Innate and Adaptive Immunity to Transfused Allogeneic RBCs in Mice Requires MyD88.
Journal
Journal of immunology (Baltimore, Md. : 1950)
ISSN: 1550-6606
Titre abrégé: J Immunol
Pays: United States
ID NLM: 2985117R
Informations de publication
Date de publication:
15 02 2022
15 02 2022
Historique:
received:
16
08
2021
accepted:
09
12
2021
pubmed:
19
1
2022
medline:
22
2
2022
entrez:
18
1
2022
Statut:
ppublish
Résumé
RBC transfusion therapy is essential for the treatment of anemia. A serious complication of transfusion is the development of non-ABO alloantibodies to polymorphic RBC Ags; yet, mechanisms of alloantibody formation remain unclear. Storage of mouse RBCs before transfusion increases RBC immunogenicity through an unknown mechanism. We previously reported that sterile, stored mouse RBCs activate splenic dendritic cells (DCs), which are required for alloimmunization. Here we transfused mice with allogeneic RBCs to test whether stored RBCs activate pattern recognition receptors (PRRs) on recipient DCs to induce adaptive immunity. TLRs are a class of PRRs that regulate DC activation, which signal through two adapter molecules: MyD88 and TRIF. We show that the inflammatory cytokine response, DC activation and migration, and the subsequent alloantibody response to transfused RBCs require MyD88 but not TRIF, suggesting that a restricted set of PRRs are responsible for sensing RBCs and triggering alloimmunization.
Identifiants
pubmed: 35039331
pii: jimmunol.2100784
doi: 10.4049/jimmunol.2100784
pmc: PMC10107373
mid: NIHMS1763965
doi:
Substances chimiques
Biomarkers
0
Isoantibodies
0
Myd88 protein, mouse
0
Myeloid Differentiation Factor 88
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
991-997Subventions
Organisme : NHLBI NIH HHS
ID : P01 HL132819
Pays : United States
Informations de copyright
Copyright © 2022 by The American Association of Immunologists, Inc.
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