[Co-Culturing of Osteoblasts and Chondrocytes Upregulates HIF-1 Pathway of Chondrocytes via MAPK Signaling].
Chondrocyte
Co-culture
HIF-1α
ROS
Journal
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
ISSN: 1672-173X
Titre abrégé: Sichuan Da Xue Xue Bao Yi Xue Ban
Pays: China
ID NLM: 101162609
Informations de publication
Date de publication:
Jan 2022
Jan 2022
Historique:
entrez:
20
1
2022
pubmed:
21
1
2022
medline:
22
1
2022
Statut:
ppublish
Résumé
To study the effect of co-culturing chondrocytes with osteoblasts on hypoxia-inducible factor (HIF)-1 pathway of chondrocytes and its mechanism. Chondrocytes and osteoblasts were separately extracted from the knee joint and skull of newborn mice by trypsin digestion. The co-culturing system of osteoblasts and chondrocytes was constructed by using Transwell inserts to culture the osteoblasts and 6-well plate to culture the chondrocytes. We used qRT-PCR to examine changes in the mRNA expression of HIFs and its target gene pyruvate dehydrogenase kinase 1 ( The results of qRT-PCR and Western blot showed upregulated levels of HIF-1α gene and protein expression ( Co-culturing with osteoblasts enhanced the ERK1/2 and p38 signaling of chondrocytes and upregulated the HIF-1 pathway of chondrocytes.
Identifiants
pubmed: 35048606
doi: 10.12182/20220160104
pmc: PMC10408868
doi:
Substances chimiques
Hypoxia-Inducible Factor 1, alpha Subunit
0
Mitogen-Activated Protein Kinases
EC 2.7.11.24
Types de publication
Journal Article
Langues
chi
Sous-ensembles de citation
IM
Pagination
92-97Informations de copyright
Copyright© by Editorial Board of Journal of Sichuan University (Medical Sciences).
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