A simple multiplex polymerase chain reaction assay for rapid identification of the common pathogenic dermatophytes: Trichophyton interdigitale, Trichophyton rubrum, and Epidermophyton floccosum.
Dermatophyte
Multiplex PCR
E. floccosum
T. interdigitale/T. mentagrophytes
T. rubrum
Journal
Current medical mycology
ISSN: 2423-3439
Titre abrégé: Curr Med Mycol
Pays: Iran
ID NLM: 101647935
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
received:
20
02
2021
revised:
22
05
2021
accepted:
30
06
2021
entrez:
14
1
2022
pubmed:
15
1
2022
medline:
15
1
2022
Statut:
ppublish
Résumé
The most common etiological agents of human dermatophytosis in various parts of the world are The reliable sequences of several molecular targets of dermatophytes species were used to design a multiplex PCR for the identification of common pathogenic dermatophytes. The isolates and clinical specimens examined in this study included seven standard strains of dermatophytes, 101 isolates of dermatophytes and non-dermatophyte molds/yeasts which had already been identified by sequencing or PCR-restriction fragment length polymorphism (RFLP), and 155 clinical samples from patients suspected of cutaneous mycoses. Species-specific primer pairs for The multiplex assay could detect three common agents out of several causal agents of dermatophytosis, namely
Sections du résumé
BACKGROUND AND PURPOSE
OBJECTIVE
The most common etiological agents of human dermatophytosis in various parts of the world are
MATERIALS AND METHODS
METHODS
The reliable sequences of several molecular targets of dermatophytes species were used to design a multiplex PCR for the identification of common pathogenic dermatophytes. The isolates and clinical specimens examined in this study included seven standard strains of dermatophytes, 101 isolates of dermatophytes and non-dermatophyte molds/yeasts which had already been identified by sequencing or PCR-restriction fragment length polymorphism (RFLP), and 155 clinical samples from patients suspected of cutaneous mycoses.
RESULTS
RESULTS
Species-specific primer pairs for
CONCLUSION
CONCLUSIONS
The multiplex assay could detect three common agents out of several causal agents of dermatophytosis, namely
Identifiants
pubmed: 35028478
doi: 10.18502/cmm.7.2.7030
pii: CMM-7-2
pmc: PMC8740852
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1-7Informations de copyright
Copyright: © 2021, Published by Mazandaran University of Medical Sciences on behalf of Iranian Society of Medical Mycology and Invasive Fungi Research Center.
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