Optical genome mapping identifies rare structural variations as predisposition factors associated with severe COVID-19.

Genetic sample Genomics Virology

Journal

iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038

Informations de publication

Date de publication:
18 Feb 2022
Historique:
received: 22 08 2021
revised: 04 11 2021
accepted: 07 01 2022
pubmed: 18 1 2022
medline: 18 1 2022
entrez: 17 1 2022
Statut: ppublish

Résumé

Impressive global efforts have identified both rare and common gene variants associated with severe COVID-19 using sequencing technologies. However, these studies lack the sensitivity to accurately detect several classes of variants, especially large structural variants (SVs), which account for a substantial proportion of genetic diversity including clinically relevant variation. We performed optical genome mapping on 52 severely ill COVID-19 patients to identify rare/unique SVs as decisive predisposition factors associated with COVID-19. We identified 7 SVs involving genes implicated in two key host-viral interaction pathways: innate immunity and inflammatory response, and viral replication and spread in nine patients, of which SVs in

Identifiants

pubmed: 35036860
doi: 10.1016/j.isci.2022.103760
pii: S2589-0042(22)00030-X
pmc: PMC8744399
doi:

Types de publication

Journal Article

Langues

eng

Pagination

103760

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM114362
Pays : United States

Informations de copyright

© 2022 The Author(s).

Déclaration de conflit d'intérêts

J.L., A.H., and A.C. are salaried employees of Bionano Genomics Inc. R.K. has received honoraria, travel funding, and research support from Illumina, Asuragen, QIAGEN, Perkin Elmer Inc, Bionano Genomics, and BMS. A.H.B. has received funding from the NIH, MDA (USA), AFM Telethon, Alexion Pharmaceuticals Inc., Audentes Therapeutics Inc., Dynacure SAS, and Pfizer Inc. He has consulted and received compensation or honoraria from Asklepios BioPharmaceutical Inc, Audentes Therapeutics, Biogen, F. Hoffman-La Roche AG, GLG Inc, Guidepoint Global, and Kate Therapeutics, and holds equity in Ballard Biologics and Kate Therapeutics.

Références

JAMA. 2020 Apr 7;323(13):1239-1242
pubmed: 32091533
J Clin Invest. 2019 Jul 29;129(9):3625-3639
pubmed: 31355779
Heliyon. 2021 Jun;7(6):e07284
pubmed: 34179538
Eur J Immunol. 2019 Mar;49(3):364-374
pubmed: 30485414
Nature. 2021 Mar;591(7848):92-98
pubmed: 33307546
Stem Cell Res Ther. 2013 Mar 21;4(2):31
pubmed: 23517567
N Engl J Med. 2020 Oct 15;383(16):1522-1534
pubmed: 32558485
J Immunol. 2017 Nov 15;199(10):3623-3633
pubmed: 29046345
Sci Rep. 2018 Aug 7;8(1):11820
pubmed: 30087386
Patterns (N Y). 2021 May 03;2(5):100248
pubmed: 34027500
Clin Infect Dis. 2021 Jul 15;73(2):328-331
pubmed: 32750119
Hum Mutat. 2007 Jul;28(7):703-9
pubmed: 17354266
Clin Kidney J. 2014 Apr;7(2):182-5
pubmed: 25852868
Nat Immunol. 2015 Mar;16(3):246-57
pubmed: 25642822
N Engl J Med. 2020 Feb 20;382(8):727-733
pubmed: 31978945
Nat Commun. 2021 Jul 28;12(1):4584
pubmed: 34321474
PeerJ. 2020 Nov 12;8:e10402
pubmed: 33240681
J Eur Acad Dermatol Venereol. 2019 Feb;33(2):e55-e57
pubmed: 30022538
JAMA. 2020 May 26;323(20):2052-2059
pubmed: 32320003
Lancet. 2020 Mar 28;395(10229):1054-1062
pubmed: 32171076
J Allergy Clin Immunol. 2020 Jul;146(1):110-118
pubmed: 32294485
Nat Commun. 2019 Mar 4;10(1):1025
pubmed: 30833565
PLoS One. 2009 May 25;4(5):e5674
pubmed: 19479062
Science. 2020 Oct 23;370(6515):
pubmed: 32972995
JAMA. 2020 Dec 1;324(21):2141-2143
pubmed: 33175133
Nucleic Acids Res. 2018 Aug 21;46(14):7236-7249
pubmed: 30137632
Am J Pathol. 2017 Apr;187(4):851-863
pubmed: 28193481
Front Cell Infect Microbiol. 2020 Jul 21;10:404
pubmed: 32793517
Gene. 2020 Sep 5;754:144821
pubmed: 32497559
Clin Exp Immunol. 2016 Jul;185(1):1-21
pubmed: 26919392
N Engl J Med. 2019 Jul 4;381(1):64-74
pubmed: 31269367
Am J Hum Genet. 2021 Aug 5;108(8):1409-1422
pubmed: 34237280
Nat Microbiol. 2020 Apr;5(4):562-569
pubmed: 32094589
N Engl J Med. 2020 Dec 17;383(25):2451-2460
pubmed: 32412710
Am J Hum Genet. 2013 Apr 4;92(4):530-46
pubmed: 23541343
Lancet Infect Dis. 2014 Feb;14(2):100-1
pubmed: 24457167
Am J Hum Genet. 2021 Aug 5;108(8):1423-1435
pubmed: 34237281
J Virol. 2015 Oct 14;90(1):92-102
pubmed: 26468529
Nature. 2020 Aug;584(7821):430-436
pubmed: 32640463
JAMA. 2020 Aug 18;324(7):663-673
pubmed: 32706371
Elife. 2021 Mar 02;10:
pubmed: 33650967
Virology. 2018 May;518:324-327
pubmed: 29587190
Cell. 2006 Feb 24;124(4):783-801
pubmed: 16497588
Genome Biol. 2019 Nov 20;20(1):246
pubmed: 31747936
Eur J Hum Genet. 2020 Jun;28(6):715-718
pubmed: 32404885
Hum Mol Genet. 2002 Oct 1;11(20):2371-5
pubmed: 12351572
Thorac Cancer. 2020 Feb;11(2):470-474
pubmed: 31908161
Synth Biol (Oxf). 2020 Jun 08;5(1):ysaa006
pubmed: 32995550
Clin Med Insights Endocrinol Diabetes. 2020 Mar 20;13:1179551420912972
pubmed: 32231442
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
G3 (Bethesda). 2015 Mar 17;5(5):931-41
pubmed: 25787242
Science. 2021 Apr 2;372(6537):
pubmed: 33632895
Int J Obes (Lond). 2020 Nov;44(11):2335-2338
pubmed: 32958905
MMWR Morb Mortal Wkly Rep. 2020 May 08;69(18):545-550
pubmed: 32379729
Nat Commun. 2019 Apr 16;10(1):1784
pubmed: 30992455
iScience. 2020 Jun 26;23(6):101160
pubmed: 32405622
Cell. 2020 May 28;181(5):1036-1045.e9
pubmed: 32416070
Orphanet J Rare Dis. 2019 Dec 3;14(1):281
pubmed: 31796081
PLoS Pathog. 2020 Oct 27;16(10):e1009035
pubmed: 33108395
Am J Hum Genet. 2021 Jul 1;108(7):1350-1355
pubmed: 34115965
Basic Res Cardiol. 2012 Jan;107(1):233
pubmed: 22167343
Am J Med Genet A. 2016 Jan;170A(1):249-53
pubmed: 26440664
J Korean Med Sci. 2020 Sep 28;35(38):e343
pubmed: 32989935
Annu Rev Immunol. 2015;33:257-90
pubmed: 25581309
J Med Virol. 2021 Apr;93(4):2476-2486
pubmed: 33404091
Science. 2020 Aug 7;369(6504):718-724
pubmed: 32661059
BMJ. 2020 May 22;369:m1985
pubmed: 32444460
Am J Hum Genet. 2020 Sep 3;107(3):381-402
pubmed: 32814065
Open Forum Infect Dis. 2020 Sep 01;7(10):ofaa401
pubmed: 33088846

Auteurs

Nikhil Shri Sahajpal (NS)

Department of Pathology, Medical College of Georgia, Augusta University, 1120 15th Street, BF-207, Augusta, GA 30912, USA.

Chi-Yu Jill Lai (CY)

Bionano Genomics, Inc., San Diego, CA 92121, USA.

Alex Hastie (A)

Bionano Genomics, Inc., San Diego, CA 92121, USA.

Ashis K Mondal (AK)

Department of Pathology, Medical College of Georgia, Augusta University, 1120 15th Street, BF-207, Augusta, GA 30912, USA.

Siavash Raeisi Dehkordi (SR)

Department of Computer Science and Engineering, University of California at San Diego, San Diego, CA 92093, USA.

Caspar I van der Made (CI)

Department of Human Genetics, Radboud University Medical Center for Infectious Diseases (RCI), Department of Internal Medicine, Radboud Institute for Molecular Life Sciences, and Radboud Expertise Center for Immunodeficiency and Autoinflammation, Radboud University Medical Center, 6525 Nijmegen, the Netherlands.

Olivier Fedrigo (O)

Vertebrate Genome Lab, The Rockefeller University, New York, NY 10065, USA.

Farooq Al-Ajli (F)

Vertebrate Genome Lab, The Rockefeller University, New York, NY 10065, USA.

Sawan Jalnapurkar (S)

Department of Medicine, Medical College of Georgia, Augusta University, GA 30912, USA.

Marta Byrska-Bishop (M)

New York Genome Center, New York, NY 10013, USA.

Rashmi Kanagal-Shamanna (R)

Hematopathology, University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.

Brynn Levy (B)

Department of Pathology and Cell Biology, Columbia University Medical Center, New York 10032, USA.

Maximilian Schieck (M)

Department of Human Genetics, Cluster of Excellence RESIST (EXC 2155), Hannover Medical School, Hannover 30625, Germany.

Thomas Illig (T)

Department of Human Genetics, Cluster of Excellence RESIST (EXC 2155), Hannover Medical School, Hannover 30625, Germany.
Hannover Unified Biobank (HUB), Hannover 30625, Germany.

Silviu-Alin Bacanu (SA)

Department of Psychiatry, Virginia Commonwealth University, Richmond, VA 23284, USA.

Janet S Chou (JS)

Division of Immunology, Boston Children's Hospital, Boston, MA 02115, USA.

Adrienne G Randolph (AG)

Department of Anesthesiology, Critical Care, and Pain Medicine, Boston Children's Hospital, Boston, MA 02115, USA.
Departments of Anesthesia and Pediatrics, Harvard Medical School, Boston, MA 02115, USA.

Amyn M Rojiani (AM)

Department of Pathology, Medical College of Georgia, Augusta University, 1120 15th Street, BF-207, Augusta, GA 30912, USA.

Michael C Zody (MC)

New York Genome Center, New York, NY 10013, USA.

Catherine A Brownstein (CA)

Division of Genetics and Genomics, The Manton Center for Orphan Disease Research, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Alan H Beggs (AH)

Division of Genetics and Genomics, The Manton Center for Orphan Disease Research, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Vineet Bafna (V)

Department of Computer Science and Engineering, University of California at San Diego, San Diego, CA 92093, USA.

Erich D Jarvis (ED)

Vertebrate Genome Lab, The Rockefeller University, New York, NY 10065, USA.
Laboratory of Neurogenetics of Language, The Rockefeller University, New York, NY 10065, USA.
Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA.

Alexander Hoischen (A)

Department of Human Genetics, Radboud University Medical Center for Infectious Diseases (RCI), Department of Internal Medicine, Radboud Institute for Molecular Life Sciences, and Radboud Expertise Center for Immunodeficiency and Autoinflammation, Radboud University Medical Center, 6525 Nijmegen, the Netherlands.

Alka Chaubey (A)

Department of Pathology, Medical College of Georgia, Augusta University, 1120 15th Street, BF-207, Augusta, GA 30912, USA.
Bionano Genomics, Inc., San Diego, CA 92121, USA.

Ravindra Kolhe (R)

Department of Pathology, Medical College of Georgia, Augusta University, 1120 15th Street, BF-207, Augusta, GA 30912, USA.

Classifications MeSH