Tumor necrosis factor-α enhances the expression of vascular endothelial growth factor in a mouse orthodontic tooth movement model.
Bone resorption
Mouse
Orthodontics
Osteoclast
Tooth movement
VEGF
Journal
Journal of dental sciences
ISSN: 2213-8862
Titre abrégé: J Dent Sci
Pays: Netherlands
ID NLM: 101293181
Informations de publication
Date de publication:
Jan 2022
Jan 2022
Historique:
received:
14
07
2021
revised:
19
08
2021
entrez:
14
1
2022
pubmed:
15
1
2022
medline:
15
1
2022
Statut:
ppublish
Résumé
Tooth movement that is achieved using orthodontic mechanical principles relies on bone resorption which takes place on the compression side via osteoclasts. Tumor necrosis factor-α (TNF-α) has been known to affect osteoclast formation in orthodontic tooth movement (OTM). Vascular endothelial growth factor (VEGF), which is one of the mediators of angiogenesis, also plays an important role in OTM by inducing vascular permeability and chemotaxis of osteoclast precursors. Therefore, the purpose of this research was to evaluate the effect of TNF-α on VEGF expression during OTM. In order to demonstrate the effect of TNF-α on VEGF expression during OTM, a nickel titanium closed coil spring was fixed to the upper left first molar and the alveolar bone beneath the upper incisors of both wild type (WT) and TNF receptors (TNFRs) deficient mice resulting in a mesial movement of the molar for 12 days. The maxilla was removed for histological analysis and real-time RCR analysis of VEGF expression. Immunohistochemical analysis revealed that there were fewer VEGF-positive cells in the periodontal membrane on the mesial side of the distobuccal root in TNFRs-deficient mice than that in WT mice during the OTM for 12 days. Furthermore, expression of VEGF mRNA is lower level in TNFRs-deficient mice than that in WT mice. Our results indicate that TNF-α plays an important role in VEGF expression during tooth movement.
Sections du résumé
BACKGROUND/PURPOSE
OBJECTIVE
Tooth movement that is achieved using orthodontic mechanical principles relies on bone resorption which takes place on the compression side via osteoclasts. Tumor necrosis factor-α (TNF-α) has been known to affect osteoclast formation in orthodontic tooth movement (OTM). Vascular endothelial growth factor (VEGF), which is one of the mediators of angiogenesis, also plays an important role in OTM by inducing vascular permeability and chemotaxis of osteoclast precursors. Therefore, the purpose of this research was to evaluate the effect of TNF-α on VEGF expression during OTM.
MATERIALS AND METHODS
METHODS
In order to demonstrate the effect of TNF-α on VEGF expression during OTM, a nickel titanium closed coil spring was fixed to the upper left first molar and the alveolar bone beneath the upper incisors of both wild type (WT) and TNF receptors (TNFRs) deficient mice resulting in a mesial movement of the molar for 12 days. The maxilla was removed for histological analysis and real-time RCR analysis of VEGF expression.
RESULTS
RESULTS
Immunohistochemical analysis revealed that there were fewer VEGF-positive cells in the periodontal membrane on the mesial side of the distobuccal root in TNFRs-deficient mice than that in WT mice during the OTM for 12 days. Furthermore, expression of VEGF mRNA is lower level in TNFRs-deficient mice than that in WT mice.
CONCLUSION
CONCLUSIONS
Our results indicate that TNF-α plays an important role in VEGF expression during tooth movement.
Identifiants
pubmed: 35028065
doi: 10.1016/j.jds.2021.08.011
pii: S1991-7902(21)00202-6
pmc: PMC8739756
doi:
Types de publication
Journal Article
Langues
eng
Pagination
415-420Informations de copyright
© 2021 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V.
Déclaration de conflit d'intérêts
The authors declare that this research was conducted in the absence of any relationships that could be construed as a potential conflict of interest.
Références
J Clin Invest. 2002 Nov;110(10):1419-27
pubmed: 12438440
Arthritis Rheum. 1998 Jun;41(6):951-62
pubmed: 9627005
J Periodontal Res. 2004 Feb;39(1):42-9
pubmed: 14687227
Arch Oral Biol. 2020 Sep;117:104796
pubmed: 32544645
Eur J Orthod. 2009 Dec;31(6):572-7
pubmed: 19840975
Am J Orthod Dentofacial Orthop. 1995 Nov;108(5):519-24
pubmed: 7484971
J Clin Invest. 1997 Sep 15;100(6):1557-65
pubmed: 9294124
J Periodontol. 2007 Mar;78(3):453-8
pubmed: 17335368
Angle Orthod. 2021 Jan 1;91(1):111-118
pubmed: 33289799
J Biol Chem. 1996 Nov 8;271(45):28220-8
pubmed: 8910439
J Biol Chem. 2000 Feb 18;275(7):4858-64
pubmed: 10671521
Eur J Orthod. 2006 Jun;28(3):221-40
pubmed: 16687469
Am J Physiol. 1997 Nov;273(5):H2091-104
pubmed: 9374741
J Dent Res. 2009 Jul;88(7):597-608
pubmed: 19641146
J Exp Med. 2000 Jan 17;191(2):275-86
pubmed: 10637272
Scand J Rheumatol. 1998;27(1):78-9
pubmed: 9506883
Nat Rev Immunol. 2007 Jun;7(6):429-42
pubmed: 17525752
Arterioscler Thromb Vasc Biol. 2009 Jun;29(6):789-91
pubmed: 19164810
J Immunol. 2005 May 1;174(9):5846-55
pubmed: 15843589
Eur J Oral Sci. 2007 Oct;115(5):355-62
pubmed: 17850423
Br J Dermatol. 1998 Dec;139(6):1049-51
pubmed: 9990370
Biol Reprod. 2020 Feb 12;102(1):63-75
pubmed: 31436293
Biomed Res Int. 2020 Aug 18;2020:7189084
pubmed: 32923485
J Dent Res. 2008 Apr;87(4):396-400
pubmed: 18362327
Am J Pathol. 2007 Feb;170(2):427-35
pubmed: 17255310
PLoS One. 2019 Oct 16;14(10):e0223989
pubmed: 31618254
J Biol Chem. 1996 Nov 15;271(46):28890-7
pubmed: 8910536
J Bone Miner Metab. 2006;24(1):20-7
pubmed: 16369894
Am J Pathol. 1999 Jan;154(1):203-10
pubmed: 9916934
J Exp Med. 2005 Sep 5;202(5):589-95
pubmed: 16147974
J Family Med Prim Care. 2019 May;8(5):1602-1606
pubmed: 31198722
Am J Orthod Dentofacial Orthop. 2006 Apr;129(4):458-68
pubmed: 16627170
Clin Exp Rheumatol. 2001 May-Jun;19(3):321-4
pubmed: 11407088
Angle Orthod. 2006 Sep;76(5):830-6
pubmed: 17029518
J Dent Res. 2007 Nov;86(11):1089-94
pubmed: 17959902
Mech Dev. 2002 Feb;111(1-2):61-73
pubmed: 11804779
PLoS One. 2015 Apr 20;10(4):e0124909
pubmed: 25894998