Heterologous expression of four recombinant toxins from Panamanian scorpions of the genus
Animal venom
Centruroides
Proteomics
Recombinant protein
Tityus
Journal
Toxicon: X
ISSN: 2590-1710
Titre abrégé: Toxicon X
Pays: England
ID NLM: 101741983
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
received:
02
09
2021
revised:
13
12
2021
accepted:
15
12
2021
entrez:
13
1
2022
pubmed:
14
1
2022
medline:
14
1
2022
Statut:
epublish
Résumé
The development of more effective antivenoms remains a necessity for countries where scorpionism is a public health problem. Also, the regionalization of antivenoms may be important for some countries with special scorpionism characteristics. Production of antibodies capable of neutralizing the lethal effect of the venom of three scorpion species from Panama. The primary structures of two neurotoxins from The antibodies produced under the reported immunization scheme show better neutralization (ED The information reported here shows the proof of concept for selecting recombinant immunogens with the ability to produce antibodies for neutralizing the lethal effects of the most important medical species of scorpions in Panama.
Sections du résumé
BACKGROUND
BACKGROUND
The development of more effective antivenoms remains a necessity for countries where scorpionism is a public health problem. Also, the regionalization of antivenoms may be important for some countries with special scorpionism characteristics.
OBJECTIVE
OBJECTIVE
Production of antibodies capable of neutralizing the lethal effect of the venom of three scorpion species from Panama.
METHODS
METHODS
The primary structures of two neurotoxins from
RESULTS
RESULTS
The antibodies produced under the reported immunization scheme show better neutralization (ED
CONCLUSION
CONCLUSIONS
The information reported here shows the proof of concept for selecting recombinant immunogens with the ability to produce antibodies for neutralizing the lethal effects of the most important medical species of scorpions in Panama.
Identifiants
pubmed: 35024608
doi: 10.1016/j.toxcx.2021.100090
pii: S2590-1710(21)00026-6
pmc: PMC8728522
doi:
Types de publication
Journal Article
Langues
eng
Pagination
100090Informations de copyright
© 2021 The Author(s).
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Références
Eur J Biochem. 1999 Sep;264(3):746-55
pubmed: 10491120
Immunol Lett. 2009 Aug 15;125(2):93-9
pubmed: 19524619
Toxicon. 2018 Jan;141:79-87
pubmed: 29196122
WHO Offset Publ. 1981;(58):1-44
pubmed: 7245916
Biomedica. 2004 Mar;24(1):42-9
pubmed: 15239600
Trans R Soc Trop Med Hyg. 2004 Dec;98(12):742-50
pubmed: 15485705
Toxins (Basel). 2019 Dec 02;11(12):
pubmed: 31810356
Toxins (Basel). 2017 May 13;9(5):
pubmed: 28505100
Toxicon. 2015 Sep;103:99-105
pubmed: 26100664
Toxicon. 2017 Mar 15;128:5-14
pubmed: 28126552
Acta Trop. 2008 Aug;107(2):71-9
pubmed: 18579104
Biochim Biophys Acta. 2012 Mar;1824(3):478-87
pubmed: 22251893
Toxicon. 2008 Dec 1;52(7):787-93
pubmed: 18775739
Int Immunopharmacol. 2010 Nov;10(11):1318-24
pubmed: 20849955
Protein Expr Purif. 2019 Feb;154:33-43
pubmed: 30205154
Toxicon. 2003 Jun;41(8):971-7
pubmed: 12875871
Acta Trop. 2020 Apr;204:105346
pubmed: 31982434
Nat Commun. 2019 Aug 13;10(1):3642
pubmed: 31409779
Toxicon. 2013 Jul;69:180-90
pubmed: 23506858
Toxicon. 2006 Jul;48(1):64-74
pubmed: 16759681
Toxicon. 1994 Mar;32(3):351-63
pubmed: 8016856
Nucleic Acids Res. 2018 Jul 2;46(W1):W315-W322
pubmed: 29893907
Eur J Biochem. 1999 Sep;264(2):287-300
pubmed: 10491073