168700
Am J Clin Nutr. 2008 Dec;88(6):1733S-1737S
pubmed: 19064538
Am J Physiol. 1992 Mar;262(3 Pt 1):C795-800
pubmed: 1550217
Am J Physiol Gastrointest Liver Physiol. 2014 Jan 1;306(1):G1-12
pubmed: 24177030
Trends Neurosci. 2015 Jun;38(6):364-74
pubmed: 25975510
ISME J. 2010 Feb;4(2):232-41
pubmed: 19865183
Gastroenterology. 2000 Oct;119(4):1029-36
pubmed: 11040189
J Exp Med. 2007 Dec 24;204(13):3067-76
pubmed: 18039951
FASEB J. 2019 Jun;33(6):7126-7142
pubmed: 30939042
Gastroenterology. 2017 Oct;153(4):1068-1081.e7
pubmed: 28711628
Cell. 1998 Apr 17;93(2):189-201
pubmed: 9568712
Bioinformatics. 2018 Apr 15;34(8):1287-1294
pubmed: 29228191
Nat Commun. 2021 Feb 22;12(1):1209
pubmed: 33619282
Public Health Nutr. 2014 Mar;17(3):587-96
pubmed: 23414749
Science. 2018 Mar 23;359(6382):1376-1383
pubmed: 29519916
FEMS Microbiol Ecol. 2014 Feb;87(2):357-67
pubmed: 24117923
Hepatology. 2009 Oct;50(4):1094-104
pubmed: 19637282
Am J Clin Nutr. 2016 Aug;104(2):306-14
pubmed: 27357093
Front Microbiol. 2019 Jun 25;10:1423
pubmed: 31293553
Sci Transl Med. 2020 Oct 28;12(567):
pubmed: 33115951