Aerobic Vaginitis Diagnosis Criteria Combining Gram Stain with Clinical Features: An Establishment and Prospective Validation Study.
16S rRNA gene sequencing
Gram stain
aerobic vaginitis
clinical features
diagnostic criteria
Journal
Diagnostics (Basel, Switzerland)
ISSN: 2075-4418
Titre abrégé: Diagnostics (Basel)
Pays: Switzerland
ID NLM: 101658402
Informations de publication
Date de publication:
13 Jan 2022
13 Jan 2022
Historique:
received:
13
11
2021
revised:
06
01
2022
accepted:
10
01
2022
entrez:
21
1
2022
pubmed:
22
1
2022
medline:
22
1
2022
Statut:
epublish
Résumé
Wet-mount microscopy aerobic vaginitis (AV) diagnostic criteria need phase-contrast microscopy and keen microscopists, and the preservation of saline smears is less common in clinical practice. This research work developed new AV diagnostic criteria that combine Gram stain with clinical features. We enrolled 325 AV patients and 325 controls as a study population to develop new AV diagnostic criteria. Then, an independent group, which included 500 women, was used as a validation population. AV-related microscopic findings on Gram-stained and wet-mount smears from the same participants were compared. The accuracy of bacterial indicators from the two methods was verified by bacterial 16S rRNA V4 sequencing (
Identifiants
pubmed: 35054351
pii: diagnostics12010185
doi: 10.3390/diagnostics12010185
pmc: PMC8775230
pii:
doi:
Types de publication
Journal Article
Langues
eng
Références
J Infect Dev Ctries. 2020 Jan 31;14(1):48-58
pubmed: 32088684
Arch Gynecol Obstet. 2016 Jul;294(1):109-14
pubmed: 26781259
Antibiotics (Basel). 2021 Sep 20;10(9):
pubmed: 34572715
J Clin Microbiol. 2007 Apr;45(4):1113-7
pubmed: 17301283
Antibiotics (Basel). 2021 Dec 19;10(12):
pubmed: 34943764
Eur J Clin Microbiol Infect Dis. 2016 Aug;35(8):1297-303
pubmed: 27180245
Arch Gynecol Obstet. 2015 Feb;291(2):251-7
pubmed: 25367602
Eur J Clin Microbiol Infect Dis. 2019 Feb;38(2):233-239
pubmed: 30467614
Int J STD AIDS. 2020 Oct;31(11):1018-1027
pubmed: 32842907
BJOG. 2002 Jan;109(1):34-43
pubmed: 11845812
Diagnostics (Basel). 2020 Oct 28;10(11):
pubmed: 33126716
Int J Gynaecol Obstet. 2001 Jul;74(1):35-43
pubmed: 11430939
Ginekol Pol. 2013 May;84(5):352-8
pubmed: 23819400
Diagn Microbiol Infect Dis. 2016 Jun;85(2):227-30
pubmed: 27112831
Obstet Gynecol. 2001 Mar;97(3):482
pubmed: 11256384
Clin Microbiol Infect. 2020 Oct;26(10):1318-1323
pubmed: 32213317
Int J STD AIDS. 2018 Nov;29(13):1258-1272
pubmed: 30049258
Infect Dis Obstet Gynecol. 1996;4(1):2-6
pubmed: 18476056
J Clin Microbiol. 2019 Aug 26;57(9):
pubmed: 31315951
BJOG. 2009 Sep;116(10):1315-24
pubmed: 19538417
J Clin Pathol. 2000 Apr;53(4):308-13
pubmed: 10823128
MMWR Recomm Rep. 2015 Jun 5;64(RR-03):1-137
pubmed: 26042815
Front Cell Infect Microbiol. 2021 Sep 09;11:677648
pubmed: 34568084
Int J Antimicrob Agents. 2004 Jul;24(1):85-8
pubmed: 15225868
Arch Gynecol Obstet. 2012 Apr;285(4):1013-8
pubmed: 21938501
Diagn Microbiol Infect Dis. 2020 Apr;96(4):114981
pubmed: 31955954
Arch Gynecol Obstet. 2013 Feb;287(2):329-35
pubmed: 23015152
Obstet Gynecol. 1990 Oct;76(4):727-30
pubmed: 2216211
Taiwan J Obstet Gynecol. 2016 Feb;55(1):40-4
pubmed: 26927246
Microorganisms. 2020 Jun 29;8(7):
pubmed: 32610432
Sex Transm Infect. 2017 Feb;93(1):8-10
pubmed: 27272705
J Clin Microbiol. 2021 Jan 21;59(2):
pubmed: 33148709
Res Microbiol. 2017 Nov - Dec;168(9-10):845-858
pubmed: 28502874
Eur J Clin Microbiol Infect Dis. 2016 Apr;35(4):657-64
pubmed: 26810061
Crit Rev Microbiol. 2016 May;42(3):352-63
pubmed: 25834920