PbrROP1/2-elicited imbalance of cellulose deposition is mediated by a CrRLK1L-ROPGEF module in the pollen tube of Pyrus.
Cellulose
CrRLK1L
Pear
Pollen tube growth
Rop
RopGEF
Journal
Horticulture research
ISSN: 2662-6810
Titre abrégé: Hortic Res
Pays: England
ID NLM: 101655540
Informations de publication
Date de publication:
19 Jan 2022
19 Jan 2022
Historique:
entrez:
19
1
2022
pubmed:
20
1
2022
medline:
20
1
2022
Statut:
aheadofprint
Résumé
Pollen tube growth is critical for the sexual reproduction of flowering plants. Catharanthus roseus receptor-like kinases (CrRLK1L) play an important role in plant sexual reproduction, pollen tube growth, and male and female gametophyte recognition. Here, we identified a CrRLK1L protein in pear (Pyrus bretschneideri), PbrCrRLK1L13, which is necessary for normal tip growth of pollen tube. When PbrCrRLK1L13 was knocked down, the pollen tube grew faster. Interaction analysis showed that the kinase domain of PbrCrRLK1L13 interacted with the C-terminal region of PbrGEF8, and PbrCrRLK1L13 activated the phosphorylation of PbrGEF8 in vitro. Furthermore, PbrROP1 and PbrROP2 were the downstream targets of PbrCrRLK1L13-PbrGEF8. When we knocked down the expression of PbrCrRLK1L13, PbrGEF8 or PbrROP1/2, the balance of cellulose deposition in the pollen tube wall was disrupted. Considering these factors, we proposed a model for a signaling event regulating pear pollen tube growth. During pear pollen tube elongation, PbrCrRLK1L13 acted as a surface regulator of the PbrROP1 and PbrROP2 signaling pathway via PbrGEF8 to affect the balance of cellulose deposition and regulate pear pollen tube growth.
Identifiants
pubmed: 35043175
pii: 6511232
doi: 10.1093/hr/uhab034
pmc: PMC8824538
pii:
doi:
Types de publication
Journal Article
Langues
eng
Informations de copyright
© The Author(s) 2022. Published by Oxford University Press. All rights reserved.
Références
Genomics. 2017 Jul;109(3-4):290-301
pubmed: 28502783
PLoS Biol. 2013 Nov;11(11):e1001719
pubmed: 24302886
Plant Physiol. 2001 Jun;126(2):670-84
pubmed: 11402196
PLoS Genet. 2016 Jul 22;12(7):e1006085
pubmed: 27447945
FEBS Lett. 2012 May 7;586(9):1253-8
pubmed: 22500990
Mol Plant. 2018 Sep 10;11(9):1198-1209
pubmed: 30055264
Plant Signal Behav. 2011 May;6(5):732-5
pubmed: 21455029
Photosynth Res. 2021 Jan;147(1):107-124
pubmed: 33269435
Plant Cell. 2011 Apr;23(4):1208-18
pubmed: 21478442
Proc Natl Acad Sci U S A. 2010 Oct 12;107(41):17461-2
pubmed: 20926748
Plant Physiol. 2011 Mar;155(3):1169-90
pubmed: 21205616
Mol Plant. 2013 Jul;6(4):1187-201
pubmed: 23024212
Nature. 2020 Mar;579(7800):561-566
pubmed: 32214247
Plant Cell. 2002 Sep;14(9):2277-87
pubmed: 12215520
Sci China Life Sci. 2018 Jan;61(1):100-112
pubmed: 28795376
Plant Cell. 2002 Apr;14(4):777-94
pubmed: 11971134
Science. 2017 Dec 22;358(6370):1596-1600
pubmed: 29242234
Science. 2014 Jan 24;343(6169):408-11
pubmed: 24458638
Plant Physiol. 2008 Feb;146(2):368-76
pubmed: 18065554
Trends Cell Biol. 2012 Sep;22(9):492-501
pubmed: 22795444
Development. 2003 May;130(10):2149-59
pubmed: 12668629
Science. 2007 Aug 3;317(5838):656-60
pubmed: 17673660
Plant Physiol. 2012 Dec;160(4):1940-55
pubmed: 23037507
Plant Physiol. 2016 Aug;171(4):2379-92
pubmed: 27342308
Curr Biol. 2022 Feb 7;32(3):508-517.e3
pubmed: 34875231
Genetics. 2003 Dec;165(4):2137-51
pubmed: 14704193
J Integr Plant Biol. 2013 Sep;55(9):835-46
pubmed: 23758901
Curr Biol. 2009 Aug 11;19(15):1327-31
pubmed: 19646876
J Integr Plant Biol. 2012 Mar;54(3):169-79
pubmed: 22233300
J Cell Biol. 2001 Mar 5;152(5):1019-32
pubmed: 11238457
J Cell Biol. 2005 Apr 11;169(1):127-38
pubmed: 15824136
Science. 2017 Dec 22;358(6370):1600-1603
pubmed: 29242232
Proc Natl Acad Sci U S A. 2005 Jul 26;102(30):10736-41
pubmed: 16027369
FEBS Lett. 2009 Jan 5;583(1):75-80
pubmed: 19070620
Curr Opin Plant Biol. 2012 Dec;15(6):659-69
pubmed: 22884521
Front Plant Sci. 2012 Apr 17;3:75
pubmed: 22639663
New Phytol. 2013 Dec;200(4):1089-101
pubmed: 23915272
Plant Cell. 1999 Sep;11(9):1731-42
pubmed: 10488239
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Plant Cell. 2018 May;30(5):1023-1039
pubmed: 29716992
Plant Physiol. 2003 Mar;131(3):1191-208
pubmed: 12644670
Proc Natl Acad Sci U S A. 2010 Oct 12;107(41):17821-6
pubmed: 20876100
AoB Plants. 2011;2011:plr017
pubmed: 22476487
Plant Sci. 2015 Aug;237:93-107
pubmed: 26089155
J Exp Bot. 2003 Jan;54(380):93-101
pubmed: 12456759
Development. 2009 Oct;136(19):3279-88
pubmed: 19736323
J Exp Bot. 2013 Dec;64(18):5599-610
pubmed: 24136420