Exposure to microplastics leads to a defective ovarian function and change in cytoskeleton protein expression in rat.
Cytoskeleton
Ovary
Oxidative stress
Polystyrene-microplastics
Rat
Journal
Environmental science and pollution research international
ISSN: 1614-7499
Titre abrégé: Environ Sci Pollut Res Int
Pays: Germany
ID NLM: 9441769
Informations de publication
Date de publication:
May 2022
May 2022
Historique:
received:
02
08
2021
accepted:
15
12
2021
pubmed:
19
1
2022
medline:
11
5
2022
entrez:
18
1
2022
Statut:
ppublish
Résumé
Microplastics (MPs) are ubiquitous environmental contaminants; through their physicochemical properties, they can have potentially negative effects on the environment as well as on animal and human health. Studies addressing the toxicity of MPs on mammalian female reproduction are almost absent. Thus, the main objective of the present study was to assess the impact of oral exposure, during four estrous cycles, of 5 µm polystyrene-type microplastics (PS-MPs) on ovarian function in rats. Particles of PS-MPs were detected in the duodenum and, for the first time, in the different compartments of the ovarian tissue. The toxicity of accumulated PS-MPs was manifested by the reduced relative ovarian weights, by the alteration in the folliculogenesis and in the estrous cycle duration, and by the reduced serum concentration of estradiol. The defective ovarian function following PS-MP treatment might be due to the induction of oxidative stress, which has been proved by an increased malondialdehyde (MDA) concentration and an increased superoxide dismutase (SOD) and catalase (CAT) activities as well as a decreased protein sulfhydryl (PSH) level in the rat ovary. Importantly, by immunofluorescence and RT-PCR, we demonstrated a significant decrease in the expression of cytoskeletal proteins: α-tubulin and disheveled-associated activator of morphogenesis (DAAM-1) in the ovary of rats exposed to PS-MPs at proteomic and transcriptomic levels. Our results uncovered, for the first time, the distribution and accumulation of PS-MPs across rat ovary, revealed a significant alteration in some biomarkers of the ovarian function, and highlighted the possible involvement of MP-induced disturbance of cytoskeleton in these adverse effects.
Identifiants
pubmed: 35040070
doi: 10.1007/s11356-021-18218-3
pii: 10.1007/s11356-021-18218-3
doi:
Substances chimiques
Antioxidants
0
Microplastics
0
Plastics
0
Polystyrenes
0
Water Pollutants, Chemical
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
34594-34606Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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