Normal and tumor-derived organoids as a drug screening platform for tumor-specific drug vulnerabilities.
Cancer
Cell Biology
Health Sciences
High Throughput Screening
Organoids
Stem Cells
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
18 03 2022
18 03 2022
Historique:
entrez:
17
1
2022
pubmed:
18
1
2022
medline:
9
4
2022
Statut:
epublish
Résumé
Patient-derived tumor organoids can be predictive of patient's treatment responses, and normal tissue-derived organoids allow for drug toxicity testing. Combining both types of organoids therefore enables screening for tumor-specific drug vulnerabilities. Here, we provide a detailed protocol for organoid drug screening using, as proof-of-principle, patient-derived malignant rhabdoid tumor organoids. The protocol can be adapted for drug testing on any tumor and/or normal tissue-derived organoid culture. For complete details on the use and execution of this protocol, please refer to Calandrini et al. (2021).
Identifiants
pubmed: 35036959
doi: 10.1016/j.xpro.2021.101079
pii: S2666-1667(21)00785-1
pmc: PMC8752949
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101079Informations de copyright
© 2021 The Author(s).
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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