One-carbon metabolism in children with marasmus and kwashiorkor.


Journal

EBioMedicine
ISSN: 2352-3964
Titre abrégé: EBioMedicine
Pays: Netherlands
ID NLM: 101647039

Informations de publication

Date de publication:
Jan 2022
Historique:
received: 05 03 2021
revised: 24 11 2021
accepted: 16 12 2021
pubmed: 15 1 2022
medline: 1 4 2022
entrez: 14 1 2022
Statut: ppublish

Résumé

Kwashiorkor is a childhood syndrome of edematous malnutrition. Its precise nutritional precipitants remain uncertain despite nine decades of study. Remarkably, kwashiorkor's disturbances resemble the effects of experimental diets that are deficient in one-carbon nutrients. This similarity suggests that kwashiorkor may represent a nutritionally mediated syndrome of acute one-carbon metabolism dysfunction. Here we report findings from a cross-sectional exploration of serum one-carbon metabolites in Malawian children. Blood was collected from children aged 12-60 months before nutritional rehabilitation: kwashiorkor (N = 94), marasmic-kwashiorkor (N = 43) marasmus (N = 118), moderate acute malnutrition (N = 56) and controls (N = 46). Serum concentrations of 16 one-carbon metabolites were quantified using LC/MS techniques, and then compared across participant groups. Twelve of 16 measured one-carbon metabolites differed significantly between participant groups. Measured outputs of one-carbon metabolism, asymmetric dimethylarginine (ADMA) and cysteine, were lower in marasmic-kwashiorkor (median µmol/L (± SD): 0·549 (± 0·217) P = 0·00045 & 90 (± 40) P < 0·0001, respectively) and kwashiorkor (0·557 (± 0·195) P < 0·0001 & 115 (± 50) P < 0·0001), relative to marasmus (0·698 (± 0·212) & 153 (± 42)). ADMA and cysteine were well correlated with methionine in both kwashiorkor and marasmic-kwashiorkor. Kwashiorkor and marasmic-kwashiorkor were distinguished by evidence of one-carbon metabolism dysfunction. Correlative observations suggest that methionine deficiency drives this dysfunction, which is implicated in the syndrome's pathogenesis. The hypothesis that kwashiorkor can be prevented by fortifying low quality diets with methionine, along with nutrients that support efficient methionine use, such as choline, requires further investigation. The Hickey Family Foundation, the American College of Gastroenterology, the NICHD, and the USDA/ARS.

Sections du résumé

BACKGROUND BACKGROUND
Kwashiorkor is a childhood syndrome of edematous malnutrition. Its precise nutritional precipitants remain uncertain despite nine decades of study. Remarkably, kwashiorkor's disturbances resemble the effects of experimental diets that are deficient in one-carbon nutrients. This similarity suggests that kwashiorkor may represent a nutritionally mediated syndrome of acute one-carbon metabolism dysfunction. Here we report findings from a cross-sectional exploration of serum one-carbon metabolites in Malawian children.
METHODS METHODS
Blood was collected from children aged 12-60 months before nutritional rehabilitation: kwashiorkor (N = 94), marasmic-kwashiorkor (N = 43) marasmus (N = 118), moderate acute malnutrition (N = 56) and controls (N = 46). Serum concentrations of 16 one-carbon metabolites were quantified using LC/MS techniques, and then compared across participant groups.
FINDINGS RESULTS
Twelve of 16 measured one-carbon metabolites differed significantly between participant groups. Measured outputs of one-carbon metabolism, asymmetric dimethylarginine (ADMA) and cysteine, were lower in marasmic-kwashiorkor (median µmol/L (± SD): 0·549 (± 0·217) P = 0·00045 & 90 (± 40) P < 0·0001, respectively) and kwashiorkor (0·557 (± 0·195) P < 0·0001 & 115 (± 50) P < 0·0001), relative to marasmus (0·698 (± 0·212) & 153 (± 42)). ADMA and cysteine were well correlated with methionine in both kwashiorkor and marasmic-kwashiorkor.
INTERPRETATION CONCLUSIONS
Kwashiorkor and marasmic-kwashiorkor were distinguished by evidence of one-carbon metabolism dysfunction. Correlative observations suggest that methionine deficiency drives this dysfunction, which is implicated in the syndrome's pathogenesis. The hypothesis that kwashiorkor can be prevented by fortifying low quality diets with methionine, along with nutrients that support efficient methionine use, such as choline, requires further investigation.
FUNDING BACKGROUND
The Hickey Family Foundation, the American College of Gastroenterology, the NICHD, and the USDA/ARS.

Identifiants

pubmed: 35030356
pii: S2352-3964(21)00585-5
doi: 10.1016/j.ebiom.2021.103791
pmc: PMC8761690
pii:
doi:

Substances chimiques

Carbon 7440-44-0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

103791

Subventions

Organisme : NIDDK NIH HHS
ID : P30 DK056341
Pays : United States
Organisme : NICHD NIH HHS
ID : T32 HD071839
Pays : United States

Informations de copyright

Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Interests The authors have no conflicts or interests to disclose.

Auteurs

Thaddaeus May (T)

Children's Nutrition Research Center, Baylor College of Medicine, One Baylor Plaza, Houston TX, USA. Electronic address: tdmay@bcm.edu.

Bethany de la Haye (B)

Washington University in St. Louis School of Medicine, USA.

Gabrielle Nord (G)

Stanford University School of Medicine, USA.

Kevin Klatt (K)

Children's Nutrition Research Center, Baylor College of Medicine, One Baylor Plaza, Houston TX, USA; Center for Precision Environmental Health, Baylor College of Medicine.

Kevin Stephenson (K)

Washington University in St. Louis School of Medicine, USA.

Sara Adams (S)

PATH, USA.

Lucy Bollinger (L)

Washington University in St. Louis School of Medicine, USA.

Neil Hanchard (N)

National Institutes of Health, USA; National Human Genome Research Institute, Nationl Institutes of Health.

Erland Arning (E)

Center of Metabolomics, Institute of Metabolic Disease, Baylor Scott and White Research Institute.

Teodoro Bottiglieri (T)

Center of Metabolomics, Institute of Metabolic Disease, Baylor Scott and White Research Institute.

Kenneth Maleta (K)

The University of Malawi College of Medicine, Malawi.

Mark Manary (M)

Children's Nutrition Research Center, Baylor College of Medicine, One Baylor Plaza, Houston TX, USA; The University of Malawi College of Medicine, Malawi; Washington University in St. Louis School of Medicine, USA.

Farook Jahoor (F)

Children's Nutrition Research Center, Baylor College of Medicine, One Baylor Plaza, Houston TX, USA.

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Classifications MeSH