Multicentre interlaboratory analysis of routine susceptibility testing with a challenge panel of resistant strains.
Automated susceptibility testing
Disk diffusion
Interlaboratory assay
Multidrug-resistant organisms
Quality control
Journal
Journal of global antimicrobial resistance
ISSN: 2213-7173
Titre abrégé: J Glob Antimicrob Resist
Pays: Netherlands
ID NLM: 101622459
Informations de publication
Date de publication:
03 2022
03 2022
Historique:
received:
19
09
2021
revised:
08
12
2021
accepted:
27
12
2021
pubmed:
14
1
2022
medline:
6
5
2022
entrez:
13
1
2022
Statut:
ppublish
Résumé
In order to elaborate a new national challenge panel of resistant Gram-negative bacilli and Gram-positive cocci strains for the validation of routine antimicrobial susceptibility testing (AST) methods, an interlaboratory evaluation was organised. The results of 12 well-characterised multidrug-resistant strains tested by nine laboratories using local disk diffusion (DD) and automated AST (AUST) methods were compared with the reference broth microdilution method. Overall categorical agreement ranged from 70% to 100% both for DD and AUST and was >90% for all but one strain for all antibiotics. Our multicentre AST study showed good reproducibility and the panel can be used as national resistant reference strains for routine AST validation.
Identifiants
pubmed: 35026463
pii: S2213-7165(22)00005-4
doi: 10.1016/j.jgar.2021.12.020
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Anti-Infective Agents
0
Types de publication
Journal Article
Multicenter Study
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
125-129Informations de copyright
Copyright © 2022 The Author(s). Published by Elsevier Ltd.. All rights reserved.