Ciprofloxacin-resistant Gram-negative isolates from a tertiary care hospital in Eastern India with novel
Ciprofloxacin
Gram-negative
Mutation
gyrA
parC
Journal
Medical journal, Armed Forces India
ISSN: 0377-1237
Titre abrégé: Med J Armed Forces India
Pays: India
ID NLM: 7602492
Informations de publication
Date de publication:
Jan 2022
Jan 2022
Historique:
received:
20
03
2019
accepted:
02
10
2019
entrez:
17
1
2022
pubmed:
18
1
2022
medline:
18
1
2022
Statut:
ppublish
Résumé
Expanded-spectrum quinolones (ciprofloxacin) are highly effective against gram-negative bacteria, but significant resistance to quinolones has been increasingly reported. We sought to evaluate the prevalence of gram-negative ciprofloxacin-resistant isolates (CRIs) from our hospital and their mechanism of action. Gram-negative CRIs were identified as per standard procedures and confirmed using the Ezy MICTM Strip (HiMedia). DNA from 67 CRIs was amplified for the quinolone resistance-determining region (QRDR) and plasmid-mediated quinolone resistance genes. Thirty isolates positive for QRDR DNA were sequenced by Sanger's method to detect mutation. Of the isolates, 42.5% were found to be CRIs, the majority (74.42%) from inpatient departments, and Ciprofloxacin resistance observed in our study was mostly due to point mutations. Hence, strategies for rational use of ciprofloxacin and adherence to the dose and duration of treatment could be helpful to prevent selection and spread of mutant CRIs/strains.
Sections du résumé
BACKGROUND
BACKGROUND
Expanded-spectrum quinolones (ciprofloxacin) are highly effective against gram-negative bacteria, but significant resistance to quinolones has been increasingly reported. We sought to evaluate the prevalence of gram-negative ciprofloxacin-resistant isolates (CRIs) from our hospital and their mechanism of action.
METHODS
METHODS
Gram-negative CRIs were identified as per standard procedures and confirmed using the Ezy MICTM Strip (HiMedia). DNA from 67 CRIs was amplified for the quinolone resistance-determining region (QRDR) and plasmid-mediated quinolone resistance genes. Thirty isolates positive for QRDR DNA were sequenced by Sanger's method to detect mutation.
RESULTS
RESULTS
Of the isolates, 42.5% were found to be CRIs, the majority (74.42%) from inpatient departments, and
CONCLUSION
CONCLUSIONS
Ciprofloxacin resistance observed in our study was mostly due to point mutations. Hence, strategies for rational use of ciprofloxacin and adherence to the dose and duration of treatment could be helpful to prevent selection and spread of mutant CRIs/strains.
Identifiants
pubmed: 35035040
doi: 10.1016/j.mjafi.2019.10.002
pii: S0377-1237(19)30105-4
pmc: PMC8737103
doi:
Types de publication
Journal Article
Langues
eng
Pagination
24-31Informations de copyright
© 2020 Director General, Armed Forces Medical Services. Published by Elsevier, a division of RELX India Pvt. Ltd.
Déclaration de conflit d'intérêts
The authors have none to declare.
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