Endogenous Nitric Oxide-Releasing Microgel Coating Prevents Clot Formation on Oxygenator Fibers Exposed to In Vitro Blood Flow.
ARDS
PMP fibers
anti-thrombogenicity
blood-contacting surfaces
microgel coating
platelet activation
systemic anticoagulation
Journal
Membranes
ISSN: 2077-0375
Titre abrégé: Membranes (Basel)
Pays: Switzerland
ID NLM: 101577807
Informations de publication
Date de publication:
06 Jan 2022
06 Jan 2022
Historique:
received:
26
11
2021
revised:
23
12
2021
accepted:
28
12
2021
entrez:
21
1
2022
pubmed:
22
1
2022
medline:
22
1
2022
Statut:
epublish
Résumé
Clot formation on foreign surfaces of extracorporeal membrane oxygenation systems is a frequent event. Herein, we show an approach that mimics the enzymatic process of endogenous nitric oxide (NO) release on the oxygenator membrane via a biomimetic, non-fouling microgel coating to spatiotemporally inhibit the platelet (PLT) activation and improve antithrombotic properties. This study aims to evaluate the potential of this biomimetic coating towards NO-mediated PLT inhibition and thereby the reduction of clot formation under flow conditions. Microgel-coated (NOrel) or bare (Control) poly(4-methyl pentene) (PMP) fibers were inserted into a test channel and exposed to a short-term continuous flow of human blood. The analysis included high-resolution PLT count, pooled PLT activation via β-Thromboglobulin (β-TG) and the visualization of remnants and clots on the fibers using scanning electron microscopy (SEM). In the Control group, PLT count was significantly decreased, and β-TG concentration was significantly elevated in comparison to the NOrel group. Macroscopic and microscopic visualization showed dense layers of stable clots on the bare PMP fibers, in contrast to minimal deposition of fibrin networks on the coated fibers. Endogenously NO-releasing microgel coating inhibits the PLT activation and reduces the clot formation on PMP fibers under dynamic flow.
Sections du résumé
BACKGROUND
BACKGROUND
Clot formation on foreign surfaces of extracorporeal membrane oxygenation systems is a frequent event. Herein, we show an approach that mimics the enzymatic process of endogenous nitric oxide (NO) release on the oxygenator membrane via a biomimetic, non-fouling microgel coating to spatiotemporally inhibit the platelet (PLT) activation and improve antithrombotic properties. This study aims to evaluate the potential of this biomimetic coating towards NO-mediated PLT inhibition and thereby the reduction of clot formation under flow conditions.
METHODS
METHODS
Microgel-coated (NOrel) or bare (Control) poly(4-methyl pentene) (PMP) fibers were inserted into a test channel and exposed to a short-term continuous flow of human blood. The analysis included high-resolution PLT count, pooled PLT activation via β-Thromboglobulin (β-TG) and the visualization of remnants and clots on the fibers using scanning electron microscopy (SEM).
RESULTS
RESULTS
In the Control group, PLT count was significantly decreased, and β-TG concentration was significantly elevated in comparison to the NOrel group. Macroscopic and microscopic visualization showed dense layers of stable clots on the bare PMP fibers, in contrast to minimal deposition of fibrin networks on the coated fibers.
CONCLUSION
CONCLUSIONS
Endogenously NO-releasing microgel coating inhibits the PLT activation and reduces the clot formation on PMP fibers under dynamic flow.
Identifiants
pubmed: 35054599
pii: membranes12010073
doi: 10.3390/membranes12010073
pmc: PMC8779597
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : RO 2000/23-1
Organisme : Deutsche Forschungsgemeinschaft
ID : SI 2164/2-1
Références
Macromol Biosci. 2019 Sep;19(9):e1900125
pubmed: 31361077
Bioconjug Chem. 2019 Nov 20;30(11):2859-2869
pubmed: 31577418
Arterioscler Thromb Vasc Biol. 2001 Jul;21(7):1094-6
pubmed: 11451734
Lancet Respir Med. 2019 Feb;7(2):163-172
pubmed: 30642776
J Colloid Interface Sci. 2021 Nov;601:604-616
pubmed: 34116469
Biomaterials. 2018 Jun;166:79-95
pubmed: 29549767
Circulation. 2010 Nov 16;122(20):2068-77
pubmed: 21098468
JACC Heart Fail. 2018 Jun;6(6):503-516
pubmed: 29655828
Mol Aspects Med. 2009 Feb-Apr;30(1-2):1-12
pubmed: 18796312
Perfusion. 2021 Jan 21;:267659121989231
pubmed: 33475044
Langmuir. 2019 Feb 5;35(5):1181-1191
pubmed: 30265555
Biochim Biophys Acta. 1999 May 5;1411(2-3):273-89
pubmed: 10320663
Ann Thorac Surg. 1996 Apr;61(4):1194-8
pubmed: 8607682
Free Radic Biol Med. 2004 Oct 1;37(7):926-36
pubmed: 15336308
Artif Organs. 2020 Jul;44(7):717-726
pubmed: 31970795
Annu Int Conf IEEE Eng Med Biol Soc. 2015;2015:262-5
pubmed: 26736250
Thorac Cardiovasc Surg. 2005 Dec;53(6):389-90
pubmed: 16311981
Biomaterials. 2007 Jan;28(1):19-27
pubmed: 16959311
Crit Care Resusc. 2013 Sep;15(3):172-8
pubmed: 23944202
J Mater Chem B. 2014 Jan 28;2(4):341-356
pubmed: 32261379
N Engl J Med. 2018 May 24;378(21):1965-1975
pubmed: 29791822
J Mater Chem B. 2014 Feb 28;2(8):1059-1067
pubmed: 24634777
Front Bioeng Biotechnol. 2018 Jul 16;6:99
pubmed: 30062094
Biomaterials. 2015 Sep;63:80-92
pubmed: 26093790
Semin Thromb Hemost. 2005;31(4):381-92
pubmed: 16149014
J Biol Chem. 1991 Jun 15;266(17):11227-33
pubmed: 2040630
J Control Release. 2015 Jul 28;210:179-88
pubmed: 26004323
Eur J Clin Invest. 1999 Apr;29(4):344-50
pubmed: 10231347
Artif Organs. 2016 Mar;40(3):288-93
pubmed: 26411987
J Thromb Haemost. 2015 Jun;13 Suppl 1:S72-81
pubmed: 26149053
Small. 2020 Jul;16(27):e1907635
pubmed: 32372556
ACS Biomater Sci Eng. 2017 Mar 13;3(3):349-359
pubmed: 28317023
Biomaterials. 1982 Jan;3(1):9-15
pubmed: 7039708
Angew Chem Int Ed Engl. 2019 Jul 15;58(29):9791-9796
pubmed: 30997726
Naunyn Schmiedebergs Arch Pharmacol. 2020 Feb;393(2):287-302
pubmed: 31853617
Angew Chem Int Ed Engl. 2017 Jun 19;56(26):7380-7386
pubmed: 28524364
FEBS Lett. 1996 Mar 18;382(3):223-8
pubmed: 8605974
Science. 1992 Dec 18;258(5090):1898-902
pubmed: 1281928
Circ Res. 2001 Apr 27;88(8):756-62
pubmed: 11325866
Biomacromolecules. 2017 Sep 11;18(9):2789-2798
pubmed: 28745493
Biomater Sci. 2016 Aug 19;4(8):1161-83
pubmed: 27226170
Heart. 2003 May;89(5):535-7
pubmed: 12695459
J Thorac Dis. 2015 Jul;7(7):E166-76
pubmed: 26380745
Soft Matter. 2019 Apr 10;15(15):3227-3240
pubmed: 30916678
Biomaterials. 2005 May;26(14):1685-93
pubmed: 15576142
Front Med (Lausanne). 2018 Nov 20;5:321
pubmed: 30525038
Biomaterials. 2004 Nov;25(26):5681-703
pubmed: 15147815