Monitoring tissue engineered constructs and protocols with laboratory-based x-ray phase contrast tomography.

Humans Image Processing, Computer-Assisted Microscopy, Phase-Contrast Tissue Engineering / methods Tomography, X-Ray Computed / methods X-Rays
3D-imaging In-vitro organ maturation Laboratory-based phase contrast computed tomography (PC-CT) Regenerative medicine

Journal

Acta biomaterialia
ISSN: 1878-7568
Titre abrégé: Acta Biomater
Pays: England
ID NLM: 101233144

Informations de publication

Date de publication:
15 03 2022
Historique:
received: 13 10 2021
revised: 21 12 2021
accepted: 12 01 2022
pubmed: 21 1 2022
medline: 12 4 2022
entrez: 20 1 2022
Statut: ppublish

Résumé

Tissue engineering (TE) aims to generate bioengineered constructs which can offer a surgical treatment for many conditions involving tissue or organ loss. Construct generation must be guided by suitable assessment tools. However, most current tools (e.g. histology) are destructive, which restricts evaluation to a single-2D anatomical plane, and has no potential for assessing constructs prior to or following their implantation. An alternative can be provided by laboratory-based x-ray phase contrast computed tomography (PC-CT), which enables the extraction of 3D density maps of an organ's anatomy. In this work, we developed a semi-automated image processing pipeline dedicated to the analysis of PC-CT slices of oesophageal constructs. Visual and quantitative (density and morphological) information is extracted on a volumetric basis, enabling a comprehensive evaluation of the regenerated constructs. We believe the presented tools can enable the successful regeneration of patient-specific oesophagus, and bring comparable benefit to a wide range of TE applications. STATEMENT OF SIGNIFICANCE: Phase contrast computed tomography (PC-CT) is an imaging modality which generates high resolution volumetric density maps of biological tissue. In this work, we demonstrate the use of PC-CT as a new tool for guiding the progression of an oesophageal tissue engineering (TE) protocol. Specifically, we developed a semi-automated image-processing pipeline which analyses the oesophageal PC-CT slices, extracting visual and quantitative (density and morphological) information. This information was proven key for performing a comprehensive evaluation of the regenerated constructs, and cannot be obtained through existing assessment tools primarily due to their destructive nature (e.g. histology). This work paves the way for using PC-CT in a wide range of TE applications which can be pivotal for unlocking the potential of this field.

Identifiants

DOI: 10.1016/j.actbio.2022.01.022 PMID: 35051630
pubmed: 35051630
pii: S1742-7061(22)00031-9
doi: 10.1016/j.actbio.2022.01.022
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

290-299

Subventions

Organisme : Department of Health
ID : NIHR-RP-2014-04-046
Pays : United Kingdom

Informations de copyright

Copyright © 2022. Published by Elsevier Ltd.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare no competing interests.

Auteurs

Savvas Savvidis (S)

Department of Medical Physics and Biomedical Engineering, University College London, London, WC1E 6BT, UK. Electronic address: savvas.savvides.12@ucl.ac.uk.

Mattia F M Gerli (MFM)

Stem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College London, London, WC1N 1EH, UK; UCL Division of Surgery and Interventional Science, Royal Free Hospital, London, UK. Electronic address: m.gerli@ucl.ac.uk.

Marco Pellegrini (M)

Stem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College London, London, WC1N 1EH, UK. Electronic address: marco.pellegrini@ucl.ac.uk.

Lorenzo Massimi (L)

Department of Medical Physics and Biomedical Engineering, University College London, London, WC1E 6BT, UK. Electronic address: l.massimi@ucl.ac.uk.

Charlotte K Hagen (CK)

Department of Medical Physics and Biomedical Engineering, University College London, London, WC1E 6BT, UK. Electronic address: charlotte.hagen.10@ucl.ac.uk.

Marco Endrizzi (M)

Department of Medical Physics and Biomedical Engineering, University College London, London, WC1E 6BT, UK. Electronic address: m.endrizzi@ucl.ac.uk.

Alessia Atzeni (A)

Centre for Medical Image Computing, Department of Medical Physics and Biomedical Engineering, University College London, WC1E 6BT, UK. Electronic address: alessia.atzeni.14@ucl.ac.uk.

Olumide K Ogunbiyi (OK)

Department of Histopathology, Level 3, Camelia Botnar Laboratories, Great Ormond Street Hospital, London, WC1N 3NN, UK. Electronic address: olumide.ogunbiyi@gosh.nhs.uk.

Mark Turmaine (M)

Division of Biosciences, University College London, London, WC1E 6DE, UK. Electronic address: m.turmaine@ucl.ac.uk.

Elizabeth S Smith (ES)

Division of Biosciences, University College London, London, WC1E 6DE, UK. Electronic address: e.slavik-smith@ucl.ac.uk.

Claudio Fagiani (C)

Stem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College London, London, WC1N 1EH, UK. Electronic address: claudio.fagiani@mail.polimi.it.

Giulia Selmin (G)

Stem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College London, London, WC1N 1EH, UK. Electronic address: g.selmin@ucl.ac.uk.

Luca Urbani (L)

Stem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College London, London, WC1N 1EH, UK. Electronic address: luca.urbani@researchinliver.org.uk.

Natalie Durkin (N)

Stem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College London, London, WC1N 1EH, UK. Electronic address: n.durkin@ucl.ac.uk.

Soichi Shibuya (S)

Stem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College London, London, WC1N 1EH, UK. Electronic address: s.shibuya@ucl.ac.uk.

Paolo De Coppi (P)

Stem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College London, London, WC1N 1EH, UK; Specialist Neonatal and Paediatric Surgery, Great Ormond Street Hospital NHS Trust, UK. Electronic address: paolo.decoppi@gosh.nhs.uk.

Alessandro Olivo (A)

Department of Medical Physics and Biomedical Engineering, University College London, London, WC1E 6BT, UK. Electronic address: a.olivo@ucl.ac.uk.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Monitoring tissue engineered constructs and...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Traitement d'image par ordinateur › Monitoring tissue engineered constructs and...
questionsmedicales.fr › Techniques d'investigation › Microscopie interférentielle › Microscopie de contraste de phase › Monitoring tissue engineered constructs and...
questionsmedicales.fr › Technologie, industrie et agriculture › Ingénierie › Bioingénierie › Ingénierie cellulaire › Ingénierie tissulaire › Monitoring tissue engineered constructs and...
questionsmedicales.fr › Diagnostic › Techniques et procédures diagnostiques › Imagerie diagnostique › Tomographie › Tomographie à rayons X › Tomodensitométrie › Monitoring tissue engineered constructs and...
questionsmedicales.fr › Phénomènes physiques › Rayonnement › Rayonnement ionisant › Rayons X › Monitoring tissue engineered constructs and...